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RonPaulVerm
Piss Christ


Registered: 07/21/08
Posts: 1,072
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Wet tissue transfer (agar)?
#11153338 - 09/29/09 08:52 PM (14 years, 4 months ago) |
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I had some mushroom tissue in distilled water in half pint for a couple days ( i wanna learn how to preserve longer) and then transferred to agar. The tissue was still slightly wet (not dripping) when transferred. Where it cam in contact with the agar is turning white. (i'm guessing contam)
When trandferring tissue should it be somewhat dry?
-------------------- Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech, Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have Primitive Skills Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms ...I DO advocate learning about Law Enforcement Against Prohibition
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solumvita
Q.B.E.


Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 8 months, 13 days
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Re: Wet tissue transfer (agar)? [Re: RonPaulVerm]
#11162377 - 10/01/09 07:09 AM (14 years, 4 months ago) |
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try get internal tissue if the piece is big enough. the white may be bacteria or it may be the start of mycelium.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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RonPaulVerm
Piss Christ


Registered: 07/21/08
Posts: 1,072
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Re: Wet tissue transfer (agar)? [Re: solumvita]
#11162524 - 10/01/09 08:16 AM (14 years, 4 months ago) |
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oh duh, internal tissue. well maybe you can answer my internal tissue question. I've transferred stringy internal tissue before and it just bruises and shrivels up a bit and doesn't appear to be thriving... any thoughts?
-------------------- Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech, Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have Primitive Skills Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms ...I DO advocate learning about Law Enforcement Against Prohibition
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solumvita
Q.B.E.


Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 8 months, 13 days
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Re: Wet tissue transfer (agar)? [Re: RonPaulVerm]
#11163530 - 10/01/09 12:11 PM (14 years, 4 months ago) |
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Not sure, if it has not grown within a week there is something wrong. What agar are you using, you could going to a softer agar so as to allow the tissue to sit on softer agar and enable the tissue to be hydrated from the agar.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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