Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Advice? Just got 20 dishes and some agar. (pic added)
    #11170878 - 10/02/09 04:46 PM (14 years, 5 months ago)

ok i have the agar and dishes and now i need to make the dishes up..

a few questions.
#1 i have read agar techs and they say pc the lot then pour into dishes.. why cant i prepare the agar, pour into dishes the pc the dishes?

#2 i was thinking about adding grain water as the nutes for the agar. i was thinking like 5ml grain water to 100ml of water, or 25 ml of grain water for 500ml.

#3 i have molasses, is it better to use that as nutes?

#4 any advice of favorite teks?


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Edited by Slimz (10/02/09 05:51 PM)

Extras: Filter Print Post Top
Invisibletruskool Happy Birthday!
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11170929 - 10/02/09 04:55 PM (14 years, 5 months ago)

IME when I did agar before I poured the dishes then pc'd but I was using small mason jars so ......
Why dont you use honey or something I would use the molasses before the grain water
http://www.shroomery.org/forums/showflat.php/Number/11095532
nomnomnom coloured agar is lfun


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Invisiblefeelfunny
I am you
Male User Gallery


Folding@home Statistics
Registered: 03/11/09
Posts: 8,747
Loc: South
Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11170991 - 10/02/09 05:05 PM (14 years, 5 months ago)

nice link drop:wink:


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
Invisibletruskool Happy Birthday!
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Advice? Just got 20 dishes and some agar. [Re: feelfunny]
    #11171012 - 10/02/09 05:08 PM (14 years, 5 months ago)

Quote:

feelfunny said:
nice link drop;)



nomnomnom
Love that agar I am making some red agar right now!!!!  I'll post in the journal later.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11171144 - 10/02/09 05:39 PM (14 years, 5 months ago)

well i decided to just go for it..

i added 10ml grain water and 1/8tsp molasses and 12tsp agar to half a cup water

brought that to a heavy boil

poured my 6 dishes (last one is thin) and then PCd them for a few min. letting them cool now, ill tell ya how it goes.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
Invisibletruskool Happy Birthday!
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171163 - 10/02/09 05:44 PM (14 years, 5 months ago)

Looking forward to the results.  Used both water(grain) and molassess.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11171167 - 10/02/09 05:45 PM (14 years, 5 months ago)

yes. i figured i would give the myc some variety


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171185 - 10/02/09 05:51 PM (14 years, 5 months ago)

here are some pics of the last dish.. i did not PC this, its kinda a control.



--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171234 - 10/02/09 06:02 PM (14 years, 5 months ago)

question.. would ii be stupid to put a silicone inoc port on the top of a tray? small hole, dab of RTV, that way i dont have to open the tray.. then i could use peroxide on my other trays, the ported tray would be for starting from spore and the others would be for live transfers.. make sense?


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175495 - 10/03/09 02:43 PM (14 years, 5 months ago)

bump.
still looking for an answer to that question..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
Invisibletruskool Happy Birthday!
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175519 - 10/03/09 02:48 PM (14 years, 5 months ago)

Maybe cause ppl dont understand what you are asking.  I know I am totally lost.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11175540 - 10/03/09 02:53 PM (14 years, 5 months ago)

ok.. if i have a petri dish with a small hole in the top and an some silicone covering the hole and i fill it with nutrient agar without peroxide and PC it, then i can inject spores from a syringe without ever opening it.

then i use peroxide in the agar of the rest of my trays to thwart contam. once i have my live myc, i can transfer from the non peroxide tray to the peroxide trays..

does that sound like a good idea?


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
Offlinepftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175756 - 10/03/09 03:33 PM (14 years, 5 months ago)

glass petri dishes?


