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Mushrooms, Mycology and Psychedelics > Mushroom Hunting and Identification
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Inocybe species with bluing
    #11208086 -

Newly Determined to find Psilocybe pelliculosa I grabbed my packing gear, my camera, and a bottle of water and hit the road. On the outskirts of Thurston County, WA, is an area that is frequented by campers and hikers.

It was around 10am and it was very cold today, probably one of the coldest days so far this Fall. Instead of being "judicial" and saying, "no" "nope" and "no way," I figured I had room to learn and it couldn't harm me to pick up a few extra mushrooms and observe them. While picking a very thin and rather unimportant looking mushroom as I was knelt down, my eyes stumbled upon this:

Fuck I'm out of quota for images. I just renewed and I'm waiting for it to get updated before I can upload these.

Edited by World Spirit (12/29/09 05:46 PM)

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Re: Today's Discovery! [Re: World Spirit]
    #11208120 -

Hmm, how did you renew, I thought the only way to get more upload space was to become a supporter or delete images?
inski.


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Re: Today's Discovery! [Re: inski]
    #11208128 -

I had to add it to my shopping cart (supporter account). Expired 24hrs ago.

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Re: Today's Discovery! [Re: World Spirit]
    #11208165 -

Oh, I see, what method do you use to pay for the supporter accounts?
inski.


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Re: Today's Discovery! [Re: inski]
    #11208175 -

Ummm let me PM you...

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Re: Today's Discovery! [Re: World Spirit]
    #11208263 -

why not upload it to tinypic / imageshack?


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actually my posts suck but i have a funny sig


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Re: Today's Discovery! [Re: batheinthefountain]
    #11208377 -

I honestly cringe whenever I have to go to those sites to view images. I feel like I've dirtied myself for some funny reason. I'm close to just emailing the images to one of you guys.

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Re: Today's Discovery! [Re: inski]
    #11208443 -

























[url=https://files.shroomery.org/files/09-41/503430699-14.jpg]

[/url]

















Growing underneath a tree (unidentified as I'm not good at that yet) and growing in the middle of the woods on the edge of a trail. Substrate was mainly soil and what I think are pine needles or some kind of shorter, darker needle (you tell me guys). I had sticky stuff on my hands but supposedly pines don't grow in that area.

Aroma: It took me about twenty or thirty sniffs before I could determine what it was I was smelling. It was VERY pleasant and actually made me think of eating something very nice. Around the last smell I finally said, It smells like something I've bought at a nicer bakery.

Bruising: Upon picking immediate but difficult to observe bluing occurred closer to the base of the stem about an half an inch to an inch up. With more handling the bluing was more definitive but still lacking a class Cube type of bruising.

Average height was about 6 centimeters or 2.25 inches.

Average cap width was over 1/2 inch.

Additional Notes: The stem also bruised a rust color on a finer set of thick hair-like attachments on the surface of the stipe. At the very base there was a dark rust stain. The same was true of the cap; there were areas of rust on it.

When cut in half, the stem is not hollow and after about ten minutes the area turns to a rusty color.

The cap gills are white but I don't think the spore print will come out white. The gills are close together.

With the exception of that one cluster they were growing somewhat scattered and individually yet not too far off from each other. Collected about ten specimens. Stardate Oct 8th, 2009, in WA.

World Spirit

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Re: Today's Discovery! [Re: World Spirit]
    #11208461 -

Looks like Inocybe calamistrata, nice finds!
inski.


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Re: Today's Discovery! [Re: inski]
    #11208512 -

Thanks for the id, my friend. I was not even aware of that species existing. In case anyone else is new to it...
http://mushroomobserver.org/image/show_image/11088?obs=6689&search_seq=843403&seq_key=724551

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Re: Today's Discovery! [Re: inski]
    #11208529 -

inski said:
Looks like Inocybe calamistrata, nice finds!
inski.



Beat me to it.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

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Re: Today's Discovery! [Re: Workman]
    #11208569 -

Hi Workman, I'd like to see a microscopy composite of these mushrooms, I always find Inocybe species to have interesting microscopic features!
inski.


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Re: Today's Discovery! [Re: inski]
    #11208597 -

I've just read Stamets' writeup of Inocybe and have to wonder if everyone in the mycological side of the house has concluded these are too dangerous to bioassay(?)

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Re: Today's Discovery! [Re: World Spirit]
    #11208627 -

I agree, I would advise against consuming any Inocybe species, I would preserve some specimens for study though!
Nice images by the way:cool:
inski.


