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Offlinejust me
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couple questionable ms petris
    #11177174 - 10/03/09 10:27 PM (8 years, 2 months ago)

this is my first time starting from MS on agar
a friend says this looks normal, but its got me freakin out

Falbino:


Shooting Star Cube:


i guess just all the tiny obvious colonies of something has me sceptical.

if anyone can say guaranteed that some of that is good myc growth, whats my next step? transfering from one colony to a new petri dish? etc.

i'll get some pictures in a day or so when growth will be more pronounced. and maybe a shot from around the edges. the myc climbing the glass looks like cube myc, just very thin


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Offlinejust me
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Re: couple questionable ms petris [Re: just me]
    #11177187 - 10/03/09 10:29 PM (8 years, 2 months ago)

the first one looks weird because the agar mex was poured too shallow and it cracked like thin ice in the center...or something


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Invisiblebadman
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Re: couple questionable ms petris [Re: just me]
    #11177211 - 10/03/09 10:32 PM (8 years, 2 months ago)

Top one looks alright but its difficult to tell. Just transfer the clean good looking myc to new dishes or jars.

Where did you get the falbino? ive been looking for this for a while.


Edited by badman (10/03/09 10:49 PM)


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Invisibledpga
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Re: couple questionable ms petris [Re: just me]
    #11177223 - 10/03/09 10:33 PM (8 years, 2 months ago)

looks sorta like mine i just started agar also


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Invisiblemyco_mikey_mo
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Re: couple questionable ms petris [Re: just me]
    #11177228 - 10/03/09 10:33 PM (8 years, 2 months ago)

looks good, but around the edge looks a little weird. the first pic does look healthy though. and YES some of that myc is healthy. you might want to do a transfer if your getting freaked out, but personally i would let it be


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Invisibleratdog
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Re: couple questionable ms petris [Re: myco_mikey_mo]
    #11177302 - 10/03/09 10:44 PM (8 years, 2 months ago)

looks fine

pour thicker next time

mine look like this




next time catch it sooner.... and take the biggest whitest spots and transfer 4 to a new dish. once they grow out then pull from the edge and do separate plates.

on your 1st transfer "the 4" draw a X on the bottom and number 1-4 so when you do your 2nd transfer you can number plates 1-4 etc.

looking good man


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some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:


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Offlinejust me
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Re: couple questionable ms petris [Re: ratdog]
    #11178337 - 10/04/09 02:12 AM (8 years, 2 months ago)

thanks
and thanks for some compaison pics

i just got some new petris so ill be takin all kinds of samples

$70 for case of 500 100x15mm delivered to hawaii :shrug: not too shabby


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Offlinejust me
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Re: couple questionable ms petris [Re: just me]
    #11178637 - 10/04/09 04:48 AM (8 years, 2 months ago)

mannnnnn

i dont know if i wanna waste my time.

i still cant convince myself theyre cube mycelium

i completely forgot that this isnt my first ms on agar.

my first ones look waaaaaaaay different...way mo betta?

pura vida, texas,(BOTH ACTUAL FIRST MULTISPORE>AGAR) and a maz clone for fun


so im kinda scared to toy around with what i have here now. more ppl tell me please its ok :lol:


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Edited by just me (10/04/09 05:14 AM)


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Invisibletruskool
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Re: couple questionable ms petris [Re: just me]
    #11178653 - 10/04/09 04:58 AM (8 years, 2 months ago)

I would put money that that is cube myc:thumbup:


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Invisibleratdog
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Re: couple questionable ms petris [Re: truskool]
    #11179015 - 10/04/09 10:00 AM (8 years, 2 months ago)

yes it does look different from mine but you should know what you put on the plate. mine is plut.pop oyster but its still a mushroom and they should grow somewhat the same. and look close to each other.

ms seems to be the way to go for multi strains on one plate and the sooner you can separate them the easer it is. once they get to developed they combine and you have to grow them out adding another step.

some of the pics above do not look like ms but rather isolation attempts from other plates not the original spores.

but hay what do i know you might have been doing a search for a mushroom in your area with the use of only one spore with a hard on.


