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Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon HEPA Filter, Lab Glassware, Laminar Flow Hood, Petri Dish, Pressure Cooker

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InvisibleMethadone
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Flow Hood Size for Tissue Culture
    #11183554 - 10/04/09 11:14 PM (8 years, 15 days ago)

I know we have some micropropagators lurking about.. To make a long story short, I have a bit of extra money and finally want to build a laminar flow hood for the secondary purpose of growing edible fungi, but primarily so I can begin some minor experimentation in tissue culture and develop some skill at it.. The flowhood size is the matter in which I'm unsure about.

I know that for mycology a 12" x 24" is more then optimal, giving you the ability to inoculate as many spawn bags as can fit into your average pressure cooker.. but will this size suffice for tissue culturing? I imagine it would, but I wanted to ask before I go in on any materials because of the possibility that theres some aspect that I'm not considering which could require more space then I would have.

I've figured out the rest of the aspects and have a blueprint which I could modify to any size easily, as well as all the tools needed. This will be a DIY deal which is why I need to begin looking for the appropriate parts(cheaply) now.

Thanks in advance for any help which can be offered.


-M


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InvisibleBrainiac
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Re: Flow Hood Size for Tissue Culture [Re: Methadone]
    #11184406 - 10/05/09 02:19 AM (8 years, 15 days ago)

Go with the 18x24 more room to work... Dont go cheap with the hepa and get a good(cheap?) pre-fiter.They will add more life to hepa...


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InvisibleMethadone
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Re: Flow Hood Size for Tissue Culture [Re: Brainiac]
    #11185612 - 10/05/09 10:43 AM (8 years, 15 days ago)

Yeah, I know to get the prefilter, by "cheap" I was referring to finding a deal on a true HEPA (99.99%@.3 microns) somewhere. I've read here that the numbers to use are at 1.2 Static pressure on the calculations.. Most blowers are rated at .8, but I was going to overshoot the needed CFM by 100 or so (@ .8) and then just block part of the blower off if needed with a piece of lumber (or similar, I've seen it done around here) until I get the proper airflow coming out.

Probably a good idea on the 18x24 as well, I'll start looking this Friday, so if anyone else has a suggestion/input please contribute.

Thanks Brainiac, I hear you raise Eisenia Fetida worms (Red wigglers) for composting. Hows that working out for you? I started a bin recently myself.

-M


Edited by Methadone (10/05/09 10:57 AM)


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InvisibleBig L
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Re: Flow Hood Size for Tissue Culture [Re: Methadone]
    #11187225 - 10/05/09 03:59 PM (8 years, 14 days ago)

I agree with 18". When you think about it 12" isn't really that much room. Other than that I have nothing to add, other than good luck.


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Offlinefelixhigh
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Methadone]
    #11187919 - 10/05/09 05:56 PM (8 years, 14 days ago)

This thread was moved from The Ethnobotanical Garden.

Reason:
Mushrooms don't belong to the garden.


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InvisibleBrainiac
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Re: Flow Hood Size for Tissue Culture (moved) [Re: felixhigh]
    #11188383 - 10/05/09 06:58 PM (8 years, 14 days ago)

As in Plant
Quote:

felixhigh said:
<img src='http://www.shroomery.org/forums/images/moved.gif'> This thread was moved from The Ethnobotanical Garden.

Reason:
Mushrooms don't belong to the garden.





Unless he was talking about Plant Tissue Culture...:facepalm:


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OfflineHorticulture
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Re: Flow Hood Size for Tissue Culture (moved) [Re: felixhigh]
    #11188386 - 10/05/09 06:58 PM (8 years, 14 days ago)

This is about tissue culturing plants if I read the topic correctly.  I think it should get sent back to the garden.  :shrug:


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InvisibleMankey
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Horticulture]
    #11188523 - 10/05/09 07:11 PM (8 years, 14 days ago)

Yup. Back to the garden.


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InvisibleMethadone
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Mankey]
    #11188620 - 10/05/09 07:21 PM (8 years, 14 days ago)

I told him to just leave it, I was thinking maybe I would get better answers here anyways?

Mods feel free to move again should you so desire. Wherever it belongs.

-M


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OfflineCactusdan
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Methadone]
    #11190612 - 10/06/09 12:03 AM (8 years, 14 days ago)

It was for tissue culturing of plant FH.


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OfflineRogerRabbitV
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Methadone]
    #11190837 - 10/06/09 12:40 AM (8 years, 14 days ago)

This thread was moved from Gourmet and Medicinal Mushrooms.

Reason:
I believe this was moved here by mistake.
RR


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Offlinefelixhigh
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Re: Flow Hood Size for Tissue Culture (moved) [Re: RogerRabbit]
    #11192334 - 10/06/09 10:49 AM (8 years, 14 days ago)

I know, I just figured he would get better answers there. :crazy:


FH


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InvisibleBig L
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Re: Flow Hood Size for Tissue Culture (moved) [Re: felixhigh]
    #11192345 - 10/06/09 10:52 AM (8 years, 14 days ago)

when I was doing research for flowhoods, I looked in Advanced Mycology. They know there shit in those parts.