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Re: Today's Discovery! [Re: inski]
    #11209068 -

I can probably do a composite.  That species grows in my area so I'll look around.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

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Re: Today's Discovery! [Re: World Spirit]
    #11209188 -

Quote:
I honestly cringe whenever I have to go to those sites to view images.


The proper way to do it is to post the observation on mushroomobserver.org, then copy the image location, and paste the image URL between [img] tags.

The /640/ size is about right but you can also include thumbnail size or full size images.

I rarely upload images to the shroomery, almost all of my posts are from external sites.  By [img] including the images, no one has to click on each image to view it, which is the part that sucks.

Using mushroomobserver for hosting gives the additional benefit of having more eyes looking at it. 

I use photobucket for the images which don't always have mushrooms, like these. 

I agree that this is close to I. calamistrata.  There are several species in that group that look similar, with different odors and amounts of stem and cap shagginess.

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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11733522 -

Not to drudge up an old post but this collection is still around for KOH testing and I now need to ask for the best method to go about it. I have KOH, 500x microscope, camera, slides, slide covers, bottled water, and the specimens. I'm not sure if I'm supposed to mix a "dash" of KOH with a teaspoon of water, etc.

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Re: Today's Discovery! [Re: World Spirit]
    #11733618 -

I don't think you can use KOH as an indicator of active chemicals, it's just used for rehydrating and making mounts of material for viewing through the microscope!
inski.


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Re: Today's Discovery! [Re: World Spirit]
    #11733625 -

Use 5% weight / weight aqueous solution.

Please post some micrographs.

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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11733675 -

Alright I'm clearly going to have to eyeball the measurement which I'm good at but the micrograph thing with my unsteady hand is going to be difficult.

Here's what I understand to do:
1. Take the most strongly bluing area of a specimen and chop it into very fine pieces
2. Take a pinch of those fine pieces and place on a microscope slide
3. Add a few drops of the 95/5 water/koh solution and await a color reaction
4. Take photos at 500x under microscope and await further input

World Spirit is as symbiotic as any mushroom that has this built into their DNA. He awaits on the relations of his friends to be successful in both minor and major activities in Life.

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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11733686 -

Going by World Spirits post here I'm assuming that he's asking wether he can test for psilocybin using this chemical, it's just used for rehydrating and mounting dried material and checking for certain reactions in other genera, not the presence of active chemicals but please do make some micrographs, Inocybe species have quite interesting micro characters!
inski.


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Re: Today's Discovery! [Re: inski]
    #11733792 -

I also have p-dmab. It's been so long since we vaguely discussed this that I can't remember which one to use to see if active components are present.

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Re: Today's Discovery! [Re: World Spirit]
    #11733814 -

That's the one you need to use but I'm not sure what method you would use, Alan can tell you!
inski.


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Re: Today's Discovery! [Re: inski]
    #11733928 -

Quote:
Here's what I understand to do:
1. Take the most strongly bluing area of a specimen and chop it into very fine pieces
2. Take a pinch of those fine pieces and place on a microscope slide
3. Add a few drops of the 95/5 water/koh solution and await a color reaction
4. Take photos at 500x under microscope and await further input


What do you expect that to do?

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Re: Today's Discovery! [Re: inski]
    #11733929 -

:shineon:  The we wait!  :captain:

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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11733940 -

I guess I mixed up what does what for testing for the presence of psilocybin. I have p-dmab and my memory is telling me I'm looking for a color change, but to be quite frank, I have not read any clear instructions as to how to proceed and what to look for.

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Re: Today's Discovery! [Re: World Spirit]
    #11733973 -

Did you get my PM, World Spirit?

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Re: Today's Discovery! [Re: elprawn]
    #11733996 -

:thumbup:

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Re: Today's Discovery! [Re: World Spirit]
    #11734203 -

Based on what I just read here ---->  http://www.shroomery.org/forums/showflat.php/Number/10769530
I think it's safe to say that anyone without access to a college laboratory with an experienced person using a GC/MS device isn't going to get to a beneficial point of knowledge.

I'm going to contact the colleges in my area tomorrow and see if I can get to the bottom of this species. If you are a chemistry student in Olympia and have access to the staff and lab I'd love to hear from you.

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Re: Today's Discovery! [Re: World Spirit]
    #11735015 -

I found some mushrooms that were ID'd as Inocybe calamistrata earlier this year and they were a much darker brown.










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Re: Today's Discovery! [Re: CubeBensies]
    #11736061 -

Thanks for posting those images. This is another aspect worth questioning. I've seen both types pictured and identified as Inocybe calamistrata and wondered if there are two or more varieties or if insufficient identification took place.

