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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Problems getting Rhizo growth from MEA [Re: On_the_Down-Low]
    #1095640 -

7days is a little to short time to see rhizo?s...
Patience...!!!

peace...

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Re: Problems getting Rhizo growth from MEA [Re: Roadkill]
    #1095658 -

Those amounts are to pour how many dishes?


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Support bacteria - they're the only culture some people have.

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1095865 -

Depends on how much you pour in each petri..and the size of it.
For standard size approx. ~25ml to each disk..

peace...

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Re: Problems getting Rhizo growth from MEA [Re: stonErollEr1]
    #1098113 -

Checked out some instant spuds the other day...Idahoan....they had a bunch of other stuff in them...preservatives and what not.....

Are those cool or do the contents need to be just 100% spuds...?


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Support bacteria - they're the only culture some people have.

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1100376 -

They?re probably allright..

Peace...

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1101483 -

Try to go organic if possible. Where did/would you get BRF?

What if the armadillo took a nice clump of rhizomes from a a jar of grain,and then grew it out on agar?
If he didn't do it from grain, just from other agar, how would he chose the speciman for the 2nd stage grow out on agar?

Petris seem a lot better then 1/2pints at this point.

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Re: Problems getting Rhizo growth from MEA [Re: On_the_Down-Low]
    #1103772 -

Petris is the way to go..?pints is way to clumpsy to work with.
Specimen? Do you plan on fruiting straight from the agar and then clone the first shroom that appears? or do you mean, what part of the mycelium to choose?
If the later..take the healthiest looking part..or if you spot rhizo?s..its just selective growing..so goahead and select what part you want..[it all depends on what charisteristics[sp?] you are looking for.]
And then isloate it so you get an "isloated" pure culture..[without sectoring]

peace...

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Re: Problems getting Rhizo growth from MEA [Re: stonErollEr1]
    #1104194 -

Is there any difference if the first mycelium comes from grain or from agar,when you do the second transfer to agar?

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Re: Problems getting Rhizo growth from MEA [Re: On_the_Down-Low]
    #1105778 -

The source of the mycelium shouldn't matter in this case, but I would suggest using peroxidated agar to help keep your mycelium uncontaminated. and oh so pearly white. *ha ha*
Actually if you get some nice rhizomorphic growth from the grain that might even give your selection process a head start.


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Re: Problems getting Rhizo growth from MEA [Re: Alkaloids]
    #1107612 -

I wondered about that and thought someone asked that question already somewhere but I have been doing way too much research....

How bout it? Is that Kosher? Taking rhyzo growth out of a grain jar and transfering it to the agar and going off of that?


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Support bacteria - they're the only culture some people have.

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1110312 -

I hope so. The dillo said some of the rye grain jars were very rhizomorphic,but the agar is no where near as nice looking. So what's the advantage of agar again? Was the difference due to that strains preference forrye?or some other reason?

Edited by On_the_Down-Low (12/04/02 09:35 PM)

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Re: Problems getting Rhizo growth from MEA [Re: On_the_Down-Low]
    #1111158 -

Supposedly because you get a strain that is ryhzomorphic thus more prone to fruiting as ryhzomorphic growth is related to pinning, but is also seems that you have to test the new strain out becuase it may not have the same charecteristics as the original parents ie the shroom you took the print from....if i got that right....


--------------------
Support bacteria - they're the only culture some people have.

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1112819 -

Exactly...since there is somewhat diffent geneticts due to the multispore innoc..
A clone has the same genetics as the cloned mushroom though.

peace...

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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