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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Scales

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OfflineGrzyby
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PDA agar mix help
    #11179307 - 10/04/09 11:12 AM (8 years, 2 months ago)

I have some powdered pda agar mix. No instructions. Iam looking to pour 20 dishes, how much should i use. I have a small postage scale, measuring spoons and a cheap digital food scale that might have grams.

Thanks

Any other advice too as this is my first attempt with agar.


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Offlinehoudinihar
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Re: PDA agar mix help [Re: Grzyby]
    #11179347 - 10/04/09 11:19 AM (8 years, 2 months ago)

Grzyby,
3 tablespoon per 500 cc should be about right (you will use less than 300 cc i assume, so you can dial down the quantity a little). use hot water, tap or R.O. to dissolve your agar, then pour into at least a 700 cc glass or polypropylene container, place a foil cap over it and PC for 45-60 minutes at 250 degree, 15psi with enough water. let cool enough to touch and pour your plates in the hood or box.
let cool to set up the agar. some poeple use a poly fiberfil insert under the foil.
are your plates 100 mm?

houdinihar


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OfflineGrzyby
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Re: PDA agar mix help [Re: houdinihar]
    #11179377 - 10/04/09 11:24 AM (8 years, 2 months ago)

yes 100mm, i have a 500ml nalgene flask. How do i measure 500 cc? Sorry , very new to this.


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OfflineSOUTHERN
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Re: PDA agar mix help [Re: Grzyby]
    #11179511 - 10/04/09 11:52 AM (8 years, 2 months ago)

I am using fungi perfecti malt extract agar. i take one level table spoon to 145 ml of water .this will pour like 7-9 depending on how thin u pour it. I do my dishes different than they recommend i mix my agar and go ahead and pour it in the dishes then i stack them on a jar or two to hold them outta the water in the pc. then i cook and remove the petris once it is kewl enough i can get into it with some oven mitts . i wrap them in foil and put them in the fridge. probley ass backwards but its always worked for me


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Offlinesolumvita
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Re: PDA agar mix help [Re: SOUTHERN]
    #11180167 - 10/04/09 02:31 PM (8 years, 2 months ago)

Southern you must be using glass petri dishes or some really expensive plastic ones?

Gryzby try not too pour the agar when it is too hot as it will cause a lot of condensation on the lids of the dishes.

Wait until it is cool enough to handle without a glove but not cold so that it has started solidifying.


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OfflineSOUTHERN
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Re: PDA agar mix help [Re: solumvita]
    #11181678 - 10/04/09 07:08 PM (8 years, 2 months ago)

GLASS  there good ones until ya drop it.. lol


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OfflineGrzyby
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Re: PDA agar mix help [Re: SOUTHERN]
    #11183200 - 10/04/09 10:26 PM (8 years, 2 months ago)

first attempt was not very good. I poured too hot, which resulted in condensation and also spilled a bunch in the glove box. It was a good learning experience though.


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OfflineGrzyby
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Re: PDA agar mix help [Re: Grzyby]
    #11183213 - 10/04/09 10:27 PM (8 years, 2 months ago)

Why is the condensation negative? Increased chance of contamination??


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OfflineSOUTHERN
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Re: PDA agar mix help [Re: Grzyby]
    #11183235 - 10/04/09 10:30 PM (8 years, 2 months ago)

it makes it hard to see in good.


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InvisibleImorph
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Re: PDA agar mix help [Re: SOUTHERN]
    #11194251 - 10/06/09 04:42 PM (8 years, 2 months ago)

Hey Grzyby maybe i can help a little ? using Tablespoons and teaspoons,
1 Tbsp = 3 tsps

dry measure for white sugar is  per level 1 Tbsp = 14 grams and  1 level tsp = 4.6 grams 


Sporewrks
20 petri dishes add 20 grams of MEA to 500 ml or  40 grams/liter of H2O

That's about 1.5 Tbsp Pre-mixed media powder per 20  petris  to  half liter H2O with amounts doubled for 40 petris to full qrt/liter.

Fungi Perf
50 grams of media (their blend) are recommended per liter of water. 

There's a bit of extra media added  but  3 Tbsp + 2 tsp = 51.2 grams.


you can also go ahead if you got the space and pc as many petris per pc load as you can. ..20 or 40 saving the extras for next agar work by wrapping them in foil ...either individually or

to gether , placing the extras in a clean plastic bag or area until needed...then no need to heat up pc just uncover the extras inside glovebox after mild box spraying during next agar work...i think they should keep since your sterilizing tek is MOSTLY (!@#$^) contam free : )


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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Scales

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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