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Invisiblemango man
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How to flame sterilize in a glovebox? (noob question)
    #11179959 - 10/04/09 01:45 PM (8 years, 4 months ago)

How do you guys flame sterilize the needle tip between injections in a glovebox?
I am always worried I'm going to set myself on fire because of the alcohol in the glovebox at the same time.
So is it better to flame the needle between injecting jars or alcohol swab the tip with alcohol instead?


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Offlinegotfungus
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11179975 - 10/04/09 01:49 PM (8 years, 4 months ago)

sounds like a bad idea to me. i pull my hands out every time i sterilize the needle between jars. i have a 100% rate for healthy myc.


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InvisibleDoc_T
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Re: How to flame sterilize in a glovebox? (noob question) [Re: gotfungus]
    #11179989 - 10/04/09 01:51 PM (8 years, 4 months ago)

If you have fire in the gb, don't have alcohol in there. Ever.


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Invisibleanonjon
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Re: How to flame sterilize in a glovebox? (noob question) [Re: Doc_T]
    #11180002 - 10/04/09 01:54 PM (8 years, 4 months ago)

Quote:

Doc_T said:
If you have fire in the gb, don't have alcohol in there. Ever.




This.


I found having a glass of alcohol in there worked. Dipped tool in alcohol in the toolbox, shake tip of tool, use tool, put tool in alcohol glass.


Edited by anonjon (10/04/09 01:55 PM)


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Offlinegotfungus
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Re: How to flame sterilize in a glovebox? (noob question) [Re: anonjon]
    #11180026 - 10/04/09 01:59 PM (8 years, 4 months ago)

Quote:

I found having a glass of alcohol in there worked. Dipped tool in alcohol in the toolbox, shake tip of tool, use tool, put tool in alcohol glass.


this is horrible advice. always flame sterilize between jars.


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Invisiblemango man
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Re: How to flame sterilize in a glovebox? (noob question) [Re: gotfungus]
    #11180038 - 10/04/09 02:02 PM (8 years, 4 months ago)

Quote:

gotfungus said:
Quote:

I found having a glass of alcohol in there worked. Dipped tool in alcohol in the toolbox, shake tip of tool, use tool, put tool in alcohol glass.


this is horrible advice. always flame sterilize between jars.




I actually tried this last batch I made and had a 50% contam rate.
Great advice from a friend that I will never take advice from again! lol.
I have never had so much contam in my life.


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me


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Offlinegotfungus
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11180047 - 10/04/09 02:05 PM (8 years, 4 months ago)

yeah just follow, and practice sterile procedure and it greatly reduces your chances of a contaminant.


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Invisiblemango man
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Re: How to flame sterilize in a glovebox? (noob question) [Re: gotfungus]
    #11180108 - 10/04/09 02:19 PM (8 years, 4 months ago)

Thank you fungus.
The advice (confirming what I thought) is much appreciated.
Sorry to be a bother everyone!


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me


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Offlinecloudsaregathering
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11180116 - 10/04/09 02:21 PM (8 years, 4 months ago)

Quote:

mango man said:
Thank you fungus.
The advice (confirming what I thought) is much appreciated.
Sorry to be a bother everyone!



everyone has been a bother at some point in there life...


--------------------

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Offlinesnesgamer
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Re: How to flame sterilize in a glovebox? (noob question) [Re: cloudsaregathering]
    #11180264 - 10/04/09 02:49 PM (8 years, 4 months ago)

i just use a bic lighter in glovebox.

I use an alcohol soaked paper towel in there also, never had any "setting myself on fire" issues


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OfflineSkaloser
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Re: How to flame sterilize in a glovebox? (noob question) [Re: snesgamer]
    #11180451 - 10/04/09 03:26 PM (8 years, 4 months ago)

You can swab it with alcohol and then flame it outside the box. If you flame the needle outside the box until the needle is glowing, you will have enough time to put it through the arm hole before it cools. I wouldn't want to ever mess around with fumes, still air, and fire...thats asking for trouble.


--------------------
This was unlike the story
it was written to be
I was riding its back
when it used to ride me.

And we were galloping manic
to the mouth of the source
we were swallowing panic
in the face of its force.


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Invisiblemango man
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Re: How to flame sterilize in a glovebox? (noob question) [Re: Skaloser]
    #11180495 - 10/04/09 03:35 PM (8 years, 4 months ago)

Is it really necessary to use alcohol if you flame sterilize?


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me


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OfflineSkaloser
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11180539 - 10/04/09 03:41 PM (8 years, 4 months ago)

no not really, flaming it will kill everything if you get it glowing, but I like to get the black stuff off the needle when knocking up multiple jars.


--------------------
This was unlike the story
it was written to be
I was riding its back
when it used to ride me.

And we were galloping manic
to the mouth of the source
we were swallowing panic
in the face of its force.


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Invisiblemango man
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Registered: 02/02/09
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Re: How to flame sterilize in a glovebox? (noob question) [Re: Skaloser]
    #11180564 - 10/04/09 03:47 PM (8 years, 4 months ago)

So theoretically wouldn't it be better to flame it in the glovebox and remove the needle from the box between jars and swab it?
Or am I completely wrong and it is better the other way around?


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me


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Invisibleanonjon
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11180606 - 10/04/09 03:56 PM (8 years, 4 months ago)

Theoretically you would have an alcohol lamp; they don't blacken the needle.

Can you just use an alcohol lamp inside the box?

Rubbing alcohol isn't that volatile ime.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


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OfflineSkaloser
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Re: How to flame sterilize in a glovebox? (noob question) [Re: mango man]
    #11180609 - 10/04/09 03:57 PM (8 years, 4 months ago)

You don't have to swab it if you don't want to, you can flame it in the box if you have let it air out sufficiently of fumes. Depending on the size of your glove box and the number of jars inside of it, it is easier I find to do it outside the box as you have more range of motion, space and you don't have to worry as much about fumes and open flames.


--------------------
This was unlike the story
it was written to be
I was riding its back
when it used to ride me.

And we were galloping manic
to the mouth of the source
we were swallowing panic
in the face of its force.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegotfungus
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Re: How to flame sterilize in a glovebox? (noob question) [Re: Skaloser]
    #11180642 - 10/04/09 04:04 PM (8 years, 4 months ago)

the purpose of the STALE air box is simple. you want the stale air. if you vent the box, obviously you let new air in from some where. just dont be lazy. there is no need to vent your STALE air box.


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