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koopa_troopa said:
I'm sure many of the experienced members around here have heard of supplementation at casing aka SACing, yet I have never seen or heard of anyone on these forums actually putting this much tested and seemingly amazing technique to work. This baffles me, seeing as how there are hundreds of threads on these boards discussing ways to increase flushes and yield, yet here lies one of the must proven and easiest methods to do so and no one does it.

For those that are unaware what SACing is, it is the practice of breaking apart colonized bulk substrates and adding supplementing it with organic material usually rich in nitrogen and lipid content. Common materials usually consist of ground soybean, flax seed, and rape seed.

I believe the lack of SACing attempts by members is due to the fear of shredding precious bulk subs which are colonized and ready to fruit & I believe I may have stumbled upon a method to potentially bypass this issue.

I just ordered one of these solid flavor injectors http://www.tvtopten.com/solid-flavor-injector.html which more or less acts as a syringe that is capable of injecting solid material. I will be using it to inject pasteurized ground soybean periodically throughout my next colonized substrate and will compare the results with an identical substrate colonized by the same cube isolate. Sounds like a damn fine evening of experimentation if you ask me..

Any comments.. suggestions!? And please.. I would love to hear if anyone has any experience with either SACing or SASing, im all ears.. I myself have tried it recently and had great results but unfortunately did not have a control so it may have been shear luck (or just mad skizillz!!) that I had such killer yeilds. But frankly, Im dumfounded no one around here has tried a technique that consistently proves to increase yields by 80-150%!!

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  Schisler and Sinden (6) reported a beneficial effect of supplementing mushroom compost with certain refined and crude vegetable oils at casing. The treatment was reported to increase mushroom yield, particularly in the first break or flush of mushrooms. This suggested a relationship between lipid metabolism and the initiation of fruiting in the cultivated mushroom. They used a compost consisting of either synthetic compost or a mixture of synthetic and straw-bedded horse manure. The research reported in this paper is a continuation of this approach to compost supplementation. Various lipids and lipid-protein combinations were tested for their effect on yield of the cultivated mushroom, Agaricus bisporus (Lange) Sing., in an attempt to determine which component(s) in the oils was responsible for the fruiting stimulation. Whether the compost to which the oils were added had an effect upon the mushroom yield response was also of interest. In the present investigation, conducted at the Mushroom Research Center of The Pennsylvania State University, University Park, the compost consisted of straw-bedded horse manure.

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TABLE 1. Effect of ground soybeans and cottonseed
oil supplemented at spawning

Treatment(a)                          Yield (lb/fPt')(b)
Normal spawn rate                                    .................2.01
Normal spawn rate plus ground soybeans    (400 g) .................... 2.09
Normal spawn rate plus cottonseed oil    (250 ml) .................... 2.11
Four times normal spawn rate                                    ...... 2.47
Four times normal spawn rate plus ground soybeans (400 g)    ..........2.93 *
Four times normal spawn rate plus cottonseed oil (250 ml) ............ 3.11

(a) Normal spawn rate was 110 g per tray. Amounts of supplements given represent amount per tray.
(b) Least significant difference, 0.05 = 0.40 lb/ft2.

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PassiveAgressive said:
(Stimulation of Yield in the Cultivated Mushroom by Vegetable Oils' by LEE C. SCHISLER)

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Supplement                Amnt. added per tray              Yield (lb/ft2)
None.......................                                      3.72
Cottonseed meal.............. 250 g                              3.91
Cottonseed meal.............. 500 g                              4.48
GB-18b ................      186 g                              4.05
GB-18 ......... .......      372 g                              3.43
GB-18................        558 g                              2.33
Cottonseed meats(b) .......  400 g                              4.52
Cottonseed meats............. 800 g                              2.75
Ground soybeans ............. 286 g                              4.48
Ground soybeans.............  572 g                              4.36
Crude cottonseed oil (Proflo)(b). 200 ml                          4.48
Crude cottonseed oil (Proflo) 400 ml                              4.42
Refined cottonseed oil .......200 ml                              4.58
Refined cottonseed oil ...... 400 ml                              4.88
Refined soybean oil ......... 200 ml                              4.50
Refined soybean oil.......... 400 ml                              4.58

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**Sorry about the quality. The quotes were grafted from a .pdf **
There sure does seem to be some fairly good looking data here! If what im reading is true then it's been overlooked for way to long.

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ratdog said:
so are you saying in the last 2 posts that at 100% colonization of a substrate by a high spawn rate with the addition of oil by lets say injection into said preparation will work.?

spawn sub wait for 100%
inject oil
get more shrooms

or something like that.

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gngsteroflove said:
I can't believe that this topic has come up again.
I currently have two shoe boxes going for a comparison.
Both are the same spawn. Both are the same substrate mix.
The only difference is that one of them has toasted, ground soy in it.
At last look, they were both at about the same.
The sub is a basic CCV with pelletized gypsum added in the mix.
One has soy meal. The other doesn't.
I'm waiting on full colonization and will subject them to the same rigorous fruiting conditions.
this is funny.
soybeans, who knew?
or not?
IDK

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koopa_troopa said:
stay tuned pics soon!

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stonesun said:

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koopa_troopa said:
stay tuned pics soon!

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anonjon said:

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gngsteroflove said:
I can't believe that this topic has come up again.
I currently have two shoe boxes going for a comparison.
Both are the same spawn. Both are the same substrate mix.
The only difference is that one of them has toasted, ground soy in it.
At last look, they were both at about the same.
The sub is a basic CCV with pelletized gypsum added in the mix.
One has soy meal. The other doesn't.
I'm waiting on full colonization and will subject them to the same rigorous fruiting conditions.
this is funny.
soybeans, who knew?
or not?
IDK

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I think you're missing the point entirely. The idea is to supplement at precise times, not just tossing it into the sub.

That's not to say that your method won't serve as a control for comparing supplementation at spawning against casing.

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PassiveAgressive said:

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ratdog said:
so are you saying in the last 2 posts that at 100% colonization of a substrate by a high spawn rate with the addition of oil by lets say injection into said preparation will work.?

spawn sub wait for 100%
inject oil
get more shrooms

or something like that.

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I wish this was my data, but it's not. I haven't tried this myself but from what I understand, yes, a high spawn ratio with injections of refined soy-bean oil (most veggie oils in my area are comprised mostly of soy) into the fully colonized substrate.

Very convincing sources and data. Nothing to shake a stick at. It amazes me that the Shroomery didn't catch this until now!

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