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InvisibleImorph
journeyman
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Registered: 11/10/01
Posts: 146
Fish Juice Anti-Senescence Elixir ?
    #11208448 - 10/08/09 04:43 PM (8 years, 2 months ago)

hello all in reading the post about using Fish Emulsion i remembered a old post proposing a

method of revitalizing  old and  unvigorous cultures (Senescence ) by adding an a bit of insect (mash?)  to agar then pc. maybe this tek would be applicable to fish emulsion. 

The post on fish emulsion got me to thinking a little. Since it's not the normal food of edible fungi perhaps it could re-invigorized a culture in the dreaded Senescence State?  i just happened have to have some Fish emul in storage....the link to the original post is below. Anyone care to pipe in go ahead. i posted the jist  and link of story below.

http://www.shroomery.org/forums/showflat.php/Number/7749701#7749701

poster Jemery Davis

"John Holiday of Aloha Medicinals:
He suggested using a cricket agar made from putting crickets he bought at the pet store into a

blender. His reasoning is that when a culture gets used to a media/substrate it becomes lazy

because it is used to creating the same enzymatic pathways for the breakdown of organic matter

into nutrition. By giving it something altogether different and new, you are challenging it,

helping it exercise those hyphae!

He says it grows very slowly on the cricket agar (or whatever you've used), but when you transfer

it back onto a more traditional agar media, you really see the growth take off. So that

represents one way you could go and get yourself a nice commercial culture... "

i am going to be trying some oyster cultures sometime soon so maybe a few drops add to a sterile plate then innoc?


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OfflinebioBot

Registered: 05/02/09
Posts: 275
Last seen: 3 months, 26 days
Re: Fish Juice Anti-Senescence Elixir ? [Re: Imorph]
    #11216280 - 10/09/09 05:48 PM (8 years, 2 months ago)

The concept makes sense, but I still have to
question whether long term results can be achieved.

As I've always seen it variations in substrate
composition are more of a benefit to the health of
a particular mycelium as it relates to fruiting
performance.

Senescence is a genetic issue that is different
from one strain to the next...  Its based on DNA
fragmentation or something - I'm not fully versed
in all the details surrounding it.


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InvisibleImorph
journeyman
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Registered: 11/10/01
Posts: 146
Re: Fish Juice Anti-Senescence Elixir ? [Re: bioBot]
    #11222734 - 10/10/09 09:21 PM (8 years, 2 months ago)

hello bioBot,

thank you for the input. the subject of senescence was just something that i thought of after reading a

bit of the post on Fish Emulsion. it was just a idea that came to me that perhaps this senescence thing

could be turned around since i had remembered reading about the addition  of a insect material to agar as

a way to reawakened the vigor of mycelium for the usual plant substrates.

i will concur with you tho that ultimately senescence is probably errors in cell re genesis systems...ie

human aging, cancer and mutations. So we should look in awe at some tortoises,the Majestic Sequoias and

the gnarly Brislecone Pines. apparently they  have the ability to maintain loyal faithful cellular generation

in spite of cosmic rays or environmental adversities over a long period. Still

every thing that is not the Absolute or Divine is subject to the
"crossover" in time. but still my fellow

fungus devotee maybe in some kinds of senescence there's something
( reversal? ) to what Jeremy Davis

claimed to have observed. Of course the only way is to try and

replicate what he reported to have noted. so this would me some sample number (?) then a split between

control and experimental group with all other things being equal. Then looking at growth rates of a single

non vigorous strain then with of other diff low vigor strains....of course we

have to eliminate  possible substrate problems, " cryptic and hidden contams " as chief adversaries. We

then make some inference from findings. Of course i am not so energetic to do this in a large and proper

way so i ll just add  a crushed bug or drops of fish juice  to a couple of plates when i pc/autoclave a big load. you know a few

small trials from time to time ?

besides i am having enough trouble growing successful common and not so (V. volvacea) common strains. keep

me posted and get rid of all contams. imorph


Edited by Imorph (10/11/09 07:48 PM)


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