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Amazon Shop: ½ Pint Jars, 10cc Syringe, Mycobags

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InvisibleTacoHerder
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LC question
    #11165730 - 10/01/09 09:15 PM (8 years, 1 month ago)

So i have this jar of LCfrom mycopath.  Here is what it says to do
Quote:

Instructions: Just insert needle into injection site and hold plunger so that it does not inject the entire syringe into the jar, only add 1-2cc’s of spores or culture into jar. Next you will want to inject an air port syringe into injection site and allow the vacuum to be released and introduce oxygen into the jar (make sure the lid is on tight). Another method of oxygen introduction is to hold the needle of an empty sterile syringe over a torch flame, and slowly inject air into the syringe (this air should be sterile since its being held over the flame). Then inject this air into the jar, repeat until vacuum is released. Add a minimum of 10cc’s of air each week to keep the culture alive. Simply incubate to proper temperature and watch your culture grow. 500ml. Guaranteed to be sterile and contaminate free.




I thought i was suppose to do it the other way around. inject the needle full of air to release the vacuum.  Are those directions correct?


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Re: LC question [Re: TacoHerder]
    #11165769 - 10/01/09 09:21 PM (8 years, 1 month ago)

I'd release the vacuum first, myself.


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InvisibleTacoHerder
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Re: LC question [Re: Doc_T]
    #11165800 - 10/01/09 09:28 PM (8 years, 1 month ago)

:shrug:Thats what i always figured


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Re: LC question [Re: TacoHerder]
    #11165852 - 10/01/09 09:42 PM (8 years, 1 month ago)

It's your LC, do it the way you think is right.


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InvisibleTacoHerder
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Re: LC question [Re: Doc_T]
    #11165861 - 10/01/09 09:44 PM (8 years, 1 month ago)

Ive read quit a few stories of ppl losing the whole syringe cause they didnt release the vacuum first.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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Re: LC question [Re: TacoHerder]
    #11165969 - 10/01/09 10:09 PM (8 years, 1 month ago)

Yeah. This says to be careful, but still, it's a risk. And why inject just 10 mL of air? Why not fill it up to even pressure?

But then why buy pre-made LC, right?


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InvisibleTacoHerder
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Re: LC question [Re: Doc_T]
    #11165978 - 10/01/09 10:11 PM (8 years, 1 month ago)

:shrug: Ask my gf that.  She also bought me 25 myco bags though... So that was good.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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Re: LC question [Re: TacoHerder]
    #11165990 - 10/01/09 10:13 PM (8 years, 1 month ago)

Can't argue w/ 25 mycobags. :thumbup:
What about this: go ahead and treat follow the directions, it just might work. Or you can see that it doesn't, and report back.


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InvisibleTacoHerder
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Re: LC question [Re: Doc_T]
    #11166012 - 10/01/09 10:19 PM (8 years, 1 month ago)

Ill just put 2 cc's worth of spore solution in the syringe ill use to inoculate the LC... Cant go wrong if i do that.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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Re: LC question [Re: TacoHerder]
    #11166073 - 10/01/09 10:34 PM (8 years, 1 month ago)

And 8 mL of sterile air. Then the process is automatic, it shoots itself for you! :thumbup:


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Re: LC question [Re: Doc_T]
    #11166128 - 10/01/09 10:45 PM (8 years, 1 month ago)

Ya, that would be good, 2 ml of spore solution, and the remaing 8ml  of sterile air.  That would take care of both issues at once:super:


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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Re: LC question [Re: TacoHerder]
    #11166225 - 10/01/09 10:59 PM (8 years, 1 month ago)

Thought experiment- instead of air, shoot in pure oxygen. Enough to bring it to even pressure or slight overpressure. Effect on myc will be... what?

Nothing, probably. But maybe something. I wonder how much vacuum is in an LC jar?


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Re: LC question [Re: Doc_T]
    #11166455 - 10/01/09 11:36 PM (8 years, 1 month ago)

:shrug:  i dunno where i  could findpure oxygen.  100% oxygen is pretty damn flamable, i wouldnt advise messing with it...


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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Re: LC question [Re: TacoHerder]
    #11166533 - 10/01/09 11:46 PM (8 years, 1 month ago)

Yeah, that's why it's a thought experiment. I could boost a bottle of O2, but I probably won't.


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Re: LC question [Re: Doc_T]
    #11166545 - 10/01/09 11:48 PM (8 years, 1 month ago)

your going to steal a bottle of o2 but dont like when ppl use tyvek from the PO, for things other than mailing shit?


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Re: LC question [Re: TacoHerder]
    #11166553 - 10/01/09 11:48 PM (8 years, 1 month ago)

Quote:

100% oxygen is pretty damn flamable




Not exactly, but it can be dangerous when around flammable substances.

Nitrogen scrubbers can be made with a filter and some sort of a compressor. Could get to 60-80% with home made materials, I would presume.

Short of that, I know there are places where you can get liquid O2, but I'll be damned if I know where or what kind of license you would need.


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Re: LC question [Re: truskool]
    #11166583 - 10/01/09 11:53 PM (8 years, 1 month ago)

Quote:

truskool said:
your going to steal a bottle of o2 but dont like when ppl use tyvek from the PO, for things other than mailing shit?




I said I could. I could steal a lot of things, but I don't. In the name of science I could justify a bottle if I ever got around to it- but like I said, probably won't happen.


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InvisibleSlimz
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Re: LC question [Re: Doc_T]
    #11166596 - 10/01/09 11:54 PM (8 years, 1 month ago)

its not stealing from the post office.. its a government agency.. I paid for it to start with


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Re: LC question [Re: Slimz]
    #11166605 - 10/01/09 11:55 PM (8 years, 1 month ago)

Wrong, PO isn't owned by the government, hasn't been since the 70's.


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Re: LC question [Re: Doc_T]
    #11166618 - 10/01/09 11:57 PM (8 years, 1 month ago)

I get tyvek from the post office.

If its free its free.

They dont exactly tell you that hey this is for mail purpose only do they?

Yes I know thats the purpose of it being there but if its free it just like grabbing a free newspaper.

So... IMO its not stealing


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Mycobags

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