Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Cultivation Supplies

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Agar questions
    #11236776 -

hey all i recently started my agar project and i had a few questions :

1. when you inoculate with a syringe do you just need a drip in a corner of plate( i got 3 section dishes ). when i knocked one up i squirted a lil bit of lc on it and the liquid ran across the whole section . or do i need to actually puncture the agar with syringe and inject into the agar?

2. how many dif types of inoculations is there for agar ?

3 . when done i just take micro pore all around dish label which section is which and throw it in same type incubator as jars ? ( i use a cardboard box with a heating pad under it .

4. is it better to pour agar thin ?

5. can i just clean used agar plates and put them in dish washer and wipe with iso when i am getting ready to pour again ?

6. i should be able to do agar fine in a glove box or do i need a flow hood ? is a flow hood better to have in general rather than glove box ?

7. if a plate becomes contaminated on one part in a 3 section dish is it very possible the other two parts will too ? if i get a dish contam can i just cut out a good clean looking piece of myc and do a transfer ?

i know some of these might sound stupid but i just want to be sure i looked up a few diff things i asked but didn't get a real exact answer .

anyone got any videos or pics of their inoculation process i would be glad to look at them . Ive seen rr videos they cover good amount of things very well


--------------------
Super Easy LC

Shroomerys secrets to free things !! All users contribute!!

The reason my post count is so low is because I know how to use the  search option on this site

Rotten Status : closed site gone

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11236805 -

1. try to drip the liquid in the middle. dont puncture
2. spore print, spore solution, lc, agar transfer, tissue transfer im sure the list goes on
3. sounds good
4. I dont know tend to pour heavy personally
5. You will probaly get lots of contaims throw the plate.
6.It's better with a flow hood but a GB works it can just be tricky sometimes
7. Yes just transfer to a new plate


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11236824 -

1) a drip- but in the center of a section, not a corner
2) spores, live mycelium, tissue, LC... at least those
3) Yep. You can put each one in a clean baggie if you like. Try not to move them or them or breathe on them too much.
4) dunno
5) Sterilize them.
6) Glove box at minimum, it'll work ok but you'll have some contams. You'll get better with practice, it's as much about technique as it is about equipment.
7) Likely- transfer healthy cultures away from contams ASAP.

edit- I had 3 and 4 switched.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: Agar questions [Re: Doc_T]
    #11236918 -

I was kinda wondering about that doc.  before the edit.  I am pleased my answers were pretty close to yours but it seems you reuse plates


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11244193 -

I also just started working with agar. Poured 20 dishes today and have a stack of dishes with lots of condensation in them. I think I didn't let the agar cool long enough after taking out of the pressure cooker (let it sit in PC for two hours). I also think I poured them a little thick which could've led to higher internal temps.  I say this as a point of caution when you pour, but I'd also like to know from the pros out there what I should do with these dishes. They were carefully covered with the original sleeve while still in the glovebox and 12hrs later condensation is the same. I'm concerned that the moisture will invite contams so...

Should I wait until it clears?
Noc them up as they are without worrying?
Toss them and start anew knowing that contams are very likely?

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11244617 -

truskool said:
I am pleased my answers were pretty close to yours but it seems you reuse plates


I bought glass dishes. :shrug: I'm not really a 'disposables' kind of guy.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: Agar questions [Re: Doc_T]
    #11244643 -

I squirted some LC on a dish.. of course it was no drip.. it was a squirt. The dish is a complete ugly mess of myc, but no contams :smile:

I will now be careful just to make a drop in the future..


--------------------

Extras: Filter Print Post Top
Re: Agar questions [Re: Herbal_Elixer]
    #11246405 -

mush mike i had condensation on some of mine as well but it seems that most say to pour at lower temps otherwise let the agar cool down alot then pour dishes .

they should be fine


--------------------
Super Easy LC

Shroomerys secrets to free things !! All users contribute!!

The reason my post count is so low is because I know how to use the  search option on this site

Rotten Status : closed site gone

Extras: Filter Print Post Top
Re: Agar questions [Re: Doc_T]
    #11246657 -

Doc_T said:
truskool said:
I am pleased my answers were pretty close to yours but it seems you reuse plates


I bought glass dishes. :shrug: I'm not really a 'disposables' kind of guy.


you and me both!

Glass beats plastic no matter what any one says.

Extras: Filter Print Post Top
Re: Agar questions [Re: badman]
    #11246828 -

were would i get additional petris in my local area ?


--------------------
Super Easy LC

Shroomerys secrets to free things !! All users contribute!!

The reason my post count is so low is because I know how to use the  search option on this site

Rotten Status : closed site gone

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11246838 -

any science shops around? hobby stores


--------------------

Pf tek done right
How to Sterilize a Syringe
Quick and Easy Pasteurization
Quick and Easy LC
Pan Cyans without a PC
4 Easy step rye

AMU Q&A link

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11246844 -

plastic or glass?
Plastic at certain hobby stores
Online


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11246881 -

Fungifarmer has some good prices on petri dishes, i think there a sponser...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11246888 -

He;s asking where in his local area.

Walk around the town look for science shops hobby store I was amazed how many shops carried stuff for this kinda work in my small town


--------------------

Pf tek done right
How to Sterilize a Syringe
Quick and Easy Pasteurization
Quick and Easy LC
Pan Cyans without a PC
4 Easy step rye

AMU Q&A link

Edited by Shea25 (10/14/09 03:41 PM)

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11246908 -

cloudsaregathering said:
Fungifarmer has some good prices on petri dishes, i think there a sponser...


