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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Petri Dish

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InvisibleSlimz
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Advice? Just got 20 dishes and some agar. (pic added)
    #11170878 - 10/02/09 06:46 PM (8 years, 2 months ago)

ok i have the agar and dishes and now i need to make the dishes up..

a few questions.
#1 i have read agar techs and they say pc the lot then pour into dishes.. why cant i prepare the agar, pour into dishes the pc the dishes?

#2 i was thinking about adding grain water as the nutes for the agar. i was thinking like 5ml grain water to 100ml of water, or 25 ml of grain water for 500ml.

#3 i have molasses, is it better to use that as nutes?

#4 any advice of favorite teks?


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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Edited by Slimz (10/02/09 07:51 PM)


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Invisibletruskool
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11170929 - 10/02/09 06:55 PM (8 years, 2 months ago)

IME when I did agar before I poured the dishes then pc'd but I was using small mason jars so ......
Why dont you use honey or something I would use the molasses before the grain water
http://www.shroomery.org/forums/showflat.php/Number/11095532
nomnomnom coloured agar is lfun


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Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11170991 - 10/02/09 07:05 PM (8 years, 2 months ago)

nice link drop:wink:


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Re: Advice? Just got 20 dishes and some agar. [Re: feelfunny]
    #11171012 - 10/02/09 07:08 PM (8 years, 2 months ago)

Quote:

feelfunny said:
nice link drop;)



nomnomnom
Love that agar I am making some red agar right now!!!!  I'll post in the journal later.


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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11171144 - 10/02/09 07:39 PM (8 years, 2 months ago)

well i decided to just go for it..

i added 10ml grain water and 1/8tsp molasses and 12tsp agar to half a cup water

brought that to a heavy boil

poured my 6 dishes (last one is thin) and then PCd them for a few min. letting them cool now, ill tell ya how it goes.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
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:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Invisibletruskool
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171163 - 10/02/09 07:44 PM (8 years, 2 months ago)

Looking forward to the results.  Used both water(grain) and molassess.


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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11171167 - 10/02/09 07:45 PM (8 years, 2 months ago)

yes. i figured i would give the myc some variety


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171185 - 10/02/09 07:51 PM (8 years, 2 months ago)

here are some pics of the last dish.. i did not PC this, its kinda a control.



--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11171234 - 10/02/09 08:02 PM (8 years, 2 months ago)

question.. would ii be stupid to put a silicone inoc port on the top of a tray? small hole, dab of RTV, that way i dont have to open the tray.. then i could use peroxide on my other trays, the ported tray would be for starting from spore and the others would be for live transfers.. make sense?


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175495 - 10/03/09 04:43 PM (8 years, 2 months ago)

bump.
still looking for an answer to that question..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Invisibletruskool
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175519 - 10/03/09 04:48 PM (8 years, 2 months ago)

Maybe cause ppl dont understand what you are asking.  I know I am totally lost.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: truskool]
    #11175540 - 10/03/09 04:53 PM (8 years, 2 months ago)

ok.. if i have a petri dish with a small hole in the top and an some silicone covering the hole and i fill it with nutrient agar without peroxide and PC it, then i can inject spores from a syringe without ever opening it.

then i use peroxide in the agar of the rest of my trays to thwart contam. once i have my live myc, i can transfer from the non peroxide tray to the peroxide trays..

does that sound like a good idea?


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11175756 - 10/03/09 05:33 PM (8 years, 2 months ago)

glass petri dishes?



Edited by pftek (10/03/09 05:35 PM)


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Invisiblebotanisthype
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Re: Advice? Just got 20 dishes and some agar. [Re: pftek]
    #11175835 - 10/03/09 05:45 PM (8 years, 2 months ago)

basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...


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Invisiblefeelfunny
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11176420 - 10/03/09 07:47 PM (8 years, 2 months ago)

but are they glass petri dishes?  you could use jars and do that put the RTV on it and PC that way.


--------------------
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If the sky is falling, don't look up!  :abduction:

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InvisibleSlimz
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Re: Advice? Just got 20 dishes and some agar. [Re: botanisthype]
    #11176954 - 10/03/09 09:39 PM (8 years, 2 months ago)

they are plastic..
Quote:

botanisthype said:
basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...




im gonna try it once i get my agar tek working.
need to work with it some and get used to the procedures.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepftek
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Re: Advice? Just got 20 dishes and some agar. [Re: Slimz]
    #11176987 - 10/03/09 09:46 PM (8 years, 2 months ago)

Quote:

Slimz said:
they are plastic..
Quote:

botanisthype said:
basically he wants to add healing ports to the plastic petris...which could be possible ...so he can go from syringe to agar......sounds good enough that i would try it...




im gonna try it once i get my agar tek working.
need to work with it some and get used to the procedures.




at how many PSI and for how long?

As you can see from my pic of petri dishes it didn't turn out so well.


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Re: Advice? Just got 20 dishes and some agar. [Re: pftek]
    #11177048 - 10/03/09 09:59 PM (8 years, 2 months ago)

Looks like your plates cant handle a PCing


Edited by badman (10/03/09 10:26 PM)


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Re: Advice? Just got 20 dishes and some agar. [Re: badman]
    #11177143 - 10/03/09 10:22 PM (8 years, 2 months ago)

my comment would be that using a syringe into inoculate the plate isn't rly the standard way of starting a multispore to agar. Its totally possible to do that.One reason for using the inoculation loop and laying down spores in a streaking pattern is you'll you have better visible sectoring to isolate form. Also standing water form a syringe may welcome some bacteria.


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Invisibleratdog
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Re: Advice? Just got 20 dishes and some agar. [Re: charvo]
    #11177241 - 10/03/09 10:36 PM (8 years, 2 months ago)

ok so pc might not have worked i bet.

the hole in the top is a cool idea but you will still need glove box to work in or flow hood " working on build now... if you got an extra filter i need it" to do you transfer to h202 plates. not everything in the air is just spores some float after germination.........go figure lol.

i boil my ag first then pc and pour in my hood. cool in my hood and have it all set up for transfers before i pour so i do not oped till plates are done. then i incubate them and as soon as i see contam i boot them.


grain water is a good idea too but 5 mil seems low. and i would filter the shit out of it too.

nuts?  well iuse malt ext. and corn sugar


and as fare a tek pick one and do what works for you ..... ibet if you do it 10 times you will make changes and it will work better.

i am on my 150th plate so i just started.... LOL!


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so here is a video or two or three for you guys:rolleyes:


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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Petri Dish

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