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OfflineCJCollin37
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Registered: 08/10/09
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What should i do???
    #11220811 - 10/10/09 03:19 PM (8 years, 13 days ago)

So the cambo syringe that i ordered months ago via usps thru spores101 just came today. Since I ordered that one, I have already began a grow with a another cambo syringe from spores101 and my jars have been colonizing for a few weeks now.

Should I just hang onto this new syringe? I was thinkin about inoculating my already colonizing jars with it to speed up the process cuz its taking forever and im gettin impatient. But i dont wanna risk contams. I was also thinkin when my jars are 100% and i spawn to bulk, I could use the syringe to inoculate the bulk sub and speed that process up.

I don't see much use in saving it for the next grow cuz I already have a Brazilian print and I plan on using an isolate for an lc once these babies fruit...

So I guess my question is, what would you do??
Opinions?

(p.s. i'm a newb; first grow)


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InvisibleSlimz
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Re: What should i do??? [Re: CJCollin37]
    #11220821 - 10/10/09 03:21 PM (8 years, 13 days ago)

NOOO.. do not add more spores.. that makes things slower because of competing genetics.

if you have no use for it, give it away..

also you should not use sponsors names when talking about ordering spores then growing them out.. just say "a sponsor"


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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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OfflineCJCollin37
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Re: What should i do??? [Re: Slimz]
    #11220829 - 10/10/09 03:23 PM (8 years, 13 days ago)

Oh, shit. Thanks for the heads up.
Would they still be competing if I inoculated substrate with crumbled cake in it?


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InvisibleSlimz
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Re: What should i do??? [Re: CJCollin37]
    #11220842 - 10/10/09 03:24 PM (8 years, 13 days ago)

why would you put spores in a bulk sub that already has a crumbled cake in it?

dont do that, its silly...


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: What should i do??? [Re: Slimz]
    #11220855 - 10/10/09 03:28 PM (8 years, 13 days ago)

Plus why would you mix spores from them with your good ones? :confused:


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OfflineCJCollin37
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Re: What should i do??? [Re: Slimz]
    #11220869 - 10/10/09 03:30 PM (8 years, 13 days ago)

Idk, for speed? I guess ur right. I just wanted fruits by thanksgiving break, my bro's comin home and i wanted to surprise him.

I guess it was a dumb question.. i'll leave it be


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InvisibleSlimz
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Re: What should i do??? [Re: CJCollin37]
    #11220876 - 10/10/09 03:32 PM (8 years, 13 days ago)

dont leave it be.. let it be :smile:


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Offlinefundamentalchair
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Re: What should i do??? [Re: Slimz]
    #11220963 - 10/10/09 03:51 PM (8 years, 13 days ago)

Spores won't germinate on most bulk substrates. Nock up some new jars or start some LC.

Why are you waiting for a fruit to start an isolate? Just take the spores from the new syringe and dump it on some agar. Start isolating now, its a long and tedious process.


--------------------
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OfflineCJCollin37
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Re: What should i do??? [Re: fundamentalchair]
    #11221008 - 10/10/09 04:02 PM (8 years, 13 days ago)

Quote:

fundamentalchair said:
Spores won't germinate on most bulk substrates. Nock up some new jars or start some LC.

Why are you waiting for a fruit to start an isolate? Just take the spores from the new syringe and dump it on some agar. Start isolating now, its a long and tedious process.




Oh? I was just gonna take a chunk of my best lookin, fastest shroom and put it in an LC..


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InvisibleSlimz
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Re: What should i do??? [Re: CJCollin37]
    #11221022 - 10/10/09 04:05 PM (8 years, 13 days ago)

thats a good idea too, but LCs contam easily, agar is the way to go..

when you toss some of the big fruit in the LC you may also toss in a few spores so even if it does not contam, its not isolated..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Offlinefundamentalchair
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Re: What should i do??? [Re: CJCollin37]
    #11221038 - 10/10/09 04:09 PM (8 years, 13 days ago)




Oh? I was just gonna take a chunk of my best lookin, fastest shroom and put it in an LC..




That would be cloning, and not isolation. While beneficial, they are not the same thing.

The basics of isolation are placing spores (or a clone tissue sample) on an agar dish. from this, it will grow into 'sectors', or different genetic patterns. one then takes a portion of each of the sectors and moves it to another plate. Keep this process up until the new plates no longer sector. This can cause 30+ isolates. Each isolate is then grown and fruited to see which one performs the best. The best performer(s) are then moved from the agar to a master agar slant and usually into LC.

Further isolates can be made from the spores dropped from an isolate, genetically disposing future generations for certain traits.

Bet you didn't know you could do genetic engineering at your kitchen table, did ya?


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light


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InvisibleSlimz
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Re: What should i do??? [Re: fundamentalchair]
    #11221047 - 10/10/09 04:12 PM (8 years, 13 days ago)



--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCJCollin37
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Registered: 08/10/09
Posts: 592
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Re: What should i do??? [Re: Slimz]
    #11221122 - 10/10/09 04:26 PM (8 years, 13 days ago)

:shocked: I guess I have a lot more reading to do. I didn't realize what an isolate was...

It seems like a clone would be an isolate...? I thought a single shroom is the product of a substrain that finally isolated itself genetically. So if you clone it, aren't you isolating that fruit and those genetics?

Thanks for the help guys (and your patience :tongue:)


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OfflineCJCollin37
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Re: What should i do??? [Re: Slimz]
    #11221128 - 10/10/09 04:27 PM (8 years, 13 days ago)

Quote:

Slimz said:
when you toss some of the big fruit in the LC you may also toss in a few spores so even if it does not contam, its not isolated..




I dont get this at all...


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InvisibleShea25
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Re: What should i do??? [Re: CJCollin37]
    #11221207 - 10/10/09 04:43 PM (8 years, 13 days ago)

You can toss in a tissue sample from a mushroom into an LC to clone it but if you add spores its defeating the whole thing your back to Multispore. If you dont mind Mulitspore then just add spores into a LC it is easier and less likily to contams, but if you want a clone then toss in a tissue sample from what ever mushroom you like into the LC. I like using a sample from the inner stem also it helps to clone before they drop spores so theres no risk of getting spores into your Cloned LC


Edited by Shea25 (10/10/09 04:45 PM)


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Offlinefundamentalchair
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Re: What should i do??? [Re: Shea25]
    #11221609 - 10/10/09 05:58 PM (8 years, 13 days ago)




It seems like a clone would be an isolate...?




I suppose a short statement on how fungi grow would be in order.

A fungus isn't necessarily a singular organism, in nature it almost never is. Keep in mind that we're talking about genetic differences between a singular species, where species are allowed to interbreed. Think of it like dogs, a German Sheppard and a Labrador can breed. Does cloning their offspring create a pure-bread?

Fungi don't have a defined limit to their space as most animals do, but it's the same concept. You have multiple 'breeds' of fungus from a spore print and usually the strongest survive, or rather the strongers survive. In a perfect world, a fruit body would be an isolate, or close to one. Much like the game 'the weakest link', often an overpowering force of mediocrity will overpower the strong. The idea of isolation is to seperate those 'breeds' to find which are the strongest and use those. Due to genetic mutation, spores from an isolated strain will be multi-spore (although it will most likely create less isolates than a off-the-shelf multispore).

I'm not saying that cloning isn't an effective way to create a stronger grow, but it isn't isolation and it isn't as effective either.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light


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