*edit
don't know if you already had this info, but this is a great place to start.
here


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Edited by Big L (10/06/09 10:59 AM)


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InvisibleMethadone
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Big L]
    #11193155 - 10/06/09 01:44 PM (8 years, 13 days ago)

Thanks Big L, but yeah, I have all that info. I know everything I need to know to build it and estimate fan/filter size.. I was really just looking for some opinions from tissue culturists based on the sizes of the lab equipment they use.

Example: 12 x 24 is fine for spawn bags and petri dishes, but perhaps for tissue culturing I'll sometimes be needing a 2 foot graduated cylinder, or perhaps multiple large pyrex items in the area at once.

I was just curious as to see if all of the equipment required for Micropropagation would *fit* comfortably in front of a 12 x 24. It seems like it would, but some first hand advice is what I need here.

I need to know from a first hand source how comfortable a work area would be. I also need to know the limitations of using said work area as opposed to a larger one.

-M


Edited by Methadone (10/06/09 02:23 PM)


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InvisibleBig L
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Methadone]
    #11199915 - 10/07/09 01:20 PM (8 years, 12 days ago)

Meth,
keep us posted on your progress. I am very interested in what you decide.
I am probably going to be building one in the relatively near future.

thats would be the bees knees


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InvisibleMethadone
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Big L]
    #11200085 - 10/07/09 01:50 PM (8 years, 12 days ago)

Quote:

Big L said:
Meth,
keep us posted on your progress. I am very interested in what you decide.
I am probably going to be building one in the relatively near future.

thats would be the bees knees





This is going to take me awhile to find the parts. I have a great book on tissue culture + a few recipes for select cacti, but Im more of a hands on learner.. I cant get into the book until I can practice what I'm reading.

I'm looking for a hepa now, but its looking like the fan will cost me at least $250 or so..

Second hand blower fans seldom have the product # OR a spec sheet, so I would have to really luck out to find one thats appropriate.

I'll go with 18x24 or 24x24.

Anyone know if the filter thickness affects the static pressure requirements? Thats about the only thing I'm unclear on, it seems like it would, but I'm unsure. I see HEPA's between 5 and 7/8 inches all the way to nearly a foot thick.

I'll scour graingers for a fan once I find an appropriate filter.

-M


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InvisibleBig L
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Methadone]
    #11200185 - 10/07/09 02:05 PM (8 years, 12 days ago)

what formulas do you already have, if you don't mind me asking?

Quote:

Anyone know if the filter thickness affects the static pressure requirements? Thats about the only thing I'm unclear on, it seems like it would, but I'm unsure. I see HEPA's between 5 and 7/8 inches all the way to nearly a foot thick.





I would post that in Advanced Mycology. They will give you a good answer.


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Edited by Big L (10/07/09 02:11 PM)


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InvisibleBrainiac
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Big L]
    #11200799 - 10/07/09 03:49 PM (8 years, 12 days ago)

MF2418 24 x 18 x 5.8 inches $159.00 from Fungi.com

and this bower

This high-power, multi-purpose blower is the perfect choice for pushing air through our 24x18, 24x24 and 24x36 inch HEPA filters. Dual-voltage power supply operates at either 115 or 230V. Delivers 1,040 CFM at .8" static pressure. Draws 4.8/2.5 amps @115V or 7.3/3.7 amps @ 230V.
FAN831 $289.00


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InvisibleMethadone
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Re: Flow Hood Size for Tissue Culture (moved) [Re: Brainiac]
    #11200980 - 10/07/09 04:23 PM (8 years, 12 days ago)

The formulas I used were from the link you provided actually, BigL

2x2 hepa = 4 = 400CFM @ total static pressure (1.0-1.2 w/ prefilter).. right?

Factor in that the hepa is between .8 and 1.0 SP and the prefilters I have read are about .2, a 450-500CFM fan should be perfect for a 2x2 HEPA. I understand that it is best to over purchase them a bit stronger then needed because w/ most you can incorporate a built in speed control OR block part of the intake with something solid.

Thanks for the links Brain, but his prices are a little steep. Also, granting you I have done my math right, isnt that fan way too powerful for a 24x24 Hepa? Grant you it should lose some CFM's to the additional static pressure, but still, isnt that fan significantly more powerful then what I need?

Either I have done my math wrong or missed an aspect of this, so I guess its a good thing were getting into it now lol.


Edit: Or do you mean culturing formulas? Lmao..

I have one for Lophophora that was posted here a month or so ago and then I have everything that is in my book, but I havent seen any cacti.

Regardless, the basics of cacti TC seem to be areoles/chunks > MS media (+ vitamins in Lophs case) + BAP and another hormone or two. I can certainly attempt the same recipe for several types of cacti and modify it accordingly.. It gives me a foundation to start with at least, and thats another reason I want to get off mi culo and get the damned flow hood.. so I can begin to twink recipes like this.

-M


Edited by Methadone (10/07/09 05:50 PM)


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Mushrooms, Mycology and Psychedelics >> The Ethnobotanical Garden

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