Edited by pftek (10/03/09 03:35 PM)

Extras: Filter Print Post Top
Invisiblebotanisthype
P$ylocibe$ounDW@vE!
Male User Gallery


Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR
Re: Advice? Just got 20 dishes and some agar. [Re: pftek]
    #11175835 - 10/03/09 03:45 PM (14 years, 5 months ago)

basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol

Extras: Filter Print Post Top
Invisiblefeelfunny
I am you
Male User Gallery


Folding@home Statistics
Registered: 03/11/09
Posts: 8,747
Loc: South
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11176420 - 10/03/09 05:47 PM (14 years, 5 months ago)

but are they glass petri dishes?  you could use jars and do that put the RTV on it and PC that way.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

Extras: Filter Print Post Top
InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
Male User Gallery


Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: Advice? Just got 20 dishes and some agar. [Re: botanisthype]
    #11176954 - 10/03/09 07:39 PM (14 years, 5 months ago)

they are plastic..
Quote:

botanisthype said:
basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...




im gonna try it once i get my agar tek working.
need to work with it some and get used to the procedures.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

Extras: Filter Print Post Top
Offlinepftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11176987 - 10/03/09 07:46 PM (14 years, 5 months ago)

Quote:

Slimz said:
they are plastic..
Quote:

botanisthype said:
basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...




im gonna try it once i get my agar tek working.
need to work with it some and get used to the procedures.




at how many PSI and for how long?

As you can see from my pic of petri dishes it didn't turn out so well.

Extras: Filter Print Post Top
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Advice? Just got 20 dishes and some agar. [Re: pftek]
    #11177048 - 10/03/09 07:59 PM (14 years, 5 months ago)

Looks like your plates cant handle a PCing

Edited by badman (10/03/09 08:26 PM)

Extras: Filter Print Post Top
Offlinecharvo
JOURNEYMAN
Male


Registered: 03/09/03
Posts: 654
Loc: In The Hand Of The World
Last seen: 9 years, 11 months
Re: Advice? Just got 20 dishes and some agar. [Re: badman]
    #11177143 - 10/03/09 08:22 PM (14 years, 5 months ago)

my comment would be that using a syringe into inoculate the plate isn't rly the standard way of starting a multispore to agar. Its totally possible to do that.One reason for using the inoculation loop and laying down spores in a streaking pattern is you'll you have better visible sectoring to isolate form. Also standing water form a syringe may welcome some bacteria.


--------------------

Extras: Filter Print Post Top
Invisibleratdog
wild capture trader
Male User Gallery


Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
Re: Advice? Just got 20 dishes and some agar. [Re: charvo]
    #11177241 - 10/03/09 08:36 PM (14 years, 5 months ago)

ok so pc might not have worked i bet.

the hole in the top is a cool idea but you will still need glove box to work in or flow hood " working on build now... if you got an extra filter i need it" to do you transfer to h202 plates. not everything in the air is just spores some float after germination.........go figure lol.

i boil my ag first then pc and pour in my hood. cool in my hood and have it all set up for transfers before i pour so i do not oped till plates are done. then i incubate them and as soon as i see contam i boot them.


grain water is a good idea too but 5 mil seems low. and i would filter the shit out of it too.

nuts?  well iuse malt ext. and corn sugar


and as fare a tek pick one and do what works for you ..... ibet if you do it 10 times you will make changes and it will work better.

i am on my 150th plate so i just started.... LOL!


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Re: Petri Dishes & Agar Q's Placebo 4,007 5 12/07/99 10:00 AM
by Anonymous
* The Jebus Dehydrator Tek v1.0 (NEW PICS ADDED)
( 1 2 all )
jebus2000 6,541 22 09/21/01 06:17 PM
by Chemical_Smile
* Re: My HORRIBLE accident with agar (pic) Coma of Souls 1,275 2 01/15/00 10:46 AM
by camel
* how many dishes will i get form 250 grams of agar lostagain 988 8 10/01/02 02:05 PM
by lostagain
* Using Agar medium directly from spore print?? djveiox 7,210 17 12/07/23 03:30 PM
by Screwup
* Agar and growing that white stuff! pj541 2,554 5 10/15/01 04:06 PM
by pj541
* Antibac Agar, DIY! superduper 4,879 13 12/04/02 02:20 PM
by superduper
* B+cake Pics Need Help LucidChu3 1,755 6 01/24/02 08:38 PM
by fresh357

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,084 topic views. 20 members, 126 guests and 118 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.008 seconds on 14 queries.