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OfflineSillyBilly
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114038 - 09/23/09 05:15 PM (14 years, 4 months ago)

bingo just watched RR's agar section again
boil over is a very popular problem
i must have no used enough water, thought i was pretty generous


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    You're a better man than I am, Gunga Din


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OfflineRogerRabbitM
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114066 - 09/23/09 05:20 PM (14 years, 4 months ago)

The agar boils over as the PC cools off.  The extra water slows down the cooling.  You want the pressure cooker to lose steam pressure very slowly.  If necessary, turn the stove back on a few times as the pressure drops to slow down the process.  This is usually much more of a problem with made in china pressure cookers that are very thin.  The thicker AA models hold heat better.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineSillyBilly
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Re: Erlenmeyer flask pissing me off [Re: RogerRabbit]
    #11114143 - 09/23/09 05:28 PM (14 years, 4 months ago)

alright cool thank you


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By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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Invisiblefeelfunny
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114209 - 09/23/09 05:35 PM (14 years, 4 months ago)

good luck let us know if it dose better


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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OfflineSillyBilly
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Re: Erlenmeyer flask pissing me off [Re: feelfunny]
    #11114279 - 09/23/09 05:44 PM (14 years, 4 months ago)

lol i dont know whats going to be harder, isolating a strain of pan cyan or just making the petri dishes...


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    You're a better man than I am, Gunga Din


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Invisiblefeelfunny
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114392 - 09/23/09 05:59 PM (14 years, 4 months ago)

i bet you thought you new the ancwer befor you got started with the dishes:grin:


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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OfflineSillyBilly
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Re: Erlenmeyer flask pissing me off [Re: feelfunny]
    #11114414 - 09/23/09 06:02 PM (14 years, 4 months ago)

before i get to pouring them
i have a question, regarding the solidfication
when pouring them, i assume you stack them and let them solidify in front of the flowhood without putting parafilm on them if you plan to use them that day?
or should you cover them with parafilm regardless while letting them solidify?


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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Invisiblefeelfunny
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114549 - 09/23/09 06:23 PM (14 years, 4 months ago)

i let mine sit in the glove box till cool and use them then seal not sure about a flow hood


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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OfflineSillyBilly
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Registered: 03/05/09
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Re: Erlenmeyer flask pissing me off [Re: feelfunny]
    #11114597 - 09/23/09 06:29 PM (14 years, 4 months ago)

alright, probably works best anyway because of condensation


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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InvisibleCH HELL
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11114617 - 09/23/09 06:32 PM (14 years, 4 months ago)

Quote:

SillyBilly said: stack them and let them solidify in front of the flowhood without putting parafilm on them.


:thumbup:


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Invisibleratdog
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Re: Erlenmeyer flask pissing me off [Re: CH HELL]
    #11117646 - 09/24/09 06:04 AM (14 years, 4 months ago)

i make beer and use malt ext,  i have to boil it slow till th proteins drop out and boil over stops. so try to boil it first then pc.


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some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:


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OfflineRogerRabbitM
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Re: Erlenmeyer flask pissing me off [Re: SillyBilly]
    #11118019 - 09/24/09 08:52 AM (14 years, 4 months ago)

Quote:

SillyBilly said:
before i get to pouring them
i have a question, regarding the solidfication
when pouring them, i assume you stack them and let them solidify in front of the flowhood without putting parafilm on them if you plan to use them that day?
or should you cover them with parafilm regardless while letting them solidify?




Slip the plastic sleeve the dishes came in over the top of the stack as they cool and solidify.  If you try to wrap the still liquid agar dishes with parafilm, you'll spill the agar out.  I leave the dishes in the plastic sleeve for several days sometimes until I use them.  I don't wrap with parafilm until after the dishes have been inoculated.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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