Does anyone else have collections with decent photographs with bluing?

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Re: Today's Discovery! [Re: World Spirit]
    #11736435 -

I seem to recall that there are some recognized varieties.  I'll see if I can dig up the reference.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

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Re: Today's Discovery! [Re: Workman]
    #11736495 -

Workman said:
I seem to recall that there are some recognized varieties.  I'll see if I can dig up the reference.


Please do!


--------------------

:sun:Variety is the spice of life!:sun:



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Re: Today's Discovery! [Re: World Spirit]
    #11736770 -

Quote:
Based on what I just read here ---->  http://www.shroomery.org/forums/showflat.php/Number/10769530
I think it's safe to say that anyone without access to a college laboratory with an experienced person using a GC/MS device isn't going to get to a beneficial point of knowledge.




GC/MS / lab equipment is not required to test for psilocybin. 

http://www.shroomery.org/forums/showflat.php/Number/9051584#9051584

http://www.shroomery.org/forums/showflat.php/Number/9048128


Quote:
I've seen both types pictured and identified as Inocybe calamistrata and wondered if there are two or more varieties or if insufficient identification took place.



There are a few species close to I. calamistrata that look similar.  I. calamistratoides is one.

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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11736872 -

Quote:
Alan Rockefeller said:
There are a few species close to I. calamistrata that look similar.  I. calamistratoides is one.


Those look fantastic. :bliss:


--------------------

:sun:Variety is the spice of life!:sun:



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Re: Today's Discovery! [Re: Alan Rockefeller]
    #11736938 -

i can get some samples for anyone who may be interested come next september.ive been seeing these bluing inocybes a few years in a row at one of my hunting areas always around sept.some have been pretty big with intense bluing/greening running more than halfway up the stems.

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Re: Today's Discovery! [Re: greatfulhunters]
    #11738670 -

Here's an article showing not only two "varieties" but also two "forms," a piece of nomenclature new to me:  http://data.gbif.org/species/browse/taxon/8739399

Variety: Inocybe calamistrata var. calamistrata
Variety: Inocybe calamistrata var. mucidiolens
Form: Inocybe calamistrata f. calamistrata
Form: Inocybe calamistrata f. gracilis



Another important note is in the book Mushrooms of the Pacific Northwest. There is no official entry for Inocybe calamistrata but it's noted within Inocybe hirsuta var maxima. I quote:
"Often identified as Inocybe calamistrata, Inocybe hirsuta var. maxima is a medium to large rubust (for an Inocybe), shaggy-scaly mushroom with reddish brown coloration; the flesh, stipe and gills bruise reddish, and the base of the stipe is usually greenish blue."

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Re: Today's Discovery! [Re: World Spirit]
    #11744944 -
Log in to view attachment

Attached are specifically pages 46-50 of German Mycologist Jochen Gartz's Magic Mushrooms Around the World (no longer in print and now an artifact). It discusses the chemical analysis of multiple Inocybe species testing positive for psilocybin.

I imagine Gartz's work is not without scrutiny at the present moment.


I have ignorantly begun a koh/water 5/95 liquid drop test comparing the reactions of Psilocybe cyanescens and the Inocybe spp. collection above. After several minutes no reaction worth noting has occurred.

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Re: Today's Discovery! [Re: World Spirit]
    #11760970 -




I'm holding off on trying my own hand and instead I've contacted Evergreen S.C. to see if they will allow me to bring in the collection for GC/MS testing in their science/chemistry department.

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Re: Today's Discovery! [Re: World Spirit]
    #11761001 -

i have seem more bluing in the inocybe i found here .
aparently some one eat some inocybe  posible calamistrata  some weeks ago and experience the muscarine effects.


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo

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Re: Today's Discovery! [Re: cactu]
    #11761037 -

Can you get a detailed description of their experience, including "side effects" and potentially positive aspects of their experience?

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Re: Today's Discovery! [Re: World Spirit]
    #11761081 -

well alan  told me about it here it is
http://www.shroomery.org/forums/showflat.php/Number/11600795#11600795


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo

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Re: Today's Discovery! [Re: cactu]
    #11761253 -

That's quite an interesting read and I only looked at 1/5th of those posts.

Unless there was a conclusion with certainty that those are Inocybes I'm still left with a curiosity to see exactly what molecules will be found in GC/MS - for better or worse. It presents a religious question if it contains psilocybin, in my mind.

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Mushrooms, Mycology and Psychedelics > Mushroom Hunting and Identification
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