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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 13 years, 4 months
Opinions on complete failure to colonize
    #1111205 - 12/04/02 09:21 PM (14 years, 2 months ago)

I innoculated a few jars with pink buffalo a couple weeks ago, and one simply hasn't shown any signs of colonization at all. The sites where they were innoculated only became dark and moist in appearance, and no mycelium appear present at all. Anyone else experience this? Just kind of curious why this may be.


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Offlinehappygrins
addict

Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 12 years, 15 days
Re: Opinions on complete failure to colonize [Re: TheHobbit]
    #1111218 - 12/04/02 09:25 PM (14 years, 2 months ago)


Did you remeber to shake the syringe before each innoculation? 

--happygrins :grin:


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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 13 years, 4 months
Re: Opinions on complete failure to colonize [Re: happygrins]
    #1111224 - 12/04/02 09:27 PM (14 years, 2 months ago)

The others came along ok, and I shake between innoculations, so I'm really at a loss - you'd think that you'd see something, but they're just damp spots....kinda weird.


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Offlineajg2425
farmer

Registered: 11/25/02
Posts: 202
Last seen: 13 years, 11 months
Re: Opinions on complete failure to colonize [Re: TheHobbit]
    #1111370 - 12/04/02 09:55 PM (14 years, 2 months ago)

was the jar cooled sufficiently after sterilization? :shocked:


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 13 years, 9 months
Re: Opinions on complete failure to colonize [Re: TheHobbit]
    #1111572 - 12/04/02 10:48 PM (14 years, 2 months ago)

Elaborate a bit more on your methods. Sterilization, Substrate, Spore Vender, Sexual Preference, etc.


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineDr_Gonzo
Is this not asuitable placeto park?

Registered: 10/31/02
Posts: 10
Last seen: 12 years, 11 months
Re: Opinions on complete failure to colonize [Re: TheHobbit]
    #1111587 - 12/04/02 10:54 PM (14 years, 2 months ago)

How about smells? Any funk coming out of the jars?

Dr. G.


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Offlinejoeshitragpicker
Home Sweet Home

Registered: 10/17/02
Posts: 1,265
Loc: Atlanta
Last seen: 3 years, 4 months
Re: Opinions on complete failure to colonize [Re: Dr_Gonzo]
    #1111714 - 12/04/02 11:38 PM (14 years, 2 months ago)

my gf threw out 3 jars that showed absoulutely nothing and they had been sitting there for 2 1/2 weeks.. eventually the wet spot goes away and then theres nothin..


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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 13 years, 4 months
Re: Opinions on complete failure to colonize [Re: Angry Mycologist]
    #1112769 - 12/05/02 06:45 AM (14 years, 2 months ago)

Ok, wow, even though it's been probably three wekks or more since innoculation, and there was still nothing there yesterday, the jar is now starting to colonize - it must have been waiting for me to post in regards to it. It's a basic verm/brf 1/2 pinter.....i think i may not have tamped down sufficiently, and maybe that's slowed up colonization so. They're from Hawkseye, and his syringes seem to have more than adequate spores floating around in 'em. Just weird, though, as the sites just sat there as damp looking spots....i only didn't toss it by now just out of curiosity, but lloks like it might make it after all.  :smile:


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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