|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Agent MadHatter
Mad as a Hatter


Registered: 07/23/09
Posts: 944
Last seen: 10 years, 5 months
|
What would one need to grow Claviceps Paspali?
#11108166 - 09/22/09 06:31 PM (14 years, 5 months ago) |
|
|
Its all in the title, but what would I need to get in order to grow Claviceps Paspali? And NOT get poisoned.
-------------------- "May the long-time sun shine upon you And all love surround you And the clear light within you Guide your way home." "Its never too late to start beefing up your obituary"- The Most Interesting Man in the World
|
Mykologist
Often too lazy to post...



Registered: 10/06/08
Posts: 1,586
Loc: I'm still here...
Last seen: 4 days, 19 hours
|
Re: What would one need to grow Claviceps Paspali? [Re: Agent MadHatter]
#11108197 - 09/22/09 06:35 PM (14 years, 5 months ago) |
|
|
I'm particularly interested in this as well...I'd assume you need to grow rye in a very humid, stale environment. Make sure you keep growing new rye in the greenhouse and extract alkaloids from the harvested rye?
I wanna know as well... Myk
--------------------
  Where would you rather be?
|
Agent MadHatter
Mad as a Hatter


Registered: 07/23/09
Posts: 944
Last seen: 10 years, 5 months
|
Re: What would one need to grow Claviceps Paspali? [Re: Mykologist]
#11108251 - 09/22/09 06:44 PM (14 years, 5 months ago) |
|
|
I have no clue, never grown shrooms before. But yeah, and extraction is the goal.
-------------------- "May the long-time sun shine upon you And all love surround you And the clear light within you Guide your way home." "Its never too late to start beefing up your obituary"- The Most Interesting Man in the World
|
SillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 2 months
|
Re: What would one need to grow Claviceps Paspali? [Re: Agent MadHatter]
#11108283 - 09/22/09 06:49 PM (14 years, 5 months ago) |
|
|
this has bad idea written all over it
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
|
Agent MadHatter
Mad as a Hatter


