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InvisibleCordyceps
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Hydrogen peroxide
    #11137801 - 09/27/09 05:13 PM (11 years, 7 months ago)

Hi all,

I saw some people use Hydrogen peroxide to decrease contamination.

Please can anybody tell me how they use this can I add this to my agar ?? so yes how many % must I have and what is the dosage ??

Can I also use Hydrogen peroxide for my grain substrate ?? hopefully somebody can tell me more about using Hydrogen peroxide for mushrooms

I'm new here.


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InvisibleDoc_T
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Re: Hydrogen peroxide [Re: Cordyceps]
    #11137820 - 09/27/09 05:16 PM (11 years, 7 months ago)

No need for peroxide. Use it to combat cobweb mold, if and when that's an issue. Otherwise, not useful.


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Re: Hydrogen peroxide [Re: Cordyceps]
    #11137839 - 09/27/09 05:21 PM (11 years, 7 months ago)

H2O2 used to be thought of as a super contaminate killer. Not anymore.

It's used today in your standing water, like a cool mist, to help keep the water from stagnating as much.

It's also used to kill Dactylium, (or cobweb) which it works well for. Because Trich is more prevalent on agar plates it might be an idea to use it in agar, but I don't know enough to comment on that.
:thumbup:

Edited: corrected mold.



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Edited by BrandNewbie (09/27/09 05:28 PM)


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Re: Hydrogen peroxide [Re: BrandNewbie]
    #11137850 - 09/27/09 05:24 PM (11 years, 7 months ago)

I've hard of people using brown cardboard instead of agar.


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InvisibleDoc_T
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Re: Hydrogen peroxide [Re: BrandNewbie]
    #11138097 - 09/27/09 06:07 PM (11 years, 7 months ago)

Quote:

BrandNewbie said:
It's used today in your standing water, like a cool mist, to help keep the water from stagnating as much.





Rinsing out the chamber once in a while is plenty, IMO. But peroxide is certainly harmless in a humidifier.


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OfflineRogerRabbitM
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Re: Hydrogen peroxide [Re: Doc_T]
    #11138832 - 09/27/09 08:19 PM (11 years, 7 months ago)

I don't use it on agar.  Since the purpose of petri dishes are to isolate healthy strains from each other or from contaminant molds and/or bacteria, let the molds readily grow so you can see them.  You then transfer healthy mycelium away from the contaminated dish and to new ones.  Repeat until you have a pure culture.

Peroxide is great for cobweb on a casing layer though.  In that case use it at 3%, saturating the casing layer, and repeating twice again at 12 hour intervals.
RR


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