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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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20 year old mushroom ground up with spores in it
    #11145339 -

it was picked wild by me 20 years ago dehydrated and put in a capsule.
it was stored under the worst conditions possable from hot to frozen cold.

if i hydrate it and plate it will it grow or is there something else i need to do?

i have micro slides etc from then when i picked it and an lc tek "new tek which was just starting out"  i printed off the net from a sight like this one. wish i knew the name of the sight from back then. well not exactly printed but notes taken from the net and mag's i was reading at the time.

i would like to pick up were i left off and try to get it to germinate.

some of the spores under the scope seem to have germinated with the squiggly things "name escapes me right now" coming out the germ pore

a high number oh 50% have no germination.

i was wondering at what rate do the spores die or what is the half life under the worst condition of spores.

this sight has helped me so much now and this is a big deal for me. i hope one and all can give some good suggestions.


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some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11145353 -

i doubt any of them are even still viable..

if you need a print shoot me a PM..


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:shrug:

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Re: 20 year old mushroom ground up with spores in it [Re: nastos]
    #11145360 -

You should still try growing them, it can't hurt

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Re: 20 year old mushroom ground up with spores in it [Re: nastos]
    #11145378 -

i have heard of much crazier things working. i would take a small amount hydrate it and place it on some agar. if you have antibiotic media use it.

whats the worst that can happen? it doesnt germinate, oh well. i vote try it, clean it and grow it.


--------------------
Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

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Re: 20 year old mushroom ground up with spores in it [Re: HambuHodo]
    #11145381 -

HambuHodo said:
You should still try growing them, it can't hurt



i want to but i need to know the most optimal way to go about it.

and thanks nast...  i will pm you later but i want to focus on this project right now.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: LitCloset]
    #11145427 -

LitCloset said:
i have heard of much crazier things working. i would take a small amount hydrate it and place it on some agar. if you have antibiotic media use it.

whats the worst that can happen? it doesn't germinate, oh well. i vote try it, clean it and grow it.



cool what i was thinking but i have no antibiotic but will check around

what type is best and how much do i add to my 500ml batches.

also what is the best temp to incubate at i have an incubator that is very precise and it does humidity too " egg type real big converted for my other projects"

also it is not that much so should i put a pin head amount into hydration or more.

the pin head i just put under the scope looked like 1000's of spores.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11145438 -

deffinatley keep us posted. sounds like a col project at least.


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:shrug:

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Re: 20 year old mushroom ground up with spores in it [Re: nastos]
    #11145465 -

i heard some stuff about snake venom? maybe this will work. i have no idea just throwing it out there

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Re: 20 year old mushroom ground up with spores in it [Re: nastos]
    #11145471 -

thanks ...tos

will try but you know get caught up in it an forget pics etc.

Quote:
i heard some stuff about snake venom? maybe this will work. i have no idea just throwing it out there


what?

were the hell would i get that man.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11145524 -

You wont be able to get snake venom and its used for something else. You can buy antibio agar easy, but it will be hard to get the antibio on its own, i think they use gentamicin Im not 100%.

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Re: 20 year old mushroom ground up with spores in it [Re: badman]
    #11145544 -

you should be able to ger gentamicin at a feed store


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Feel Family Right Hand

Join us in Shroomery text chat! #feelfamily

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Re: 20 year old mushroom ground up with spores in it [Re: badman]
    #11145555 -

badman said:
You wont be able to get snake venom and its used for something else. You can buy antibio agar easy, but it will be hard to get the antibio on its own, i think they use gentamicin Im not 100%.


thanks man im not so good with snakes don't you know. but i do have a good doc. so gent is the kine and what is the dose for 500ml of ag.

Quote:
you should be able to ger gentamicin at a feed store


good point too no med insurance needed there .


but the dose guys


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11145585 -

Oh god id use 0.1-0.2 g per 1000 ml but ive always used premade antibio agar mix.

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Re: 20 year old mushroom ground up with spores in it [Re: badman]
    #11145835 -

i would take a small amount about the size of say 2mm^2. place that in a 1-3ml pcr tube and add minimal mineral salts solution, about .5ml. vortex/mix well and allow to sit overnight.

then taking a 10uL steriloop do an isolation streak on pda or similar agar. repeat on as many plate as you deem neceassary. if growth is not seen after 1 month try again with a more concentrated solution

any and all mycelium growth that is free from major contamination should be transfered to plate of pda or similar media with hydrogen peroxide. from there just treat it like anyother muchroom culture


--------------------
Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: LitCloset]
    #11146905 -

LitCloset said:
i would take a small amount about the size of say 2mm^2. place that in a 1-3ml pcr tube and add minimal mineral salts solution, about .5ml. vortex/mix well and allow to sit overnight.

then taking a 10uL steriloop do an isolation streak on pda or similar agar. repeat on as many plate as you deem neceassary. if growth is not seen after 1 month try again with a more concentrated solution

any and all mycelium growth that is free from major contamination should be transfered to plate of pda or similar media with hydrogen peroxide. from there just treat it like anyother muchroom culture



sounds like the plan thanks

what is the tek called
by the way
if it has a name


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11146977 -

LOL...we'll call it the "vintage" keep us posted on your results


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Toxic/Hallucinogenic spores are for microscopy purposes, all posts regarding their cultivation fall under one of three categories: 1) hypothetical scenario 2) complete bullshit 3) complete hypothetical bullshit. Any questions?

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Re: 20 year old mushroom ground up with spores in it [Re: kingsmountainview]
    #11146995 -

sorry no tek except what i wrote, off the top o me head


--------------------
Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: LitCloset]
    #11147228 -

LitCloset said:
sorry no tek except what i wrote, off the top o me head


cool i was thinking along those lines but i just wanted to do a search to see it drawn out. my bad

good head man off the top.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Re: 20 year old mushroom ground up with spores in it [Re: ratdog]
    #11147385 -

ratdog said:
thanks ...tos

will try but you know get caught up in it an forget pics etc.

Quote:
i heard some stuff about snake venom? maybe this will work. i have no idea just throwing it out there


what?

were the hell would i get that man.



RR used it to make PE #6 as the story goes


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Re: 20 year old mushroom ground up with spores in it [Re: feelfunny]
    #11147537 -

so to cross stuff you need snakes but to germinate 20 year old spores all i need is some salt water???????????


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 |  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   MagicBag.co All-In-One Bags That Don't Suck

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