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OfflineSS32
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Registered: 07/27/09
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LC Colonized, still need GE?
    #11192235 - 10/06/09 10:21 AM (8 years, 2 months ago)

One of my LCs FINALLY worked and I have a good cloud of myc growing.  I'm happy with where it is and want to save it in the fridge.  I wasn't thinking and didn't put a hole for gas exchange in the lid, so all I have is the self healing port.  Right now, I have a syringe with a coffee filter in the port to allow for GE.  Once I put it in the fridge, will it still need fresh air?  Or am I safe just pulling the syringe and allowing the port to close?


--------------------
Did he dream about dragons? Did he dream about deer? Did he whisper  he names of friends who were near?
What songs did they play, and how far away? Why did he whisper, why did she scream?
What does the sound of a screen door mean?
Who talks on the hill? Who goes to the cellar, can you feel the chill? Where does the river, when will the wind?
How far are the mountains? Where do they end? Why would the church?
Did the service begin? Tell me who died, and tell me who cried.
Help me hide in the skin of a deer, my zippered-up bag in the mouth of a stag so swiftly I go through rows of does,
it flows, it flows, it flows, it flows all over the hill where the green grass grows.


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InvisibleDoc_T
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Re: LC Colonized, still need GE? [Re: SS32]
    #11192241 - 10/06/09 10:24 AM (8 years, 2 months ago)

It's ok with the air that's in it.
Did you shoot a test jar? Better do that before you get too excited.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineSS32
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Re: LC Colonized, still need GE? [Re: Doc_T]
    #11192276 - 10/06/09 10:34 AM (8 years, 2 months ago)

No, not yet.  But, I'm hopeful.  Yesterday, there were a couple balls of "fingers" that look just like the myc in my jars and today they've joined into a bigger mass.  I'm going to cook some jars this afternoon and test them.


--------------------
Did he dream about dragons? Did he dream about deer? Did he whisper  he names of friends who were near?
What songs did they play, and how far away? Why did he whisper, why did she scream?
What does the sound of a screen door mean?
Who talks on the hill? Who goes to the cellar, can you feel the chill? Where does the river, when will the wind?
How far are the mountains? Where do they end? Why would the church?
Did the service begin? Tell me who died, and tell me who cried.
Help me hide in the skin of a deer, my zippered-up bag in the mouth of a stag so swiftly I go through rows of does,
it flows, it flows, it flows, it flows all over the hill where the green grass grows.


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Invisibletruskool
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Re: LC Colonized, still need GE? [Re: SS32]
    #11192282 - 10/06/09 10:36 AM (8 years, 2 months ago)

LC's can look pristine and still have all sorts of nasties in it shoot a test jar or be willing to lose all the jars that you cook up.


--------------------
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Offlinecloudsaregathering
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Re: LC Colonized, still need GE? [Re: truskool]
    #11192303 - 10/06/09 10:42 AM (8 years, 2 months ago)

yeah test first, be safe later...


--------------------

"the root of the problem has been isolated"


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Offlinecloudsaregathering
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Re: LC Colonized, still need GE? [Re: cloudsaregathering]
    #11192306 - 10/06/09 10:43 AM (8 years, 2 months ago)

I just had to throw some out, looked like pieces of baby poop floating in the jar...


--------------------

"the root of the problem has been isolated"


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Offlinebeto
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Re: LC Colonized, still need GE? [Re: cloudsaregathering]
    #11192313 - 10/06/09 10:46 AM (8 years, 2 months ago)

You mean something like this:



That's my lc, i used to colonize a jar and some petri dishes. I can confirm its myc. Today i checked this jar again and myc cloud has grown twice it's size already. It really grows fast in liquid medium isn't?


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Invisibletruskool
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Re: LC Colonized, still need GE? [Re: beto]
    #11192330 - 10/06/09 10:49 AM (8 years, 2 months ago)

Yes LC's rock:headbang3:
Now that I was finally able to get some karo they are the best.  LC's take off like a rocket.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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OfflineSS32
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Re: LC Colonized, still need GE? [Re: truskool]
    #11193321 - 10/06/09 02:13 PM (8 years, 2 months ago)

Damnit, after looking at those pictures, I think I have a contam.  That makes 4 failed LCs using the beginner friendly tek.




