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Offlineblujay
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Need to expand my myc bank
    #11252573 - 10/15/09 01:52 PM (8 years, 2 months ago)

I tried spore prints, they were contamed 4/5 times. Also, they took forever to get rolling. So i want to just clone what I already have.

I was planning on taking my largest fruit and doing a biopsy parallel the length of the stem's core, then injecting that into a jar of sterile water.

How long should I wait before I use it to knock up grain jars... Do I need honey in it>? How do you measure honey out accurately without a scale?

Could I just hack a chunk off one of my cakes, put it in a jar of sterilized water, and break that up and then innoc the grains with that?


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InvisibleShea25
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Re: Need to expand my myc bank [Re: blujay]
    #11252579 - 10/15/09 01:53 PM (8 years, 2 months ago)

4 grams of honey or karo to 100 ml of water. I wait 14 days after innoc to use it.

Always add something shouldn't just have water as an LC

LC= Liquid Culture thats what you want to research and make


Edited by Shea25 (10/15/09 01:54 PM)


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InvisibleShea25
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Re: Need to expand my myc bank [Re: blujay]
    #11252620 - 10/15/09 01:58 PM (8 years, 2 months ago)

Just got the whole no scale so I just scaled it to see what it would be and its about 1/2 Tbs


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OfflineBrandNewbie
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Re: Need to expand my myc bank [Re: blujay]
    #11252643 - 10/15/09 02:01 PM (8 years, 2 months ago)

Quote:

blujay said:
Could I just hack a chunk off one of my cakes, put it in a jar of sterilized water, and break that up and then innoc the grains with that?




Take a colonized grain jar and use a syringe to add sterile water to it. Shake. Draw water into syringe. Use syringe to inoculate more jars.

It will probably work the way you are suggesting. Contamination rate would be high is my guess. :goodluck:


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Answer: Because they're ugly and they stink.


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Offlinecyb3rtr0n
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Re: Need to expand my myc bank [Re: blujay]
    #11252654 - 10/15/09 02:02 PM (8 years, 2 months ago)

yes you can make a LC out of a tissue sample.

there are many LC techniques out there. Karo Syrup is easy just like honey.  but karo light makes it easy to see the mycelium developing. 4% karo in water, steralise, etc..


here is another option:
http://www.shroomery.org/forums/showflat.php/Number/6590986#6590986


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Offlinecyb3rtr0n
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Re: Need to expand my myc bank [Re: Shea25]
    #11252666 - 10/15/09 02:04 PM (8 years, 2 months ago)

yea 1 teaspoon is about 5grams.  that is good for 100ml of water..

then pc is for about 30 minutes @ 15psi


Edited by cyb3rtr0n (10/15/09 02:06 PM)


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InvisibleShea25
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Re: Need to expand my myc bank [Re: blujay]
    #11252678 - 10/15/09 02:06 PM (8 years, 2 months ago)

Basically 100 mls of water to 1/2 tbs of Karo or honey. PC for 20 min let it cool and innoc with a tissue sample or MS syringe its up to you

Oh and when I scaled to see what 4 grams of karo was it was a 1/2 tbs right to the top of the measuring spoon


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Re: Need to expand my myc bank [Re: Shea25]
    #11252837 - 10/15/09 02:30 PM (8 years, 2 months ago)

5 ml = 1 teaspoon

1 teaspoon of karo or honey. i put that to 200 ml of water and, pc for 20 min at 15 psi to keep from carmelization. it works just fine for me.


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milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09


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Offlinemilkman59
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Re: Need to expand my myc bank [Re: milkman59]
    #11252869 - 10/15/09 02:34 PM (8 years, 2 months ago)

plus an easy way of cloning is to just pull the stem a part and put a chunk out the middle with sterile tweezers. make sure it not the out side of the stem.  drop into lc and be as sterile as can be.


--------------------
milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09


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Re: Need to expand my myc bank [Re: milkman59]
    #11252880 - 10/15/09 02:36 PM (8 years, 2 months ago)



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Offlineblujay
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Re: Need to expand my myc bank [Re: Shea25]
    #11259770 - 10/16/09 02:43 PM (8 years, 2 months ago)

Phew, that was fast guys!

I wanted to thank yall for this great info and your support. I've never seen a community this positive anywhere. You are all shining examples of what we need to be to get this illogical fear of substance forever liquidated.


--------------------

wat man rly


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Amazon Shop: ½ Pint Jars, Scales

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