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OfflineJackal
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Agar Overflow
    #1123899 - 12/09/02 04:15 AM (18 years, 7 months ago)

I've tried to do a search but couldn't find the post I'm referring to. I am about to have my first venture into using agar. I only have a 250ml flask, and someone mentioned that the agar solution rises during sterilization. How much can I fill the flask - 1/2, 3/4 full???

thnx


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Offlinezeronio
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Re: Agar Overflow [Re: Jackal]
    #1123975 - 12/09/02 05:17 AM (18 years, 7 months ago)

You can fill it up, just close the flask with tightly packed polyfill.
I sterilize my agar in a normal jar filled up to 80%, covered with Tyvek filter and lid with a hole.


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OfflineAnnoA
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Re: Agar Overflow [Re: Jackal]
    #1124013 - 12/09/02 06:16 AM (18 years, 7 months ago)

Some, thicker, agar formulations tend to boil over when cooling down.

To make sure you shouldn?t fill the flask more than 1/2 full.


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OnlineDERRAYLD
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Re: Agar Overflow [Re: Anno]
    #1124017 - 12/09/02 06:19 AM (18 years, 7 months ago)

Or let it cool slowly


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OfflineAnnoA
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Re: Agar Overflow [Re: DERRAYLD]
    #1124026 - 12/09/02 06:47 AM (18 years, 7 months ago)

Sure, I allways let it cool slowly in the pressure cooker, but with some agars it just boils over, no matter what i attempt...


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OnlineDERRAYLD
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Re: Agar Overflow [Re: Anno]
    #1124031 - 12/09/02 06:53 AM (18 years, 7 months ago)

I always only fill the agar to 3/4 of the jar as you suggested anyway Anno.
SO I think that is the best solution in this case.


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