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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Simple agar question
#11223293 - 10/10/09 09:08 PM (14 years, 5 months ago) |
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ok i stuck a piece of MYC from a grain on an agar plate..
2 days later it shot out an arm like a ryzo, so i cut it off and put it on a different plate (looking to sterilize the sample more) is that OK..
i have been growing for a while but agar is new to me..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Simple agar question [Re: Slimz]
#11223312 - 10/10/09 09:12 PM (14 years, 5 months ago) |
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What are you asking?
-------------------- You make it all possible. Doesn't it feel good?
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ratdog
wild capture trader
Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Simple agar question [Re: Slimz]
#11223316 - 10/10/09 09:13 PM (14 years, 5 months ago) |
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Quote:
Slimz said: ok i stuck a piece of MYC from a grain on an agar plate..
2 days later it shot out an arm like a rizo, so i cut it off and put it on a different plate (looking to sterilize the sample more) is that OK..
i have been growing for a while but agar is new to me..
in a g box or in front of a flow hood you will be golden man.
cleaning up rizo is easy gust transfer transfer etc.
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Simple agar question [Re: Doc_T]
#11223320 - 10/10/09 09:13 PM (14 years, 5 months ago) |
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is it kk to chop a piece of the ryzo looking growth off and transfer it to a new dish.. will it continue to grow (im assuming yes) or do i have to wait for it to fan out?
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Simple agar question [Re: Slimz]
#11223330 - 10/10/09 09:15 PM (14 years, 5 months ago) |
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Quote:
Slimz said: is it kk to chop a piece of the ryzo looking growth off and transfer it to a new dish.. will it continue to grow (im assuming yes) or do i have to wait for it to fan out?
Oh. It'll grow. You might be better off waiting to see if it's going to sector, though. If you don't it just has to sector out on the new dish.
-------------------- You make it all possible. Doesn't it feel good?
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Simple agar question [Re: Doc_T]
#11223342 - 10/10/09 09:18 PM (14 years, 5 months ago) |
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ok.. ill let this sector.. i was just trying to sterilize it a bit.. i came from an open air grain so i was trying to eliminate contam from the original sample..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Simple agar question [Re: Slimz]
#11223355 - 10/10/09 09:21 PM (14 years, 5 months ago) |
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Oh! Good thinking. Transfer healthy myc away from contams ASAP.
-------------------- You make it all possible. Doesn't it feel good?
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Simple agar question [Re: Doc_T]
#11223370 - 10/10/09 09:24 PM (14 years, 5 months ago) |
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ok .. thought i had correct thinking, but im new to agar so i was checking..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Simple agar question [Re: Doc_T]
#11223376 - 10/10/09 09:25 PM (14 years, 5 months ago) |
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Yes if there is visible contamination transfer healthy tissue away from it.. But if there is no such things watch for some sectors..
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Simple agar question [Re: Damion5050]
#11223446 - 10/10/09 09:37 PM (14 years, 5 months ago) |
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awesome thnx
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
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Re: Simple agar question [Re: Slimz]
#11223825 - 10/10/09 10:38 PM (14 years, 5 months ago) |
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damn im late
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
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Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Simple agar question [Re: feelfunny]
#11223842 - 10/10/09 10:42 PM (14 years, 5 months ago) |
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but you tried.. its the thought that counts
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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Jitsu
JKD Love
Registered: 11/12/08
Posts: 1,073
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Re: Simple agar question [Re: Slimz]
#11223947 - 10/10/09 10:57 PM (14 years, 5 months ago) |
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The primary reason we use agar is to isolate a culture away from contaminants. I doubt you will have trouble isolating Cubensis Mycelium away from contamination.
Your agar medium should be sterile and kept that way until you inoculate with said culture/spores. Don't open your dishes unless you are going to make transfers. If you don't have a Flowhood a Glovebox works fine, just very space restricting.
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
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