Home | Community | Message Board


High Mountain Compost
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars

Jump to first unread post. Pages: 1
OfflineAllourep
Allourep
 User Gallery

Registered: 09/23/09
Posts: 169
Loc: USA
Last seen: 1 year, 3 months
Needle sterlization
    #11254096 - 10/15/09 05:30 PM (8 years, 2 months ago)

I have heard about using a flame to sterilize your inoculating needle.  I have been advised to heat it until the syringe is red hot and then to let it cool down becuase the heat kills the spores.  So before inoculating you would heat the needle.  Then I was advised to repeat the process throughout the inoculation. It is here were I am confused. When inoculating with a syringe, you only squirt  a very miniscule amount of fluid into the spot in your jar.  Usually a 1/4 cc,4 times per jar to make 1 cc, barely enough to displace all the fluid that was previously stored in the tiny volume of the needle. Since heat kills the delicate spores, wouldn't flame sterlization in between jars kill all the spores in the needle that you're just about to inject?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Needle sterlization [Re: Allourep]
    #11254141 - 10/15/09 05:34 PM (8 years, 2 months ago)

nope..
as soon as any water goes thru the needle, it cools and your spores are fine.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
Trusted Cultivator
Re: Needle sterlization [Re: Allourep]
    #11254159 - 10/15/09 05:36 PM (8 years, 2 months ago)

Yea
Quote:

1 cc, barely enough to displace all the fluid that was previously stored in the tiny volume of the needle




1 cc is wayyyyy more then what the needle can hole its all good spores are are fine


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAllourep
Allourep
 User Gallery

Registered: 09/23/09
Posts: 169
Loc: USA
Last seen: 1 year, 3 months
Re: Needle sterlization [Re: Shea25]
    #11255291 - 10/15/09 08:31 PM (8 years, 2 months ago)

Oh,  I was trying to say 1/4 cc.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Needle sterlization [Re: Allourep]
    #11255299 - 10/15/09 08:32 PM (8 years, 2 months ago)

no matter.. its fine, we all do it..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemilkman59
milkman
Male User Gallery
Registered: 02/01/09
Posts: 267
Last seen: 8 years, 1 month
Re: Needle sterlization [Re: Slimz]
    #11255321 - 10/15/09 08:36 PM (8 years, 2 months ago)

i dont flame sterilize if the needle was sterilized right from the start. meaning pc'd for 20 min @ 15 psi.

i just used 70% alcohol. even when i clone.


--------------------
milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
Trusted Cultivator
Re: Needle sterlization [Re: milkman59]
    #11255333 - 10/15/09 08:37 PM (8 years, 2 months ago)

I disagree always Flame sterilize


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Needle sterlization [Re: Shea25]
    #11255359 - 10/15/09 08:42 PM (8 years, 2 months ago)

i disagree..

don't not always flame sterilize sometimes, but only slightly, but never after not forgetting to never not flame sterilize afterwards.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePfffffff
I am sofa king we todd ed

Registered: 10/02/09
Posts: 486
Loc: Somewhere that is fiction...
Last seen: 4 years, 6 months
Re: Needle sterlization [Re: Slimz]
    #11255369 - 10/15/09 08:46 PM (8 years, 2 months ago)

I think my head just exploded.

:eek:


--------------------
****************************************************
I am part of this community as a role playing
character.  All information is gathered on the
internet.  In no way, shape, or form is any of what
I say truthful or real life experience.  Any advice
given or received is purely for entertainment value
and not intended for any kind of illegal activity.
****************************************************


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 2 months, 12 days
Re: Needle sterlization [Re: Shea25]
    #11255392 - 10/15/09 08:49 PM (8 years, 2 months ago)

I don't flame sterilise as it melts my needles
They re made for people so i use one pre sterilized 23 1/2 sharp for each 20cc spore syringe when inoc'ing.

I also use a fresh drawing up 18 gauge when making syringes.

I only ever reuse my 18 Gauges for drawing up boiling water for later use in MS syringes, don't really wanna make a shitty spore syringe <3.

In summary, i get by with almost no contamination on just using alcohol wipe down between jars, just do everything in a glovebox with alcohol and no flame, and you should be right. I may just be lucky though.


--------------------
First we must learn...

Then... WE CAN TEACH :weirdeyes:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSA5M
Rapid Colonizer
 User Gallery


Registered: 08/12/09
Posts: 60
Loc: Makyo Star
Last seen: 8 years, 1 month
Re: Needle sterlization [Re: Shea25]
    #11255613 - 10/15/09 09:29 PM (8 years, 2 months ago)

Quote:

Shea25 said:
I disagree always Flame sterilize



ALWAYS :thumbup:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleambargh
 User Gallery


Registered: 08/15/09
Posts: 3,433
Re: Needle sterlization [Re: Allourep]
    #11255651 - 10/15/09 09:34 PM (8 years, 2 months ago)

Quote:

Allourep said:
... Since heat kills the delicate spores, wouldn't flame sterlization in between jars kill all the spores in the needle that you're just about to inject?




It evaporates them right out of the needle. After you let it cool down, the spores come out of the nice, liquid filled syringe through the empty needle.


--------------------
"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

ambargh's easy agar


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemilkman59
milkman
Male User Gallery
Registered: 02/01/09
Posts: 267
Last seen: 8 years, 1 month
Re: Needle sterlization [Re: Shea25]
    #11258968 - 10/16/09 12:27 PM (8 years, 2 months ago)

Quote:

Shea25 said:
I disagree always Flame sterilize




to each their own. i never flame sterilize everyone has their methods . ive only had 1 contamed jar in the last 2 years. 70% alcohol is good enough for me along with my pc.


--------------------
milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Flame Needle With Candle vs. Alcohol Lamp mewkiebear 2,358 8 04/23/09 02:04 AM
by Tunesy
* Cig lighter for needle sterilization, or only alcohol lamp? lsg1 2,072 2 04/14/07 10:35 AM
by Xeluc
* H202+Rubbing Alcohol mist solution=??? Gloopy 1,737 2 03/01/08 12:37 PM
by RogerRabbit
* needle sterilization question prajnaparamita 1,434 6 08/01/06 05:45 PM
by prajnaparamita
* Needle Flame and Alcohol iMtRiPpIn 1,169 12 09/20/08 03:13 AM
by RogerRabbit
* Use of Alcohol? JoelLoveland5 741 8 01/18/07 04:54 PM
by nugjug
* Flame needle procedure highc 632 2 09/26/07 03:51 PM
by veda_sticks
* No Alcohol??? bftiedt 1,104 14 12/03/09 10:15 AM
by Sillicybin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
515 topic views. 13 members, 68 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
World Seed Supply
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.004 seconds on 19 queries.