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Offlinelaserpig
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Can sclerotia be used to colonize grain directly?
    #11192996 - 10/06/09 01:14 PM (8 years, 1 month ago)

Anyone tried this? I'm just curious.

I did a little searching, and I found other people had asked this question but I could not find any direct and unambiguous answer.

Put as plainly as possible: is dropping one or more harvested & cleaned sclerotia into a jar of fresh grain a viable method for colonizing the jar?


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InvisibleDoc_T
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Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
    #11193023 - 10/06/09 01:20 PM (8 years, 1 month ago)

About as viable as doing it with a piece of mushroom tissue, is my guess. Possible but unlikely- use agar for a more certain outcome.


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Offlineapoonanor
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Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
    #11193045 - 10/06/09 01:24 PM (8 years, 1 month ago)

sclerotia is essentially balls of myc. using sclerotia to inoculate grain would be the same as doing a grain to gran transfer unless they sclerotia has already been dried out. then, the chances decrease exponentially the older it is. hope this helps.


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: apoonanor]
    #11193068 - 10/06/09 01:28 PM (8 years, 1 month ago)

Hmm, some disagreement. :strokebeard:

Gonna see if I can't reel in some more replies.  :fishing:


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OfflineHybridprX
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Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
    #11193074 - 10/06/09 01:29 PM (8 years, 1 month ago)

scleortia decompose just like every other fungi, once in the decomposition process it breaks back down into mycelium and will regrow, cutt open the scleortia with a exacto knife or scalpel and take a cutt from the inner tissue and drop it into the grain jar in front of a glovebox or flowhood.

Only take tissue clones from prolific fruiting it's not worth the time to clone poor preforming genetics unless you feel like experimenting for when the time comes.


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Re: Can sclerotia be used to colonize grain directly? [Re: HybridprX]
    #11193130 - 10/06/09 01:40 PM (8 years, 1 month ago)

thats what i do


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Re: Can sclerotia be used to colonize grain directly? [Re: feelfunny]
    #11193175 - 10/06/09 01:47 PM (8 years, 1 month ago)

Has anyone tried it with stones into grain? Or is it theoretical?


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OfflineHybridprX
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Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
    #11193210 - 10/06/09 01:52 PM (8 years, 1 month ago)

I've never done it using sclerotia but it's a fruit body from a fungi, same deal as everything else.


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: HybridprX]
    #11193265 - 10/06/09 02:03 PM (8 years, 1 month ago)

Sclerotia aren't really "fruiting bodies" -- as far as I understand it, they have no reproductive purpose, but are essentially a little "myc fortress" to keep some of the colony alive through harsh weather conditions.

I would presume that this means they'd be well-suited to the purpose of colonizing grain ... but if nobody has tried it ...

I'm gonna remain skeptical with Doc_T. Dude's never led me wrong in the past.


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Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
    #11193305 - 10/06/09 02:11 PM (8 years, 1 month ago)

"Cautiously optimistic", I guess. I don't know why it wouldn't work- but somebody needs to do it and post results.


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
    #11193311 - 10/06/09 02:12 PM (8 years, 1 month ago)

If nobody's done it in a month or two, I'll have the opportunity to test it out then. :shrug:


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InvisibleSlimz
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Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
    #11193319 - 10/06/09 02:13 PM (8 years, 1 month ago)

Sclerotia  are completely capable of colonizing a substrate, thats kinda the point of them, they maintain a microcosm of life and wait for conditions to get better.. i would think that being surrounded by moist grain would constitute a better condition..


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
    #11193330 - 10/06/09 02:15 PM (8 years, 1 month ago)

Yeah exactly; it seems like they should be perfect, 'cause (from what I understand) that's what they're "made" for.
But until someone tries it, that's just supposition.


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InvisibleSlimz
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Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
    #11193381 - 10/06/09 02:23 PM (8 years, 1 month ago)

i would say throw the stone in some peroxide and then do a sterile transfer unless the stone is still in a sterile environment.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
    #11193387 - 10/06/09 02:24 PM (8 years, 1 month ago)

or just cut it open that is what i do


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
    #11193388 - 10/06/09 02:24 PM (8 years, 1 month ago)

That's basically what I was thinking.
BTW, can you shed any light on what the peroxide is for? I have only a vague idea.


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Offlinelaserpig
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Re: Can sclerotia be used to colonize grain directly? [Re: feelfunny]
    #11193394 - 10/06/09 02:25 PM (8 years, 1 month ago)

Wait so you're saying that you colonize grain with sclerotia?


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InvisibleSlimz
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Re: Can sclerotia be used to colonize grain directly? [Re: laserpig]
    #11193409 - 10/06/09 02:28 PM (8 years, 1 month ago)

peroxode would just kill any spores that were hanging around the stone (like trich etc)


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: Can sclerotia be used to colonize grain directly? [Re: Slimz]
    #11193435 - 10/06/09 02:32 PM (8 years, 1 month ago)

Peroxide doesn't kill spores. But it would help wash them away.
Are you better off with a whole stone or a tissue section?


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InvisibleSlimz
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Re: Can sclerotia be used to colonize grain directly? [Re: Doc_T]
    #11193471 - 10/06/09 02:37 PM (8 years, 1 month ago)

nature would say whole stone..

feelfunny says cut it open

i say cut into quarters as to allow alot of internal surface area to touch the grain..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
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