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OfflineSalviaNshrooms9090
Farmer of the Herb
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Registered: 09/19/09
Posts: 113
Last seen: 8 years, 14 days
my 2nd grow???
    #11211431 - 10/09/09 12:01 AM (8 years, 17 days ago)

k first time i bought B+ from sporeyworks just like my 2nd time... 1st time i saw nothing no contams or mycelium i waited 25days... non viable.. got a new one.. inoculated 2 days ago no signs so far owell.. i inoculated 7 jars with 1ML a jar... the i used about 1.5 of of syringe to create a LC today... the 1.5 ML however was completely clear.. when i got syringe however it had a nice chunk of spores.. i shook it well after every jar./... i used this tek for the LC

http://www.shroomery.org/forums/showflat.php/Number/5239856#5239856

for the jars.. i used 2 cups  hoffman verm 1 cup bobs red mill BRF and 1 cup of refrigerator filtered water... sterilzed everything... so are my methods good?? if i dont see anything in the next week on both LC and Jars its safe to say spore syringe is non viable again??/

also.. how long will it take to see signs of mycelium in LC?? i have about 200ML of water and 1.5 ML of syringe...

if you give me a good answer ill rate you good..

good looks


Edited by SalviaNshrooms9090 (10/09/09 12:03 AM)


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Offlinenastos
secret secretions
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Registered: 06/15/09
Posts: 7,366
Last seen: 1 year, 2 months
Re: my 2nd grow??? [Re: SalviaNshrooms9090]
    #11211454 - 10/09/09 12:05 AM (8 years, 17 days ago)

it can take up to 15 days to see growth from MS. relax and be patient.. your method sounds good.. and just because you cant see spores doesnt mean they arent there.

I highly doubt spore works would send u 2 non viable syringes. most of the time is is the fault of the syringe holder and not storing it propely or not waiting long enough to let your jars cool or something

your lc should be pretty chunky in 6-10 days if u did everything right


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:shrug:


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OfflineNuzzle
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Registered: 09/29/08
Posts: 840
Last seen: 7 years, 10 months
Re: my 2nd grow??? [Re: nastos]
    #11213754 - 10/09/09 10:02 AM (8 years, 16 days ago)

More details would be needed on your sterilization methods.. Are you knocking up the jars in open air? Are you doing them after the jars have cooled down?
Are you flaming your syringe before each jar?

The more info you give, the more we can assist in whatever errors you might be making.
What size jars also?

I Can give you 3 pieces of advice...
1- Be patient
2- Be sterile as hell
3- Be very patient!

Good Luck!


--------------------
:hi:**If you have any spare spore prints lying around, and your feeling 'giving' drop me a PM** :dancer:


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OfflineSalviaNshrooms9090
Farmer of the Herb
Male

Registered: 09/19/09
Posts: 113
Last seen: 8 years, 14 days
Re: my 2nd grow??? [Re: Nuzzle]
    #11213918 - 10/09/09 10:41 AM (8 years, 16 days ago)

Quote:

Nuzzle said:
More details would be needed on your sterilization methods.. Are you knocking up the jars in open air? Are you doing them after the jars have cooled down?
Are you flaming your syringe before each jar?

The more info you give, the more we can assist in whatever errors you might be making.
What size jars also?

I Can give you 3 pieces of advice...
1- Be patient
2- Be sterile as hell
3- Be very patient!

Good Luck!





yes i sterilzed everything and let the jars cool for over 12 hours this time... i sprayed the room down a few hours before inoculation and cleaned the tupperware box with clorax and let that air in these settle and thats where i inoculated the jars.. so im sure everything was sterilze i sterilzed jars in pot for 1 hour 30 mins..didnt take jars out of the pot afterwards until i was ready to inoculate.. i flamed the needle on syringe until it was red hot and let it cool down all done in the sterile box... i put 1/4ML a hole for my 1 pint jars.. i have a dry layer of verm so i have the holes open and exposed on a shelf in the cleanest room in the house... its clean cuz no one is in it or anything.. the temp is around 76 - 80 ...


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OfflineSalviaNshrooms9090
Farmer of the Herb
Male

Registered: 09/19/09
Posts: 113
Last seen: 8 years, 14 days
Re: my 2nd grow??? [Re: SalviaNshrooms9090]
    #11216263 - 10/09/09 05:45 PM (8 years, 16 days ago)

bump... i love shrooms they are my favorite life would suck without


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