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Offlinewhypickthree
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Starting Over
    #11158503 - 09/30/09 07:04 PM (8 years, 8 months ago)

Well after 3 days my jars smell funky, which seems to indicate a bacterial contamination. I'll be throwing them out.

I'm probably going to start over, but need to know if I'll be able to harvest before the 23rd of November. I'll be leaving for a week that day and I don't have anyone who could look after them. I figure it would be a week for spores to get here and then a few days after that for sterilization and inoculation. So the 12th of October would be my target date for beginning of colonization. That would leave me with ~43 days to go from colonization to harvest. Can this be done or should I wait until after my trip?


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Invisiblefeelfunny
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Re: Starting Over [Re: whypickthree]
    #11158512 - 09/30/09 07:05 PM (8 years, 8 months ago)

yes you can get befor nov you could get by the end of OCT


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Offlinewhypickthree
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Re: Starting Over [Re: feelfunny]
    #11158520 - 09/30/09 07:07 PM (8 years, 8 months ago)

I assume that's with temperatures in the low to mid 70's though?


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Invisiblefeelfunny
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Re: Starting Over [Re: whypickthree]
    #11158531 - 09/30/09 07:08 PM (8 years, 8 months ago)

dosent have to be. i can get a jar colonized in 9-12 days


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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InvisibleCH HELL
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Re: Starting Over [Re: whypickthree]
    #11158542 - 09/30/09 07:10 PM (8 years, 8 months ago)

You should be able to get one flush by then,  but if colonization takes long then you can always put them in the fridge once they are 100% colonized.  This will delay the pinning and they will be ready to birth wen you get back from your vacation.
CH


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Offlinewhypickthree
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Re: Starting Over [Re: feelfunny]
    #11158634 - 09/30/09 07:25 PM (8 years, 8 months ago)

Thanks for the info. I'm trying to figure out where my sterile work fell flat, would you mind critiquing?

Here it is if you'd be so kind:

PC'd for 90 mins-2 hours: seems like it mostly boiled that whole time instead. double aluminum foil on each and a layer of verm on top.

Then I went into a pretty clean bathroom with the door shut to minimize air flow, that I had previously moped and wiped down with Clorox sheets. I used a container as a air flow "preventer" but instead of two arm holes I made one large hole in a side. I used a flame on the syringe for 15-20 seconds (water was popping and squirting out) and then wiped down the needle with 90% alcohol. I did this between each jar. I did one jar at a time and put the foil back on after each inoculation.

Off to school now, be back in 2.5 hours.


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Invisibleck5
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Re: Starting Over [Re: whypickthree]
    #11158660 - 09/30/09 07:28 PM (8 years, 8 months ago)

Maybe the syringe was contaminated.


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Invisiblefeelfunny
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Re: Starting Over [Re: whypickthree]
    #11158661 - 09/30/09 07:28 PM (8 years, 8 months ago)

sounds ok  but 2 hours is not nessisary and it is very posable you cooked your sub not starilize which is what you want


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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InvisibleCH HELL
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Re: Starting Over [Re: feelfunny]
    #11158716 - 09/30/09 07:35 PM (8 years, 8 months ago)

Don't wipe the needle after flaming it,  there is no need just give it a little squirt to cool it off.
Keep the foil off after inoculating the dry verm is you contam barrier.
CH


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Amazon Shop: ½ Pint Jars, Vermiculite

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