Home | Community | Message Board


Magic-Mushrooms-Shop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Microscope

Jump to first unread post. Pages: 1
OfflineHydroSplash
Sporulating
Male


Registered: 07/20/09
Posts: 159
Last seen: 5 years, 8 months
Room sterilizing right now, QUESTIONS!!
    #11254198 - 10/15/09 05:44 PM (8 years, 1 month ago)

I knocked up my first jars a couple weeks back with B+, and they are about 25% colonization, so I am keeping my fingers crossed.  It was a very dirty method of inoculation because lids had to be removed because inoculation points were too small.  So I made my inoculation ports bigger this time.
  I REALLY, REALLY don't want to mess up my Golden Teacher syringe, so I'm going to ask a question that many of you will say "it's been asked before." Since it was my first inoculation, I had a shaky hand and 5 jars ended up using 10cc total.  Should I really be so concerned to only get a 1/4 cc per hole ((4 holes)1cc per jar)? In the jelly jars I bought, 1/4 cc is barely enough to see running down the side.  I really want to try my hand at Liquid Culture...Use half on the jars, or use the whole syringe on the jars...what do you guys say?


--------------------
Currently building a library of edible/active strains, please see my Trade List
If you can help in this process, the please feel free to send me a :pm:
H.S.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
Trusted Cultivator
Re: Room sterilizing right now, QUESTIONS!! [Re: HydroSplash]
    #11254218 - 10/15/09 05:47 PM (8 years, 1 month ago)

Well 1/4 cc per hole is a standard any more and its a waste of spores. 1 syringe can last me 10-12 jars so yea less is more


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Room sterilizing right now, QUESTIONS!! [Re: HydroSplash]
    #11254226 - 10/15/09 05:48 PM (8 years, 1 month ago)

5 jars 10cc's to me that works out to .5cc a hole with four holes no matter really you are fine.  Learn to make an LC its really easy and worth the four dollars you will spend to make it


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNuzzle
Misunderstood
 User Gallery


Registered: 09/29/08
Posts: 840
Last seen: 8 years, 1 day
Re: Room sterilizing right now, QUESTIONS!! [Re: truskool]
    #11254243 - 10/15/09 05:51 PM (8 years, 1 month ago)

I would say if you really wanna try the LC... Search for the "microwave LC tek"
That was the first tek I used to make LC, and I never once had a problem following that to the T... Once you have made LC you don't have to worry about running outta your syringe. :smile:

However, just relax and calm that shaky hand. You dont need much spores to get the process started.


--------------------
:hi:**If you have any spare spore prints lying around, and your feeling 'giving' drop me a PM** :dancer:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrandNewbie
Captain
Male


Registered: 05/21/08
Posts: 2,920
Loc: U.S.A.
Last seen: 3 years, 2 months
Re: Room sterilizing right now, QUESTIONS!! [Re: HydroSplash]
    #11254273 - 10/15/09 05:55 PM (8 years, 1 month ago)

The more spores in the jar, the more "struggling" has to take place to become the dominant player in your jar. Spores meet and mate. It's really a complex process. It's not like planting an acorn and getting a tree.

At some point, the amount of spores that you over inject are just wasted.

As you said, you "REALLY, REALLY don't want to mess up" your GT syringe.

There are millions of spores in that syringe. Think about it. Spores are microscopic. That means that you can NOT see them with the naked eye. So ask yourself this, "How many microscopic things does there have to be in order for me to see them with no aid, just like I can see the spore cloud in this syringe?"

I only inject in two holes now. I tried one hole per jar, but it was too slow for me. Two injections per jar opposite each other works well. 1/2cc per injection site. Plenty.

:goodluck:


--------------------
Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGanja420Boy
Heroic Doser
Male User Gallery


Registered: 11/15/06
Posts: 900
Loc: USA,Tennessee Flag
Last seen: 2 months, 27 days
Re: Room sterilizing right now, QUESTIONS!! [Re: Nuzzle]
    #11254291 - 10/15/09 05:57 PM (8 years, 1 month ago)

I think growing in LC sucks. You would be better off using the grocery store agar tek with 1 drop of spore solution and wait for the mycelium to grow, then inject sterile water into it.

With this way you have way less chance of contamination :thumbup: And 1 drop of spores easily makes 4-5 syringes.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
Trusted Cultivator
Re: Room sterilizing right now, QUESTIONS!! [Re: Ganja420Boy]
    #11254338 - 10/15/09 06:04 PM (8 years, 1 month ago)

LC are good for turning alittle into a lot fairly easy without even needing a glove box or flowhood


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Room sterilizing right now, QUESTIONS!! [Re: Ganja420Boy]
    #11254392 - 10/15/09 06:13 PM (8 years, 1 month ago)

Quote:

Ganja420Boy said:
I think growing in LC sucks. You would be better off using the grocery store agar tek with 1 drop of spore solution and wait for the mycelium to grow, then inject sterile water into it.

With this way you have way less chance of contamination :thumbup: And 1 drop of spores easily makes 4-5 syringes.




I definitely agree with this.

I am basically a noob, but delving into cloning, liquid cultures, and agar.

I have not had much luck with karo lc at all. But my very first batch of agar turned out excellent. The cloning process was kinda easy and fun. And the agar transfers turned out much more successfully than the lc attempt.

I think the reason is that as a noob one might screw it up. If they screw up an lc, its a ruined batch, whereas with transfers you get more chances to get it right. I know you're sposed to test the lc on 1 jar first , before you inoc a batch, but some people, like me, are impatient. They throw a hail mary and noc up a whole batch and hope for the best. That's what i did anyway...

I did like doing the grain liquid culture tho.

Here is good reading for learning agar:
Fastfred's Media Cookbook

Grocery Store Agar Tek
Noobie attempts strain isolation.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Edited by anonjon (10/15/09 06:20 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Room sterilizing right now, QUESTIONS!! [Re: anonjon]
    #11254416 - 10/15/09 06:16 PM (8 years, 1 month ago)

I really hate to say this but if you had listened to the advice of countless ppl on this forum you might not have had such a bad experience with LC's.  IMO they rock agar has other uses it does work for this for sure but agar is way easier to contaim


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleHerbal_Elixer
Strangerest
 User Gallery


Registered: 05/05/09
Posts: 1,841
Loc: Reykjavik
Re: Room sterilizing right now, QUESTIONS!! [Re: truskool]
    #11254603 - 10/15/09 06:44 PM (8 years, 1 month ago)

Quote:

truskool said:
I really hate to say this but if you had listened to the advice of countless ppl on this forum you might not have had such a bad experience with LC's.  IMO they rock agar has other uses it does work for this for sure but agar is way easier to contaim





That


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Microscope

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Glovebox jar sterilization question LoganSmith 542 10 09/16/09 04:45 PM
by EdgeChaos
* Sterilization question flyskimmah 907 8 04/11/04 04:14 AM
by Magash
* Sterilization question Gahr 1,315 14 01/01/09 04:50 PM
by Shr00mZ
* Syringes, Sterility question bent 648 4 07/28/04 06:51 PM
by meanoldman
* Sterilization Question!!!!!!!!!! PooGrowerPart2 315 1 03/09/05 01:15 PM
by NewfoundFreedom
* Needle Sterilization Question RageOholic67 1,977 14 11/08/02 06:19 PM
by RageOholic67
* Sterilization Question Slick37c 535 8 03/27/07 11:31 AM
by deucedbi9
* How long is too long? (a sterilization question) BladeLSD 730 7 05/29/03 10:15 PM
by Prisoner#1

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
478 topic views. 19 members, 69 guests and 24 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.005 seconds on 19 queries.