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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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Offlinedoom876
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Registered: 08/01/08
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Last seen: 7 years, 8 months
sanitation
    #11160692 - 10/01/09 12:13 AM (8 years, 25 days ago)

Alright, I have tried on four jars, mountains of sanitizer, heated the syringe, covered face with shirt, used latex gloves, but they keep getting infected. They had the silicon port too. Any tips please?


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OfflineSillySiteMan
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Re: sanitation [Re: doom876]
    #11160712 - 10/01/09 12:15 AM (8 years, 25 days ago)

is the syringe contam?


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Offlineskullhuman
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Re: sanitation [Re: doom876]
    #11160722 - 10/01/09 12:17 AM (8 years, 25 days ago)

Do you use a glovebox? And were the jars all done from the same syringe?


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OfflineBrandNewbie
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Re: sanitation [Re: doom876]
    #11160768 - 10/01/09 12:23 AM (8 years, 25 days ago)

Most of the time I don't know what I'm talking about, just ask around.

BUT, you need to prepare more jars than you plan on using. You mentioned four jars. Let's say you prepped eight jars instead. After prepping eight jars, set one aside. The next step use the remaining seven jars. Then, again, set one aside. The next step use six jars, etc.

Each jar that you set aside will be a control jar. (you probably don't need eight, but you get the picture).
Now when you get a contaminate, the control jar that you set aside for that step should be contaminate free.
THIS way, you will know at what point the contaminate is being introduced. Once you know that, you will be able to take the appropriate measures to improve your sterilization technique for that step.

I know it sounds like a lot of extra work, but when you consider the work that you have already done that got ruined, it doesn't seem all that bad.

Hang in there and you WILL be successful! :goodluck:


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Offlinekingsmountainview
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Re: sanitation [Re: BrandNewbie]
    #11160822 - 10/01/09 12:32 AM (8 years, 25 days ago)

Yeah listen the the Capt.


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Offlinedoom876
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Re: sanitation [Re: kingsmountainview]
    #11160983 - 10/01/09 12:52 AM (8 years, 25 days ago)

Hm, that would cost oodles. How would someone without a glovebox. I'd imagine the contams are introduced when I screw the needle on. I was surprised it wasn't assembled, that sucked, because I poored hand sanitizer, Purel, all over the puncture zone, anmd the whole of one syringe went into into a jar.

I'd rather get a grow box, but those are tough to get here in Michigan.


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OfflineSillySiteMan
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Re: sanitation [Re: doom876]
    #11161022 - 10/01/09 12:57 AM (8 years, 25 days ago)

do it under a flow hood..thats all ive done and never had contam *knocks on wood*


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Offlineskullhuman
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Re: sanitation [Re: doom876]
    #11165938 - 10/01/09 10:03 PM (8 years, 24 days ago)

You can make a glove box pretty easily.  Why not give that a shot?


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InvisibleSlimz
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Re: sanitation [Re: skullhuman]
    #11166025 - 10/01/09 10:22 PM (8 years, 24 days ago)

please tell us the steps you are using.

try sterilizing the jars before adding the PF material
then make sure you boil for 90 min

what kind of filters do you use?


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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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