Nope there not


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247072 -

cloudsaregathering said:
Fungifarmer has some good prices on petri dishes, i think there a sponser...


thats who i use and family members get discounts


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Re: Agar questions [Re: Shea25]
    #11247139 -

hmm okie sounds good i think we got a discovery channel store in our mall ill check there


--------------------
Super Easy LC

Shroomerys secrets to free things !! All users contribute!!

The reason my post count is so low is because I know how to use the  search option on this site

Rotten Status : closed site gone

Extras: Filter Print Post Top
Re: Agar questions [Re: feelfunny]
    #11247146 -

feelfunny said:
cloudsaregathering said:
Fungifarmer has some good prices on petri dishes, i think there a sponser...


thats who i use and family members get discounts


discount what, wish i would get an invite to the Family...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: nickelz34]
    #11247147 -

i have seen them there but they ream you on he price. like 2 for 5$ or 10 for like 15


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Re: Agar questions [Re: feelfunny]
    #11247232 -

for the plastic 2 section plates says 20 for $7.00...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247240 -

yea so thats 40 dishes


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247248 -

I may be wrong but i think they are stacked one on top of another


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11247275 -

says 20 per sleeve...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247307 -

Yea fuck your right that sux.  Ill know for sure in a couple of days


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11247334 -

I buy online..

DISPOSABLE PETRI DISH, 15x100mm, STACKABLE, 25/PK  3.95

DISPOSABLE COMPACT PETRI DISH, 15x60mm, STACKABLE, 20/PK 3.00

DISPOSABLE PETRI DISH, 20x100mm, NON-STACKABLE, 20/PK  3.50


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247354 -

on line  not in most stores. i know the teacher stores chage 9$ for 3


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Re: Agar questions [Re: feelfunny]
    #11247360 -

GLASS PETRI DISH, 60x15mm,10/PK 7.00

GLASS PETRI DISH, 75x15mm,10/PK 7.25


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247361 -

Damion5050 said:
I buy online..

DISPOSABLE PETRI DISH, 15x100mm, STACKABLE, 25/PK  3.95

DISPOSABLE COMPACT PETRI DISH, 15x60mm, STACKABLE, 20/PK 3.00

DISPOSABLE PETRI DISH, 20x100mm, NON-STACKABLE, 20/PK  3.50


Could I get a link for that?


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11247372 -

http://cynmar.com/SearchByCategory.aspx?CategoryCode=331


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247422 -

awesome...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247430 -

Also they get cheaper the more you buy if you buy at least 500 of those 3.95 ones the price drops to 3.50 per pack and the 3.00 ones go down to 2.70 if you buy at least 500


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247495 -

wow now thats a deal...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247660 -

Damion5050 said:
Also they get cheaper the more you buy if you buy at least 500 of those 3.95 ones the price drops to 3.50 per pack and the 3.00 ones go down to 2.70 if you buy at least 500


why does FireFox keep telling me the site has invalid info...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247661 -

it worked for me


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Re: Agar questions [Re: feelfunny]
    #11247674 -

did you try to check out?


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247740 -

I use firefox and it works for me as well.. Did you sign up for an account so to speak ??  Also you can call them they are really friendly I had a question about some of there products.. So if need be u can phone in your order.


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247827 -

mabye my security settings are set too high... yeah i signed up for an account when i go to check out firefox freaks out...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11247898 -

cloudsaregathering said:
mabye my security settings are set too high... yeah i signed up for an account when i go to check out firefox freaks out...



Huh I have ordered 3 or 4 times from them with no prob.. Like I said if need be call them up or try using IE


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11247920 -

yeah will do them some good prices...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11248283 -

They are good IME


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Agar questions [Re: truskool]
    #11248560 -

damn just tried with IE and wont even let me add to my shopping cart...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11248571 -

:sad:


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Re: Agar questions [Re: feelfunny]
    #11248584 -

yeah i don't know whats up i'd like to get some of those dishes...


--------------------

"the root of the problem has been isolated"

Extras: Filter Print Post Top
Re: Agar questions [Re: cloudsaregathering]
    #11248959 -

Not sure what the deal is.. I just did it. I went all the way to the last step where you have to confirm your order without any problems..

Try deleting your cookies.


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11248980 -

When I click checkout everything worked fine.. When I installed a new firefox update I had to download the security certificate for the site again.. Does anything come up about add security extension.


--------------------
AMU Q&A:plot:
AMU Genetic Library:stirthepot:
My Elementary Coir Tek
My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


Extras: Filter Print Post Top
Re: Agar questions [Re: Damion5050]
    #11249019 -

I used firefox last week to order with NP's


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Agar question sill_o_cin 4,236 4 11/21/01 05:23 PM
by sill_o_cin
* Agar Questions cantara 5,736 6 08/09/01 06:22 AM
by ar393
* might be an advance agar question
( 1 2 all )		 fugu 11,617 33 02/05/03 06:45 AM
by fugu
* agar question mycocitizen 1,334 1 11/14/01 11:20 PM
by sylo
* Agar questions-I may have goofed blahblahblah 3,636 8 07/22/25 07:32 PM
by Brainless Goblin
* Re: Attn: ShroomGod, agar questions vivid 2,979 9 01/12/00 03:49 PM
by Anonymous
* Pan.cyan mycelium on agar questions.... mesq 2,565 1 05/08/03 12:59 AM
by zeronio
* handle agar question fugu 1,931 10 02/12/03 08:11 AM
by tripndicular

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,439 topic views. 15 members, 1,163 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.053 seconds spending 0.015 seconds on 16 queries.