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Offlinedog man
biologicallyengineered

Registered: 12/05/02
Posts: 28
Last seen: 13 years, 11 months
a couple newbie questions
    #1115093 - 12/05/02 08:52 PM (14 years, 1 day ago)

I innoculated 12 pf tek jars 4 days ago. About 5 of the jars are showing bright white mycelium at the innoculation points, 4 are showing a lighter white at innoculation points, and the rest are showing no growth at all. First, when mycelium first appears, is it "light" white or is this cobwebs? Second, I've had trouble stabelizing the temperature of my incubaltion chamber. Because it obviously heats up during the day, the temps get up to about 87 degrees and then drop to about 82 at night. I'm gone all day so theres really not much I can do about this. Could this excess heat have dried out the jars which are not showing growth? If so, should I inject some sterylized water into these jars? I know, I know; I'm probably being impatient, but I wanted to run this past some people w/ experience just to make sure. Thanks


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OfflineRabbitt
Fucktard=k3y
 User Gallery

Registered: 11/12/02
Posts: 1,498
Last seen: 9 years, 7 months
Re: a couple newbie questions [Re: dog man]
    #1115118 - 12/05/02 08:56 PM (14 years, 1 day ago)

Your temps are perfect even if u cant stabilize it thats a very good range for incubation
Just be patient and you will reap the benefits when the time is right


--------------------
[NubS]


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Invisibledaussaulit
Forgetful

Registered: 08/06/02
Posts: 2,894
Loc: Earth
Re: a couple newbie questions [Re: dog man]
    #1115120 - 12/05/02 08:57 PM (14 years, 1 day ago)

>First, when mycelium first appears, is it "light" white or is this cobwebs?

will need a picture to determine.

>Because it obviously heats up during the day, the temps get up to about 87 degrees and then drop to about 82 at night. I'm gone all day so theres really not much I can do about this. Could this excess heat have dried out the jars which are not showing growth?

Maybe. 87 is a little higher than what it needs but shouldn't make a detrimental difference.

>If so, should I inject some sterylized water into these jars?
Sterilized. No, you'll increase your chance of contams greatly.

>I'm probably being impatient
yes you are, leave the jars alone and don't look at them for at least 3 days.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 13 years, 7 months
Re: a couple newbie questions [Re: daussaulit]
    #1115235 - 12/05/02 09:25 PM (14 years, 1 day ago)

>87 is a little higher than what it needs but shouldn't make a detrimental difference.

Not at all. As long as it wasn't exposed to this temp. for an extended period of time. I believe I've heard that 96 degrees is the official temperature that cooks the mycelium.


--------------------
The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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Invisibledaussaulit
Forgetful

Registered: 08/06/02
Posts: 2,894
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Re: a couple newbie questions [Re: Angry Mycologist]
    #1115279 - 12/05/02 09:34 PM (14 years, 1 day ago)

You mean 106.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 13 years, 7 months
Re: a couple newbie questions [Re: daussaulit]
    #1115386 - 12/05/02 10:13 PM (14 years, 1 day ago)

Errr, yes 106.


--------------------
The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 11 years, 7 months
Re: a couple newbie questions [Re: dog man]
    #1115460 - 12/05/02 10:40 PM (14 years, 1 day ago)

In reply to:

First, when mycelium first appears, is it "light" white or is this cobwebs?


Sometimes mycelium starts out greyish and turns a vigorous white as the mycelium grows. In other words, you will be able to tell for sure in a couple of days.


--------------------
Re-Defeat Bush in '04


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