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OfflineBreakfast Crew
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Inoculation Glass side vs middle.
    #11239910 - 10/13/09 04:35 PM (8 years, 2 months ago)

I just wanted to know why people prefer to inoculate along the glass side of a their jars rather than the middle. Is it for visual purposes? Lets say I decided to use one injection site in the center. Would it be 100% safe to assume after full colonization from injection in the middle that it had no contamination in the center?

I think just a tiny squirt is enough, I don't want to hear anyone telling me to use half a syringe to watch the water drip down the side.


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InvisibleSlimz
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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240012 - 10/13/09 04:49 PM (8 years, 2 months ago)

the reason we inoc from the side is so we can SEE contam as soon as possible.

inoc in the center will grow just fine.. but the answer is NO
just because it all looks good does not bean that there is not a patch of trich in the center..


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Re: Inoculation Glass side vs middle. [Re: Slimz]
    #11240052 - 10/13/09 04:56 PM (8 years, 2 months ago)

what he said^

:scaryshroom::thumbup:


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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240139 - 10/13/09 05:07 PM (8 years, 2 months ago)

I inoculated the centre of a 1L WBS jar once just to see how it would work.

The one thing that stopped me from doing it again was a big ball of mycelium grew in the centre which I couldn't see until it reached the sides. It literally grew from the centre out to the glass pretty evenly, filling the jar, and making it impossible to shake apart.

The only way you're going to be able to tell when your jars are 30% colonized is if you inoculated at the glass.


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OfflineBreakfast Crew
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Re: Inoculation Glass side vs middle. [Re: Slimz]
    #11240419 - 10/13/09 05:55 PM (8 years, 2 months ago)

Quote:

Slimz said:
the reason we inoc from the side is so we can SEE contam as soon as possible.

inoc in the center will grow just fine.. but the answer is NO
just because it all looks good does not bean that there is not a patch of trich in the center..




What is the reason of spotting contaminates as soon as possible?  I'm not working a on a time schedule here and everything done is pretty much long term. And as far as I know, you would still have no idea if there were contaminates in the jar until you have opened them. Unless you see healthy grow from the center, yes/no? that was my question.


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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240432 - 10/13/09 05:58 PM (8 years, 2 months ago)

well then the answer remains the same..


you can see healthy grow around the entire cake, and STILL break it open to find contam (like trich)

WORST part is that you wont know till you break it open and spread green spores EVERYWHERE


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Offlinenexus1946
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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240455 - 10/13/09 06:03 PM (8 years, 2 months ago)

Quote:

Breakfast Crew said:
Quote:

Slimz said:
the reason we inoc from the side is so we can SEE contam as soon as possible.

inoc in the center will grow just fine.. but the answer is NO
just because it all looks good does not bean that there is not a patch of trich in the center..




What is the reason of spotting contaminates as soon as possible?  I'm not working a on a time schedule here and everything done is pretty much long term. And as far as I know, you would still have no idea if there were contaminates in the jar until you have opened them. Unless you see healthy grow from the center, yes/no? that was my question.




If you spot contamination early you can toss the jar and start over if need be. Saves time.

Also, if you're unfortunate to have a contaminated syringe you will see it early at the glass rather than in the centre of the jar where it could remain hidden longer.


--------------------
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Re: Inoculation Glass side vs middle. [Re: Slimz]
    #11240456 - 10/13/09 06:03 PM (8 years, 2 months ago)

some of my pf jars have 3 holes for against the glass inoc and one in the middle


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OfflineBreakfast Crew
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Re: Inoculation Glass side vs middle. [Re: Slimz]
    #11240509 - 10/13/09 06:13 PM (8 years, 2 months ago)

http://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture

There is a picture near the bottom that explains my interest in this topic.  He cuts a cake open and WHAM, the mean green machine. Notice more white around then in the middle.  Honestly, do you not believe inoculating from the inside proves "better safer than sorry" in the long run?


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Re: Inoculation Glass side vs middle. [Re: nastos]
    #11240516 - 10/13/09 06:15 PM (8 years, 2 months ago)

if all else fails refer to the "hydra tek"


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Offlinenexus1946
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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240564 - 10/13/09 06:23 PM (8 years, 2 months ago)

Quote:

Breakfast Crew said:
http://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture

There is a picture near the bottom that explains my interest in this topic.  He cuts a cake open and WHAM, the mean green machine. Notice more white around then in the middle.  Honestly, do you not believe inoculating from the inside proves "better safer than sorry" in the long run?




No.

If your tek is good and sterile then, other than a contaminated syringe, you should be fine.

I take it your talkin' BRF cakes and not WBS so you're OK to inoculate in the centre as well; providing you trust your syringe.

For WBS I'd advise against it.


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Offlinenexus1946
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Re: Inoculation Glass side vs middle. [Re: cloudsaregathering]
    #11240573 - 10/13/09 06:24 PM (8 years, 2 months ago)

Quote:

cloudsaregathering said:
if all else fails refer to the "hydra tek"




Oh...not that again! :facepalm:


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OfflineBreakfast Crew
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Re: Inoculation Glass side vs middle. [Re: Slimz]
    #11240602 - 10/13/09 06:29 PM (8 years, 2 months ago)

Right, I wouldn't bother with asking when dealing with WBS. Me and Mr.Clean have issues so it brings me to my point again, (I'd expect the same from other beginners) I still don't have an answer.

and cloud, don't think that crap into my thread.


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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240753 - 10/13/09 06:57 PM (8 years, 2 months ago)

glass beats middle.


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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240817 - 10/13/09 07:08 PM (8 years, 2 months ago)

Quote:

Breakfast Crew said:
I still don't have an answer.
Quote:



I gave you an answer. Well, my opinion anyway.

I said "no". "If your tek is good and sterile then, other than a contaminated syringe, you should be fine."

In other words, I wouldn't worry too much about it.

It not going to hurt it any if you do decide inoculate in the centre as well. Just remember, the more inoculation points you have, the more moisture you're adding, unless you 'hold back' a little on the syringe. This of course is assuming you're not only inoculating in the centre.


--------------------
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The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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Re: Inoculation Glass side vs middle. [Re: Breakfast Crew]
    #11240950 - 10/13/09 07:33 PM (8 years, 2 months ago)

I use only one hole in the middle to inoculate my 1/2 pints.

I use a millet/verm mix and with 1/4cc or less of spore fluid in one hole in the center, the cake is done in about 14-21 days.

I have couple of lids with holes in the edge, so whenever I make a batch of new syringes, I use the side hole for reasons already described in this thread(I wanna see contams if there are any).

I think that the reason that folks inoculate on the side is that when you inoculate directly into the substrate the inoculate does not run as far as it would if it could run down the glass.

So inoculating against the glass is probably the best way to go in most cases, but I like the one hole in the middle.

As far as the nasties(contamination) go, you can never be sure that there are none unless there is none.

:vaped:


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