|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Using agar to inoculate WBS
#11166091 - 10/01/09 08:37 PM (14 years, 5 months ago) |
|
|
i don't know if their is mold spores on the print. just saying if their were mold spores on the print. would taking from the edge make sure i got none? what if I transferred it a few time from the edge and still see no mean green?
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166097 - 10/01/09 08:39 PM (14 years, 5 months ago) |
|
|
You would have to do multiple transfers on agar to clean up a contamed print
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Re: Using agar to inoculate WBS [Re: Shea25]
#11166131 - 10/01/09 08:45 PM (14 years, 5 months ago) |
|
|
Quote:
Shea25 said: You would have to do multiple transfers on agar to clean up a contamed print
interesting. and how would you know if it's contaminated or if it's a clean print? just tell by looking?
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166141 - 10/01/09 08:46 PM (14 years, 5 months ago) |
|
|
Can't tell by looks got to test it out on a agar dish or innoc up 1 jar with it
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Re: Using agar to inoculate WBS [Re: Shea25]
#11166189 - 10/01/09 08:54 PM (14 years, 5 months ago) |
|
|
Quote:
Shea25 said: Can't tell by looks got to test it out on a agar dish or innoc up 1 jar with it
okay inoculated jar. it all turns white. i've had jars do this that had contams. sometimes it too a jar g2g transfer to notice. sometimes even another.
If I had a microscope could i cut a section off and look to find mold spores?
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166195 - 10/01/09 08:55 PM (14 years, 5 months ago) |
|
|
Sure if you have a nice microscope you should see the mold spores
|
feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
|
Re: Using agar to inoculate WBS [Re: Shea25]
#11166358 - 10/01/09 09:21 PM (14 years, 5 months ago) |
|
|
i only incoulate with agar it is so much faster this was 18 hours later
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Re: Using agar to inoculate WBS [Re: feelfunny]
#11166435 - 10/01/09 09:32 PM (14 years, 5 months ago) |
|
|
Quote:
feelfunny said: i only incoulate with agar it is so much faster this was 18 hours later
how do you know that's not mold you are growing in conjunction to mycelium?
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166438 - 10/01/09 09:32 PM (14 years, 5 months ago) |
|
|
He would have seen the mold on the dish of agar
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Re: Using agar to inoculate WBS [Re: Shea25]
#11166516 - 10/01/09 09:44 PM (14 years, 5 months ago) |
|
|
lol what? you just said you can't tell by looking at the agar? if the agar is white and looks like mycelium it's not contaminated?
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166548 - 10/01/09 09:48 PM (14 years, 5 months ago) |
|
|
well here is how it works you knock up some agar with whatever (spore, tissue whatever)
then you grow it out.. the mushroom myc will be ropey and rhyzomorphic you then tahe a sample of the good MYC and transfer to a new agar plate
then once that grows out (contam free) you have a good source to inoc your jars with.
you can go even further with the plates and transfering but more on that later.
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166622 - 10/01/09 09:57 PM (14 years, 5 months ago) |
|
|
No I didnt this is what you wrote
Quote:
interesting. and how would you know if it's contaminated or if it's a clean print? just tell by looking?
then I said
Can't tell by looks got to test it out on a agar dish or innoc up 1 jar with it
I was talking about the print like you said
|
pftek
1337
Registered: 08/18/08
Posts: 832
Last seen: 13 years, 5 months
|
Re: Using agar to inoculate WBS [Re: Slimz]
#11166846 - 10/01/09 10:29 PM (14 years, 5 months ago) |
|
|
Quote:
Slimz said: well here is how it works you knock up some agar with whatever (spore, tissue whatever)
then you grow it out.. the mushroom myc will be ropey and rhyzomorphic you then tahe a sample of the good MYC and transfer to a new agar plate
then once that grows out (contam free) you have a good source to inoc your jars with.
you can go even further with the plates and transfering but more on that later.
i see. so it's not a good idea to use the first agar dish but a second or third transfer before inoculation.
|
Slimz
.-~*´`*·~-experience-~*´`*·~-.
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
|
Re: Using agar to inoculate WBS [Re: pftek]
#11166863 - 10/01/09 10:32 PM (14 years, 5 months ago) |
|
|
by George i think he's got it..
yes the transfers are to ensure no contam..
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! ! me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
|
Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
|
Re: Using agar to inoculate WBS [Re: Slimz]
#11166869 - 10/01/09 10:33 PM (14 years, 5 months ago) |
|
|
lol thats what I was trying to say at the beginning about transfers and what not
|
ratdog
wild capture trader
Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
|
Re: Using agar to inoculate WBS [Re: Shea25]
#11166996 - 10/01/09 10:54 PM (14 years, 5 months ago) |
|
|
how do
so you got spores from print........ you make a tube with part of the print. and put a drop from the tub onto a mda plate. wait 1+ week and you get this.....
can you see all the little dots? each one is a different strain.....or what ever you want to call it..... they each come from 2 spores linked together.
now you remove the biggest/fastest growing ones and put them on new plates. this is were you cross your fingers and hope they grow out nice.
if they are con tamed then remove a section from the plate that has no contam and put it on a new plate. and repeat if you have to.
now you can take some myco from your uncontamed isolated plate and make a lc. which you inject into grain jars.
once they grow you can do g2g. and this is the point you want to be at....... i mean its the reason for all the agar work anyway right.
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
|
Jitsu
JKD Love
Registered: 11/12/08
Posts: 1,073
|
Re: Using agar to inoculate WBS [Re: pftek]
#11167824 - 10/02/09 01:27 AM (14 years, 5 months ago) |
|
|
Quote:
pftek said: i don't know if their is mold spores on the print. just saying if their were mold spores on the print. would taking from the edge make sure i got none? what if I transferred it a few time from the edge and still see no mean green?
No. Taking from the edge of the print won't make a difference.
Trichoderma and other Molds often appear bright white and can appear to be mycelium to the untrained eye. After a few times working with mycelium on agar you will be able to easily tell if your dish is contaminated. Mold spores aren't common on prints from indoor grows. However if you are using wild prints, mold spores are usually abundant. This has been my experience at least.
Bacteria is the most common contamination i've seen with hobby growers.
Cleaning up a contaminated print isn't even a pain if you're working with Cubensis.
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
|
|