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Offlinenatty
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Registered: 11/21/02
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Last seen: 13 years, 3 months
h202 bath???
    #1113968 - 12/05/02 03:42 PM (14 years, 7 months ago)

Hi all,
AFOAF just completed his first successful PFtek style batch and wants to clone a new batch. He has read up on cloning and is planning on making some honey water to innoculate with. The question is this:
If he takes a shroom from the first batch and gives it a dunk in h202, will that work to kill surface contams and will the shroom tissue survive the process to innoculate the honey water? He would love to hear from anyone who has done a similar thing, but speculation would be nice too. Thanks

Natty





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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: natty]
    #1114513 - 12/05/02 05:50 PM (14 years, 7 months ago)



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Offlinenatty
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Registered: 11/21/02
Posts: 3
Last seen: 13 years, 3 months
Re: h202 bath??? [Re: vatoloco]
    #1114574 - 12/05/02 06:13 PM (14 years, 7 months ago)

Hi,
Thanks for the reply. Let me elaborate on the idea my friend had. Take the shroom, give it a dunk in the H2O2, then chop it up in a sterilized blender with some sterilized water. Then use this mixture to innoculate the honey water. His thinking was that the H2O2 would kill surface contams, and even if it killed the outer layer of the shroom, the clean inner layer would still be blended. Any opinions on this would be welcome.

Natty


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: natty]
    #1114580 - 12/05/02 06:16 PM (14 years, 7 months ago)



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InvisibleSixCee
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Registered: 06/12/02
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Re: h202 bath??? [Re: vatoloco]
    #1114711 - 12/05/02 07:00 PM (14 years, 7 months ago)

You cant get pure h202. What is the concentration? The most common is 3%


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Offlinenatty
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Registered: 11/21/02
Posts: 3
Last seen: 13 years, 3 months
Re: h202 bath??? [Re: SixCee]
    #1115209 - 12/05/02 09:17 PM (14 years, 7 months ago)

Ya, He would be using 3%


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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