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Offlineabesh
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Re: Cordyceps in Washington State? [Re: Roadkill]
    #7689877 - 11/28/07 10:52 AM (16 years, 2 months ago)

Cool. And like RK said thanks not only for spending money for testing and stuff, but also taking the time to share that info with us.
I have some questions, but I'll ask them in your thread in the cult forum.


Also how is the isolation on cricket agar coming along????


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If there are no stupid questions, then what kind of questions do stupid people ask? Do they get smart just in time to ask questions?
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InvisibleYidakiMan
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Re: Cordyceps in Washington State? [Re: abesh]
    #7689884 - 11/28/07 10:54 AM (16 years, 2 months ago)

How much did the metals test cost? I'd like to have tested some Chinese grown dried mushrooms.


Edited by YidakiMan (11/28/07 10:58 AM)


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: YidakiMan]
    #7690845 - 11/28/07 02:27 PM (16 years, 2 months ago)

$100. You can contact them directly at the address in the report above. I'll bet you a dollar the Chinese mushrooms have high levels of mercury.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleYidakiMan
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7691060 - 11/28/07 03:28 PM (16 years, 2 months ago)

That's a good bet to take.

What about sawdust pellets? My pellets say 100% Hardwood; no glues, accelerants
etc, but it also says not for use as a cooking fuel. What's keeping me from growing mushrooms on the pellets or moreso, whats keeping me from selling mushrooms grown on the pellets.


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: YidakiMan]
    #7691402 - 11/28/07 04:49 PM (16 years, 2 months ago)

Nothing. I've used pellets before. They expand like hell, so soak them first, then load into jars/bags after they separate back into regular sawdust. I'd supplement them with 10% to 20% wheat bran by volume, although you'd then have to sterilize. If they're non-treated pellets, I don't see why you can't call your mushrooms organic. Mushrooms grown on forest products are generally considered organic.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineChi Ro
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7713859 - 12/04/07 02:07 AM (16 years, 1 month ago)

The cricket agar is pretty genius. I must say.

Keep up the good work Roger. :thumbup:


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InvisibleHerbBaker
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7888338 - 01/16/08 01:51 PM (16 years, 16 days ago)

is there an update?


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Some things can grow without the light.--RJD


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OfflineJohn Smith
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Re: Cordyceps in Washington State? [Re: HerbBaker]
    #8214961 - 03/30/08 02:45 PM (15 years, 9 months ago)

am also pretty curious about this!


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I have no idea what I am talking about but I do know I say things you don't understand and if you do understand what I am saying then you are wrong.


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OfflineeVenom
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Re: Cordyceps in Washington State? [Re: John Smith]
    #8215286 - 03/30/08 03:55 PM (15 years, 9 months ago)

I don't know anything but I wouldn't have use cricket agar! since cordyceps only attacks a specific subspecies of animals.

So for example if it is a bee cordycep it wont affect a wasp.

Good luck!


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eVenom

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OfflineeVenom
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Re: Cordyceps in Washington State [Re: ohmatic]
    #8270557 - 04/11/08 05:29 PM (15 years, 9 months ago)

any Updates??


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
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InvisibleGGreatOne234
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #8270795 - 04/11/08 06:30 PM (15 years, 9 months ago)

Quote:

I also made up a batch of cricket agar to isolate the cordyceps farther.




:cool:


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InvisibleCracka_X
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #11210613 - 10/08/09 08:13 PM (14 years, 3 months ago)

I know I'd be reviving this post from the dead, but I thought your idea of making cricket agar was interesting.

Would it hurt if you made a mixture of insects into an agar solution so you wouldn't need to worry about the specificity of the cordycep strain you got?


--------------------
The best way to live
is to be like water
For water benefits all things
and goes against none of them
It provides for all people
and even cleanses those places
a man is loath to go
In this way it is just like Tao        ~Daodejing


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: Cracka_X]
    #11214370 - 10/09/09 10:27 AM (14 years, 3 months ago)

It doesn't seem that it would hurt.  Cordyceps don't naturally grow on crickets anyway.  I was living in a condo in Seattle at the time, and the pet store nearby had live crickets for sale.  I would speculate that other insects would also work well, but you'd just have to try and let us know.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineSuntzu
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #11243457 - 10/13/09 11:48 PM (14 years, 3 months ago)

Awesome work all around, RR.

I'm curious how the company generates species-specific results for consumers.  According to their site, they sequence a part of the ITS region, which is a very good place to start, but it's not clear whether they utilize NCBI or their own internal database for phylogenetic construction and/or species identification.  The sample report I saw just seemed to kick out 'the closest match'.  If they are only using an in-house database I don't know that I'd trust their species identification of a 'fungi perfecti'.  Most of their business appears to be directed toward environmental isolates and their in house library may not include m/any Cordyceps spp.  Odds are the results are valid, I'm just playing devil's advocate here.  Since they are just an identification service, I seriously doubt they'd do this for you, but for a hundred bones I personally would really like to get a hold of the actual sequence data they generated.  It is a very simple thing to plug that data into NCBI and see what matches pop up there when you blast it [and how good those matches are].  I guess the main point is that if their library doesn't include at least some closely related Cordys, you're going to get 'garbage out' in that report they send you [even if it was an ATCC Cordyceps you submitted]


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: Suntzu]
    #11246035 - 10/14/09 01:25 PM (14 years, 3 months ago)

It was John Holliday of alohamedicinals.com who actually sent the mycelium to the DNA lab, after I sent it to him.  I'm assuming they're someone he works with on cordyceps a lot, so they have his cultures to sequence.  I don't have any of the paperwork other than what's scanned above.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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