|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Can sclerotia be used to colonize grain directly?
#11192996 - 10/06/09 11:14 AM (14 years, 5 months ago) |
|
|
Anyone tried this? I'm just curious.
I did a little searching, and I found other people had asked this question but I could not find any direct and unambiguous answer.
Put as plainly as possible: is dropping one or more harvested & cleaned sclerotia into a jar of fresh grain a viable method for colonizing the jar?
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
#11193023 - 10/06/09 11:20 AM (14 years, 5 months ago) |
|
|
About as viable as doing it with a piece of mushroom tissue, is my guess. Possible but unlikely- use agar for a more certain outcome.
-------------------- You make it all possible. Doesn't it feel good?
|
apoonanor
Time & Space Traveler
Registered: 04/20/09
Posts: 380
Last seen: 9 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
#11193045 - 10/06/09 11:24 AM (14 years, 5 months ago) |
|
|
sclerotia is essentially balls of myc. using sclerotia to inoculate grain would be the same as doing a grain to gran transfer unless they sclerotia has already been dried out. then, the chances decrease exponentially the older it is. hope this helps.
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: apoonanor]
#11193068 - 10/06/09 11:28 AM (14 years, 5 months ago) |
|
|
Hmm, some disagreement.
Gonna see if I can't reel in some more replies.
|
HybridprX
Biodegrader of coir
Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 6 years, 6 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
#11193074 - 10/06/09 11:29 AM (14 years, 5 months ago) |
|
|
scleortia decompose just like every other fungi, once in the decomposition process it breaks back down into mycelium and will regrow, cutt open the scleortia with a exacto knife or scalpel and take a cutt from the inner tissue and drop it into the grain jar in front of a glovebox or flowhood.
Only take tissue clones from prolific fruiting it's not worth the time to clone poor preforming genetics unless you feel like experimenting for when the time comes.
--------------------
|
feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
|
Re: Can sclerotia be used to colonize grain directly? [Re: HybridprX]
#11193130 - 10/06/09 11:40 AM (14 years, 5 months ago) |
|
|
thats what i do
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Can sclerotia be used to colonize grain directly? [Re: feelfunny]
#11193175 - 10/06/09 11:47 AM (14 years, 5 months ago) |
|
|
Has anyone tried it with stones into grain? Or is it theoretical?
-------------------- You make it all possible. Doesn't it feel good?
|
HybridprX
Biodegrader of coir
Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 6 years, 6 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
#11193210 - 10/06/09 11:52 AM (14 years, 5 months ago) |
|
|
I've never done it using sclerotia but it's a fruit body from a fungi, same deal as everything else.
--------------------
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: HybridprX]
#11193265 - 10/06/09 12:03 PM (14 years, 5 months ago) |
|
|
Sclerotia aren't really "fruiting bodies" -- as far as I understand it, they have no reproductive purpose, but are essentially a little "myc fortress" to keep some of the colony alive through harsh weather conditions.
I would presume that this means they'd be well-suited to the purpose of colonizing grain ... but if nobody has tried it ...
I'm gonna remain skeptical with Doc_T. Dude's never led me wrong in the past.
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
#11193305 - 10/06/09 12:11 PM (14 years, 5 months ago) |
|
|
"Cautiously optimistic", I guess. I don't know why it wouldn't work- but somebody needs to do it and post results.
-------------------- You make it all possible. Doesn't it feel good?
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
#11193311 - 10/06/09 12:12 PM (14 years, 5 months ago) |
|
|
If nobody's done it in a month or two, I'll have the opportunity to test it out then.
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
#11193319 - 10/06/09 12:13 PM (14 years, 5 months ago) |
|
|
Sclerotia are completely capable of colonizing a substrate, thats kinda the point of them, they maintain a microcosm of life and wait for conditions to get better.. i would think that being surrounded by moist grain would constitute a better condition..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
#11193330 - 10/06/09 12:15 PM (14 years, 5 months ago) |
|
|
Yeah exactly; it seems like they should be perfect, 'cause (from what I understand) that's what they're "made" for. But until someone tries it, that's just supposition.
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
#11193381 - 10/06/09 12:23 PM (14 years, 5 months ago) |
|
|
i would say throw the stone in some peroxide and then do a sterile transfer unless the stone is still in a sterile environment.
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
|
Re: Can sclerotia be used to colonize grain directly? [Re: Slimz] 1
#11193387 - 10/06/09 12:24 PM (14 years, 5 months ago) |
|
|
or just cut it open that is what i do
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
#11193388 - 10/06/09 12:24 PM (14 years, 5 months ago) |
|
|
That's basically what I was thinking. BTW, can you shed any light on what the peroxide is for? I have only a vague idea.
|
laserpig
Weedmaster_P
Registered: 04/28/09
Posts: 7,468
Last seen: 11 years, 10 months
|
Re: Can sclerotia be used to colonize grain directly? [Re: feelfunny]
#11193394 - 10/06/09 12:25 PM (14 years, 5 months ago) |
|
|
Wait so you're saying that you colonize grain with sclerotia?
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
#11193409 - 10/06/09 12:28 PM (14 years, 5 months ago) |
|
|
peroxode would just kill any spores that were hanging around the stone (like trich etc)
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
#11193435 - 10/06/09 12:32 PM (14 years, 5 months ago) |
|
|
Peroxide doesn't kill spores. But it would help wash them away. Are you better off with a whole stone or a tissue section?
-------------------- You make it all possible. Doesn't it feel good?
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
#11193471 - 10/06/09 12:37 PM (14 years, 5 months ago) |
|
|
nature would say whole stone..
feelfunny says cut it open
i say cut into quarters as to allow alot of internal surface area to touch the grain..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
|