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Invisiblesounderror
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My jar Rapped My Syringe... * 1
    #11209692 - 10/08/09 07:57 PM (8 years, 16 days ago)

So got my syringe today, Did the Self-healing lid tek without a filter just one little sealed hole (Where I Screw Up), Anyways filled it with WBS after Following all the steps,
pressure cooked for an hour and a half
sterilize the shit outa my bathroom,
light up the alcohol lamp get all prepared,
swabbed the top of the jar with alcohol
waited a little for it to dry....
then stuck my needle in the hole.....


AND BAM!!!! the jar was like a vacuum and there goes 8.5Mls of my spores.. in about a second, shouldn't of been so nieeve
So i guess all i can do is hope for the best, and do a G2G transfer..


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Invisibleslackophage
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209711 - 10/08/09 08:00 PM (8 years, 16 days ago)

This a LC? Don't you want another breather hole w/polyfill when doing the self-healing thing?


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OfflineOthyem
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209726 - 10/08/09 08:02 PM (8 years, 16 days ago)

Yeah that happens when there isn't another hole for  air exchange. Also using grains you're going to need some type of filter for gas exchange.


Edited by Othyem (10/08/09 08:03 PM)


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InvisibleDoc_T
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209729 - 10/08/09 08:02 PM (8 years, 16 days ago)

So, uh... yeah.
You need to have a breather hole for WBS, it has to breathe. Gas Exchange, or GE.

You might be able to get some of that liquid back out of the jar if you can inject sterile air in until it equalizes or is slightly overpressure.


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InvisibleTacoHerder
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Re: My jar Rapped My Syringe... [Re: slackophage]
    #11209754 - 10/08/09 08:05 PM (8 years, 16 days ago)

IF you dont have afilter your jar is going to nothing but stall.  You will need to take a empty sterile syringe. Hold it over a open flame and suck in air, then insert the needle into the jar and let the jar suck all the air in.  Repeat that until the vacuum is gone.  But you still have no means for GE and your jar is still prob going to stall. is the lid metal or plastic?


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TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
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Invisiblesounderror
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Re: My jar Rapped My Syringe... [Re: Doc_T]
    #11209778 - 10/08/09 08:08 PM (8 years, 16 days ago)

yea the jar lid is metal, so if its stalled, then nothing will grow, so i can make new jars (when the tvek gets here), sterilize them then, use a glove box to do G2G transfer,


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InvisibleDoc_T
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Re: My jar Rapped My Syringe... [Re: TacoHerder]
    #11209783 - 10/08/09 08:08 PM (8 years, 16 days ago)

Dude could do an oldschool airport syringe. I don't have a link.


--------------------
You make it all possible. Doesn't it feel good?


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InvisibleTacoHerder
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209784 - 10/08/09 08:08 PM (8 years, 16 days ago)

you got any polyfill?


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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InvisibleDoc_T
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Re: My jar Rapped My Syringe... [Re: TacoHerder]
    #11209788 - 10/08/09 08:09 PM (8 years, 16 days ago)

Quote:

TacoHerder said:
you got any polyfill?




Polyfill, yep. Skip Tyvek for now.


--------------------
You make it all possible. Doesn't it feel good?


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Offlineshx764
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Re: My jar Rapped My Syringe... [Re: TacoHerder]
    #11209812 - 10/08/09 08:12 PM (8 years, 16 days ago)

Damn, thats a shame man. IMO your best bet would be to get a filter and a new lid ready with the added hole and swap them out in a glovebox.


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All labor that uplifts humanity has dignity and importance and should be undertaken with painstaking excellence.  - Martin Luther King, Jr.


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OfflineBDiddy
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Re: My jar Rapped My Syringe... [Re: shx764]
    #11209831 - 10/08/09 08:15 PM (8 years, 16 days ago)

Could he take a screwdriver flame it, then make a hole in he lid and stuff polyfill in it? Maybe?


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InvisibleDoc_T
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Re: My jar Rapped My Syringe... [Re: BDiddy]
    #11209845 - 10/08/09 08:18 PM (8 years, 16 days ago)

Probably not successfully.

This is just another life lesson, and a fairly cheap one. The lesson: read the tek. The cost: a spore syringe.


--------------------
You make it all possible. Doesn't it feel good?


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Invisiblesounderror
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209851 - 10/08/09 08:20 PM (8 years, 16 days ago)

Ok So Make A new Lid With PolyFill ASAP, but  dont exactly have any poly-fill, do you think i could just jack some from a stuffed animal?


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Invisiblefeelfunny
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Re: My jar Rapped My Syringe... [Re: Doc_T]
    #11209853 - 10/08/09 08:20 PM (8 years, 16 days ago)

how do people keep doing this?? is it not in the damn teks to presuize? i know there was in mine.  sorry to hear you live and learn i guess


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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Invisiblefeelfunny
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209857 - 10/08/09 08:21 PM (8 years, 16 days ago)

Quote:

sounderror said:
Ok So Make A new Lid With PolyFill ASAP, but  dont exactly have any poly-fill, do you think i could just jack some from a stuffed animal?





you can read the tag see what is in it. sometimes they will have 50% polly and 50% cotton


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Invisiblenoobieshroomie
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209858 - 10/08/09 08:21 PM (8 years, 16 days ago)

that would work too
just do it fast and in a GB

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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Offlinesdlocalkid85
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Re: My jar Rapped My Syringe... [Re: shx764]
    #11209862 - 10/08/09 08:22 PM (8 years, 16 days ago)

one thing you can do is wait for you jar to colonize then put it in your glovebox with a hammer and small nail and some micropore tape.  i know this isnt the best idea but it does work.  so is what your gonna want to do is get a good number of STERILE syringes and fill them with water, then PC them so they are sterile for sure. just let them cool to room temp and put them in your glovebox along with the hammer, nail, and tape.  now sterilize the shit out of your gb.  now flame the tip of the small nail and puncture a hole in the lid, immediatley cover it with micropore tape.  now use you syringes to inject a bunch of water into the jar.  now you will have mycelium water floating around in there.  so now you want to suck the mycelium water up in your sterile syringes and now you got an LC.  i wouldn't suggest this but i have read about people doing it.  if it was your only syringe it is kinda your only choice unless you get another.  you can use the mycelia water to inoculate a LC or use it to knock up some jars.  good luck man


--------------------
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Invisiblesounderror
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Re: My jar Rapped My Syringe... [Re: Doc_T]
    #11209865 - 10/08/09 08:22 PM (8 years, 16 days ago)

Quote:

Doc_T said:
Probably not successfully.

This is just another life lesson, and a fairly cheap one. The lesson: read the tek. The cost: a spore syringe.



haha yea i know :frown: i did read it, but i was in patient which is not good especially for growing shrooms, but lesson learned, it was kinda an expensive mess up, i dont have a credit card or anything so i have to go get one of those prepaid visa's which was like $30 bucks but could be worse,


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Invisiblefeelfunny
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Re: My jar Rapped My Syringe... [Re: sounderror]
    #11209876 - 10/08/09 08:23 PM (8 years, 16 days ago)

i can send you a print  if you want just PM but you have to grow and show when you get


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Invisiblesounderror
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Re: My jar Rapped My Syringe... [Re: feelfunny]
    #11209879 - 10/08/09 08:24 PM (8 years, 16 days ago)

Quote:

feelfunny said:
Quote:

sounderror said:
Ok So Make A new Lid With PolyFill ASAP, but  dont exactly have any poly-fill, do you think i could just jack some from a stuffed animal?





you can read the tag see what is in it. sometimes they will have 50% polly and 50% cotton



Alright well im gonna go raid the stuffed animals,


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