--------------------
Did he dream about dragons? Did he dream about deer? Did he whisper  he names of friends who were near?
What songs did they play, and how far away? Why did he whisper, why did she scream?
What does the sound of a screen door mean?
Who talks on the hill? Who goes to the cellar, can you feel the chill? Where does the river, when will the wind?
How far are the mountains? Where do they end? Why would the church?
Did the service begin? Tell me who died, and tell me who cried.
Help me hide in the skin of a deer, my zippered-up bag in the mouth of a stag so swiftly I go through rows of does,
it flows, it flows, it flows, it flows all over the hill where the green grass grows.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
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Re: LC Colonized, still need GE? [Re: SS32]
    #11193333 - 10/06/09 02:16 PM (8 years, 2 months ago)

You can't tell anything by looking.
And is this the no-PC tek?


--------------------
You make it all possible. Doesn't it feel good?


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Invisiblefeelfunny
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Re: LC Colonized, still need GE? [Re: truskool]
    #11193344 - 10/06/09 02:18 PM (8 years, 2 months ago)

Quote:

truskool said:
Yes LC's rock:headbang3:
Now that I was finally able to get some karo they are the best.  LC's take off like a rocket.





glad you finally got it down


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Invisiblebotanisthype
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Re: LC Colonized, still need GE? [Re: SS32]
    #11193361 - 10/06/09 02:20 PM (8 years, 2 months ago)

heres some healthy pics..

KC-



MAZ-



--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol


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Invisiblefeelfunny
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Re: LC Colonized, still need GE? [Re: botanisthype]
    #11193370 - 10/06/09 02:22 PM (8 years, 2 months ago)



--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Offlinebeto
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Re: LC Colonized, still need GE? [Re: SS32]
    #11193416 - 10/06/09 02:29 PM (8 years, 2 months ago)

Quote:

SS32 said:
Damnit, after looking at those pictures, I think I have a contam.  That makes 4 failed LCs using the beginner friendly tek.








Like one said, u cant tell if it's contaminated, only testing it. My cloud looks like rizomorphic myc, and its healthy cause i noc up a jar and some dishes.


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OfflineSS32
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Re: LC Colonized, still need GE? [Re: Doc_T]
    #11193485 - 10/06/09 02:39 PM (8 years, 2 months ago)

Quote:

Doc_T said:
You can't tell anything by looking.
And is this the no-PC tek?




Correct.  I'll knock up a couple jars to make sure.


--------------------
Did he dream about dragons? Did he dream about deer? Did he whisper  he names of friends who were near?
What songs did they play, and how far away? Why did he whisper, why did she scream?
What does the sound of a screen door mean?
Who talks on the hill? Who goes to the cellar, can you feel the chill? Where does the river, when will the wind?
How far are the mountains? Where do they end? Why would the church?
Did the service begin? Tell me who died, and tell me who cried.
Help me hide in the skin of a deer, my zippered-up bag in the mouth of a stag so swiftly I go through rows of does,
it flows, it flows, it flows, it flows all over the hill where the green grass grows.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblenoobieshroomie
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Re: LC Colonized, still need GE? [Re: SS32]
    #11193541 - 10/06/09 02:47 PM (8 years, 2 months ago)

more healthy LC


a chunk of agar was put in this one


heres the plate it came from


  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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InvisibleDoc_T
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Re: LC Colonized, still need GE? [Re: SS32]
    #11193562 - 10/06/09 02:50 PM (8 years, 2 months ago)

Quote:

SS32 said:
Quote:

Doc_T said:
You can't tell anything by looking.
And is this the no-PC tek?




Correct.  I'll knock up a couple jars to make sure.




4 out of 4 failures with no PC... I'd say it's time to bite the bullet and buy a PC.


--------------------
You make it all possible. Doesn't it feel good?


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Invisibletruskool
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Re: LC Colonized, still need GE? [Re: Doc_T]
    #11193568 - 10/06/09 02:51 PM (8 years, 2 months ago)

yes if i could suggest anything to a noob is buy a pc even if your doing cakes BUY A PC


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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Invisiblefeelfunny
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Re: LC Colonized, still need GE? [Re: truskool]
    #11193575 - 10/06/09 02:53 PM (8 years, 2 months ago)

yep yep it will be needed if you stick with the hobby. i put mine off for over 6months and wish all the time i would have just done it to start with. would have defonetly made things easyer


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Offlinebeto
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Re: LC Colonized, still need GE? [Re: feelfunny]
    #11194420 - 10/06/09 05:10 PM (8 years, 2 months ago)

You really don't need a PC for making LC, you can use the microwave. I didn't have any contaminants so far.

Prepare the solution and microwave it in maximum potency for 3:30 minutes, u have to babysit it otherwise the liquid is going to explode. When u notice a violent bubbling pause it, then restart it. Once u open the microwave door quickly put the lid on the jar. Easy


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