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Offlinesmaerd
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Registered: 03/11/08
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First Wood-Lover Need Advice
    #11189671 - 10/05/09 09:57 PM (8 years, 2 months ago)

So I am working with Wine Caps(Strophaphoria R.A.). I just need some confirmation to know if what I am doing is stupid or if it's on track. Been doing my research but a little confirmation never hurt :wink:.

Here's what I have done so far:

Prepared several jars with standard PF tek substrate. inoculated from MS.

As an experiment:
I prepared several other jars just like the standard PF tek but I also put as many wet oak wood-chips as I could fit into the jars. Also inoculated from MS.


What I want to do is(here's where I need a little advice :laugh: ): Please tell me if any of this is unneccesary, bad practice, just won't work, comments, suggestions, etc.

1. Allow jars to fully colonize of course.
2. Cut pieces of the cake up(with sterile knife in a still air box even though it's FC'ed I don't risk things haha)
3. Mix cut pieces of cakes into sterile plastic trays(about 2-3 inches deep) with more PC'ed moist oak wood-chips
4. Immediately after filling the tray, I have sterile dry vermiculite I am going to case it with. I will probably case it with about a half inch of verm.
5. I am then going to cover the trays with foil or syran-wrap for or two weeks(to colonize the wood chips).
6. Place the trays into a shotgun FC, attempt to induce fruiting(mist, FAE, etc).

Would this tek work? I realize probably not to many people have experience with cultivating wine-caps I just mean in general. Would this work for most wood-lovers?

Thank you for your time!
:peace:


Edited by smaerd (10/05/09 10:08 PM)


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OfflinebioBot

Registered: 05/02/09
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Re: First Wood-Lover Need Advice [Re: smaerd]
    #11190940 - 10/06/09 01:00 AM (8 years, 2 months ago)

Your reasoning is sound, and I think you would succeed.

Another way you might find success is by using spawn
bags in place of trays. By tying them closed with string
or using metal clips before sterilization they can be
reopened in a glovebox or flowhood for transfer of
colonized grains.

It may provide less chance of contamination by limiting
the outside exposure to a higher degree... and lessening
the number of required transfers of material.  Also
during colonization of the wood chips, the convenience
of having everything quarantined in a transparent bag
is nice, not only for allowing one to see how they are
colonizing but also for the security of self-containment
compared to covered trays where there will be cracks to
outside air between the lid and body.

The layer of vermiculite on top is a good precaution with
using trays I think - but then there's the issue of plastic
wrap Vs. foil.  Foil is of course opaque, but prevents
moisture loss, and allows one the use of punched air-holes
if desired.  Plastic wrap is semi-permeable but allows
easier visibility when its not beaded with condensation.

Looking beyond the colonization stage, you'll have to
provide the environmental conditions to allow fruiting,
and this includes low temperatures in the 50-60F range
for primordia formation. Also they require a casing layer
of pasturized ( 130 - 140F ) organic material. Stamets
specifically recommends not exceeding 140F for an extended
period of time due to it causing extinction of the
beneficial microorganisms.


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Offlinesmaerd
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Re: First Wood-Lover Need Advice [Re: bioBot]
    #11191027 - 10/06/09 01:16 AM (8 years, 2 months ago)

I figure if I can get these trays to go then I will buy some spawn bags(before attempting another flush) and transfer some of the colonized wood-chips into sterilized bags :laugh:. They are so cheap and so useful.

I heard that they do like a colder temperature range to form primordia but once they get they are an inch or two then they like it to get a little warmer? I'm not that big of a perfectionist I figure that is optional and species dependent.

Hmmm pasturized organic matter? would that be something like coir, or dry fallen leaves. Is this relatively optional?

I trust Stamets he does amazing work.

Thank you for a nicely thought out post by the way!


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OfflinebioBot

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Posts: 275
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Re: First Wood-Lover Need Advice [Re: smaerd]
    #11204160 - 10/08/09 12:18 AM (8 years, 2 months ago)

You're right about the temperature variation, as far
I'm aware...  a bit warmer after primordia form into
mushrooms.

Organic matter for a casing material could probably be
just about any mostly non-nutritious material, even
potting soil or loamy dirt.


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Offlinesmaerd
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Registered: 03/11/08
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Re: First Wood-Lover Need Advice [Re: bioBot]
    #11204655 - 10/08/09 01:24 AM (8 years, 2 months ago)

Thanks again bio-bot, lots of good information :laugh:. Check your ratings.


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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