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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
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Last seen: 13 years, 11 months
Sterile Shrooms
    #1111432 - 12/04/02 10:06 PM (14 years, 9 days ago)

I just harvested my second casing of sterile shrooms in a row. There are no spores at all. The strain is B+ grown on cased birdseed and they are all absolutely sterile (and yes they were very mature). I guess I was just wondering if there is anything that could cause this other than just a genetic defect. I'm getting really low on spores and I really need to get some more but I can't seem to produce any.
Thanks in advance


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"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


Edited by New Name (12/04/02 10:36 PM)


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OfflineStS
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Re: Sterile Shrooms [Re: New Name]
    #1111441 - 12/04/02 10:08 PM (14 years, 9 days ago)

Describe your spore printing method, including when you take the cap and where you place it to print.


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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
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Re: Sterile Shrooms [Re: StS]
    #1111466 - 12/04/02 10:12 PM (14 years, 9 days ago)

I take the cap off when the edges of the cap start to turn upwards or when the caps start dropping spores everywhere. Then I place it on some sterile wax paper and put a jar over it for usually about 24 hours. I have done this many times in the past with no problems but my last few batches of shrooms haven't produced any spores at all.


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--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
Posts: 230
Loc: ATL
Last seen: 13 years, 11 months
Re: Sterile Shrooms [Re: New Name]
    #1112390 - 12/05/02 02:59 AM (14 years, 9 days ago)

hate to do it, but *bump*


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--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


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OfflineExtravagantDream
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Re: Sterile Shrooms [Re: New Name]
    #1112470 - 12/05/02 03:53 AM (14 years, 9 days ago)

umm ummm ummm... hmmmm

maybe ur not waiting long enough.. sometimes it takes a little longer then 24 hours. Maybe, you have been taking spores from genetically weak sporulating shrooms, and have therefor increase the chance of sterile shrooms. I really dont freakin know. I wish I could help but I really dont know why this is occuring. Psilocybe Cubensis usually readily sporulate w/o any type of special conditions.


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OfflineDERRAYLD
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Registered: 05/13/02
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Re: Sterile Shrooms [Re: ExtravagantDream]
    #1112478 - 12/05/02 03:59 AM (14 years, 9 days ago)

You stated that you take the caps to print when they begin to drop spores????
So if they`re dropping spores then why exactly are they sterile??


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OfflineExtravagantDream
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Re: Sterile Shrooms [Re: DERRAYLD]
    #1112483 - 12/05/02 04:01 AM (14 years, 9 days ago)

I think that has been in his previous attempts, but recently they have been sterile. Thats how i understand it.

ohh and BTW i stated no such thing.


Edited by ExtravagantDream (12/05/02 04:02 AM)


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OfflineDERRAYLD
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Re: Sterile Shrooms [Re: ExtravagantDream]
    #1112484 - 12/05/02 04:03 AM (14 years, 9 days ago)

That`s how I understood it as well :wink:
Why not stir the pot to massage his nueral impulse to his brain?
Make him think a bit for himself :smile:

An idea would be to clone a piece of a mushroom in some peroxidated Honey water and then use the resulting spore solution.
Or transfer a pice to agar and create a viable isolate from that?


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Derrayld and out of control


Edited by DERRAYLD (12/05/02 04:04 AM)


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OfflineExtravagantDream
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Re: Sterile Shrooms [Re: DERRAYLD]
    #1112487 - 12/05/02 04:04 AM (14 years, 9 days ago)

I'm not sure if there are enough neuro-transmitters left. I was just trying to get a question answered without being a smart ass for once.

If you clone a sterile shrooms, I'm pretty sure its gonna be sterile. But you would be able to continue growth for some time that way.

*edit* I cant freakin spell


Edited by ExtravagantDream (12/05/02 04:08 AM)


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OfflineStS
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Re: Sterile Shrooms [Re: New Name]
    #1112634 - 12/05/02 05:06 AM (14 years, 9 days ago)

Well your method sounds ok. If you have successfully taken prints before, then I'm guessing you have a mutated strain. Maybe just try picking a little earlier (or later) and letting it print longer. Other than that, I don't know what to tell you.


--------------------

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OfflineNew Name
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Re: Sterile Shrooms [Re: New Name]
    #1112975 - 12/05/02 10:29 AM (14 years, 9 days ago)

Thanks for the help. I guess I will try and start anew with an older print and see if that fixes the problem.


--------------------
--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


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OfflineDERRAYLD
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Re: Sterile Shrooms [Re: New Name]
    #1112985 - 12/05/02 10:39 AM (14 years, 9 days ago)

Give your 2nd flush a chance to redeem itself.


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Derrayld and out of control


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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
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Last seen: 13 years, 11 months
Re: Sterile Shrooms [Re: DERRAYLD]
    #1112993 - 12/05/02 10:42 AM (14 years, 9 days ago)

Second flush is coming in right now. Maybe they will produce a few spores.


--------------------
--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
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Re: Sterile Shrooms [Re: New Name]
    #1113012 - 12/05/02 10:50 AM (14 years, 9 days ago)

That`s my point, yes.


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Derrayld and out of control


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InvisibleAlkaloids
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Re: Sterile Shrooms [Re: New Name]
    #1114680 - 12/05/02 06:47 PM (14 years, 9 days ago)

were these from a multi-spore inoculation? If so you may just have a sterile sub-strain. Happens every once in a while.


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
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Re: Sterile Shrooms [Re: Alkaloids]
    #1115871 - 12/06/02 01:12 AM (14 years, 8 days ago)

If it's from a multi-spore inoculation then there will be many different substrains, and the likelihood of all of them being sterile is slim. Something wrong with the growing conditions is my guess, not enough light maybe?
I had probs getting good sporeprints when I was still growing "closet style".


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hmmm........


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