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some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:


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Offlinebeto
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Re: couple questionable ms petris [Re: ratdog]
    #11179230 - 10/04/09 10:58 AM (8 years, 2 months ago)

Take a look at mine:



I have about ten petri dishes that are looking like this, i just threw it all away and started over. On this pic on the right u can see the sectoring right? I did a transfer from each sector to individual dishes, but still that fucking cotton shit growing in all of them.

I wouldn't scratch spores right on to agar, make a LC first, you want a little bit of spores, not a lot.


Edited by beto (10/04/09 11:01 AM)


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Invisibleratdog
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Re: couple questionable ms petris [Re: beto]
    #11179629 - 10/04/09 12:24 PM (8 years, 2 months ago)

Quote:

beto said:
Take a look at mine:



I have about ten petri dishes that are looking like this, i just threw it all away and started over. On this pic on the right u can see the sectoring right? I did a transfer from each sector to individual dishes, but still that fucking cotton shit growing in all of them.

I wouldn't scratch spores right on to agar, make a LC first, you want a little bit of spores, not a lot.





what type of myco is it? and i seem to get better results the other way around. i might be wrong but i think the ick grows interlaced in the myco in lc and when you make plates its harder to isolate. once isolated i go to lc and i am sure it is clean. i would not even hesitate to go from lc to lc after agar work.

sorry for the lrg amount of posts here but i am in the middle of this and am looking for info too. just thought i might pass on my observations too.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:


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Re: couple questionable ms petris [Re: ratdog]
    #11179643 - 10/04/09 12:28 PM (8 years, 2 months ago)

cobweb?


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Invisibleratdog
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Re: couple questionable ms petris [Re: botanisthype]
    #11179650 - 10/04/09 12:31 PM (8 years, 2 months ago)

yep i think so too.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:


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Offlinejust me
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Re: couple questionable ms petris [Re: ratdog]
    #11180086 - 10/04/09 02:15 PM (8 years, 2 months ago)

Quote:

ratdog said:
sorry for the lrg amount of posts here but i am in the middle of this and am looking for info too. just thought i might pass on my observations too.




its appreciated. thank you

im going to do some transfering today. oh man, a bunch of fun project for the future!!!

i would also rather have healthy agar growth > LC


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."


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Re: couple questionable ms petris [Re: just me]
    #11182239 - 10/04/09 08:30 PM (8 years, 2 months ago)

ok. tranfering done.

i have a lot of genetics work going on right now...all started around 9/30

PE - print to agar - clean growth - wedge taken to fresh agar 10/4
Miracle PE - colonized grain to agar - clean growth - wedge to fresh agar 9/30 - clean growth - 2 LC's from grain agar - one awaits testing
Argentina - print/spores to agar
Shooting Star - print to syringe - syringe to agar - clean growth - myc water strip from agar 10/4 - full syringe of agar LC
LK - print to syringe - syringe to agar 9/30 - contam? - remainder of syringe used on brf tester cakes
Pan Cyan - clone from wild to agar - suspected clean growth - wedge taken to fresh agar 10/4
Shiitake - clone from store to agar 9/26 - some clean some contam - wedge transfered from clean to fresh agar 9/30 -
OR - print/spores to agar 10/4
Falbino - print to syringe - syringe to agar 9/26 - some clean growth - wedge transfered to fresh agar 10/4 - LC started 10/4
PB - print to syringe - syringe to agar - contam - remainder of syringe used for brf tester cakes - one LC made 10/4
Maz/Mal - print/spores to LC 10/4

i'll just post updates as it progresses

put some good vibez into this yall, i wanna grow most of these out, really badly


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Invisiblebadman
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Re: couple questionable ms petris [Re: just me]
    #11182275 - 10/04/09 08:35 PM (8 years, 2 months ago)

where did you get the falbino from?


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Offlinejust me
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Re: couple questionable ms petris [Re: badman]
    #11182280 - 10/04/09 08:36 PM (8 years, 2 months ago)

from the gettin place

ill give away prints if you help it grow


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Re: couple questionable ms petris [Re: just me]
    #11182320 - 10/04/09 08:43 PM (8 years, 2 months ago)

Help grow it ahahahah you have ask. Id stop all my pan/oyster projects for this cube and this one only.


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InvisibleReadBeforePosting
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Re: couple questionable ms petris [Re: badman]
    #11182373 - 10/04/09 08:48 PM (8 years, 2 months ago)

less is more w/MS on agar (MS inoculate not agar)


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