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OfflineGrzyby
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cloning wild shaggy mane
    #11242237 - 10/13/09 10:20 PM (8 years, 1 month ago)

I think i found a nice patch of Coprinus comatus with about 5 nice sized clumps. One clump was older and was turning into black goo. Are there any other similar species that also rot into black goo? Going to go back tomorrow and get a few younger ones to clone. I also plan on digging some soil up from the area and mixing it with compost to try to make a bed. Any advice for cloning wild mushrooms? I had success with cloned indoor specimens but failed with some outdoor wine caps.


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Offlinecharvo
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Re: cloning wild shaggy mane [Re: Grzyby]
    #11242391 - 10/13/09 10:41 PM (8 years, 1 month ago)

cloning outdoor mushrooms can be tricky. I'd add h2o2 to your agar, and an antibacterial if you have any. Other wise you may just have to isolate away from the contaminated parts.

As far as a positive identification on the comtus, it is one of the easiest species to identify. there is another coprinus( ink cap)i believe. you should look this one up. my field guild is in the garage at the moment. So i'm going by memory, double check me on that one. This mushroom also quickly turns into a pile of black shit.

It doesn't look like the shaggy at all thou.


here is a picture of the shaggy mane's that i clone a few years back.


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OfflineGrzyby
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Re: cloning wild shaggy mane [Re: charvo]
    #11242941 - 10/14/09 12:02 AM (8 years, 1 month ago)

Thanks, i will post some pix when i get them and look into adding h202 to my agar. I think its probably to late as the agar is already cooling now.


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Offlinecharvo
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Re: cloning wild shaggy mane [Re: Grzyby]
    #11243055 - 10/14/09 12:19 AM (8 years, 1 month ago)

you need to add the h202 when the agar is at 125 degree f. maybe you'll read this in time enough to get it in there

.5ml:150ml agar. should do it.

good luck either way.


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Edited by charvo (10/14/09 12:32 AM)


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OfflineMycelio
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Re: cloning wild shaggy mane [Re: Grzyby]
    #11244265 - 10/14/09 07:26 AM (8 years, 1 month ago)

I'd also use stem buds for the outdoor bed and mix in some wood chips.

For indoor cloning, in parallel to the agar plates, put some pieces from stem buds on corrugated cardboard, mycelium will grow fine while bacteria and molds won't.

Second image shows stem buds in straw, but cardboard is easier. You may also create cardboard spawn to inoculate outdoor beds.

Carsten


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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