Registered: 07/23/09
Posts: 944
Last seen: 10 years, 5 months
|
Re: What would one need to grow Claviceps Paspali? [Re: SillyBilly]
#11109082 - 09/22/09 08:58 PM (14 years, 5 months ago) |
|
|
Why?
-------------------- "May the long-time sun shine upon you And all love surround you And the clear light within you Guide your way home." "Its never too late to start beefing up your obituary"- The Most Interesting Man in the World
|
SillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 2 months
|
Re: What would one need to grow Claviceps Paspali? [Re: Agent MadHatter]
#11109179 - 09/22/09 09:15 PM (14 years, 5 months ago) |
|
|
because its very dangerous and the only people who should mess with it have already a very good understanding of what theyre dealing with im pretty sure being around it isnt going to necessarily poison you but its not a good idea just in case it somehow ends up getting in your system, plus it could end up getting all over your grow area for future mushrooms and then you have a big problem with consumption if you're trying to make lsd, the phrase if you have to ask you'll never know comes to mind you need a very good understanding of chemistry before you should even attempt it until you know what you are doing, for your safety i really recommend not involving yourself with it
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
|
Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
|
Re: What would one need to grow Claviceps Paspali? [Re: Agent MadHatter]
#11109193 - 09/22/09 09:17 PM (14 years, 5 months ago) |
|
|
I very seriously doubt that you would succeed but if you try please keep us informed...
These were found at erowid... extremely help site. I will also link to a thread here at the shroomery at the bottom.
The Culture and Extraction of Ergot Alkaloids
Make up a culture medium by combining the following ingredients in about 500 milliliters of distilled water in a 2 liter, small-neck flask:
Sucrose .......................................... 100 grams Chick pea meal .................................... 50 grams Calcium nitrate ..................................... 1 gram Monopotassium phosphate ......................... 0.25 grams Magnesium sulphate .............................. 0.25 grams Potassium chloride ............................. 0.125 grams Ferrous sulphate heptahydrate ................... 8.34 milligrams Zinc sulphate heptahydrate ...................... 3.44 milligrams
Add water to make up one liter, adjust pH 4 with ammonia solution and citric acid. Sterile by autoclaving.
Inoculate the sterilized medium with Claviceps purpurea under sterile conditions, stopper with sterilized cotton and incubate for two weeks periodically testing and maintaining pH 4. After two weeks a surface culture will be seen on the medium. Large-scale production of the fungus can now begin.
Obtain several ordinary 1 gallon jugs. Place a two-hole stopper in the necks of the jugs. Fit a short (6 inch) glass tube in one hole, leaving 2 inches above the stopper. Fit a short rubber tube to this. Fill a small (500 milliliter) Erlenmeyer flask with a dilute solution of sodium hypochlorite, and extend a glass tube from the rubber tube so the end is immersed in the hypochlorite. Fit a long, glass tube in the other stopper hole. It must reach near the bottom of the jug and have about two inches showing above the stopper. Attach a rubber tube to the glass tube as short or as long as desired, and fit a short glass tube to the end of the rubber tube. Fill a large, glass tube (1 inch x 6 inches) with sterile cotton and fit 1-hole stoppers in the ends. Fit the small, glass tube in end of the rubber tube into 1 stopper of the large tube. Fit another small glass tube in the other stopper. A rubber tube is connected to this and attached to a small air pump obtained from a tropical fish supply store. You now have a set-up for pumping air from the pump, through the cotton filter, down the long glass tube in the jug, through the solution to the air space in the top of the jug, through the short glass tube, down to the bottom of the Erlenmeyer flask and up through the sodium hypochlorite solution into the atmosphere. With this aeration equipment you can assure a supply of clean air to the Claviceps purpurea fungus while maintaining a sterile atmosphere inside the solution.
Dismantle the aerators. Place all the glass tubes, rubber tubes, stoppers and cotton in a paper bag, seal tight with wire staples and sterilize in an autoclave.
Fill the 1-gallon jugs 2/3 to 3/4 full with the culture medium and autoclave.
While these things are being sterilized, homogenize in a blender the culture already obtained and use it to inoculate the media in the gallon jugs. The blender must be sterile. Everything must be sterile.
Assemble the aerators. Start the pumps. A slow bubbling in each jug will provide enough oxygen to the cultures. A single pump can, of course, be connected to several filters.
Let everything sit a room temperature (25 C) in a fairly dark place (never expose ergot alkaloids to bright light - they decompose) for a period of ten days.
After ten days adjust the culture to 1% ethanol using 95% ethanol under sterile conditions. Maintain growth for another two weeks.
After total of 24 days growth period the culture should be considered mature. Make the culture acidic with tartaric acid and homogenize in a blender for one hour.
Adjust to pH 9 with ammonium hydroxide and extract with benzene or chloroform/iso-butanol mixture.
Extract again with alcoholic tartaric acid and evaporate in a vacuum to dryness. The dry material in the salt (i.e., the tartaric acid salt, the tartrate) of the ergot alkaloids, and is stored in this form because the free basic material is too unstable and decomposes readily in the presence of light, heat, moisture and air.
To recover the free base for extraction of the amide of synthesis to LSD, make the tartrate basic with ammonia to pH 9, extract with chloroform and evaporate in vacuo.
If no source of pure Claviceps purpurea fungus can be found, it may be necessary to make a field trip to obtain the ergot growths from rye or other cereal grasses. Rye grass is by far the best choice. The ergot will appear as a blackish growth on the tops of the rye where the seeds are and are referred to as "heads of ergot." From these heads of ergot sprout the Claviceps purpurea fungi. They have long steams with bulbous heads when seen under a strong glass or microscope. It is these that must be removed from the ergot, free from contamination, and used to inoculate the culture media. The need for absolute sterility cannot be overstressed. Consult any elementary text on bacteriology for the correct equipment and procedures. Avoid prolonged contact with ergot compounds, as they are poisonous and can be fatal.
SYNTHESIS : A solution of 6.7 g KOH in 100 mL H2O, under an inert atmosphere and magnetically stirred, was brought to 75 °C, and 10 g ergotamine tartrate (ET) added. The reaction mixture turned yellow as the ergotamine went into solution over the course of 1 h. The stirring was continued for an additional 3 h. The reaction mixture was cooled to about 10 °C with an external ice bath, and acidified to a pH of about 3.0 by the dropwise addition of 2.5 N H2SO4. White solids started to appear early in the neutralization; approximately 60 mL of sulfuric acid was required. The reaction mixture was cooled overnight, the solids removed by filtration, and the filter cake washed with 10 mL Et2O. The dry solids were transferred to a beaker, suspended in 50 mL 15 % ammonia in anhydrous ethanol, stirred for 1 h, and separated by decantation. This extraction was repeated, and the original decantation and the second extract combined and filtered to remove a few hundred milligrams of unwanted solids. The clear filtrate was stripped of solvent under vacuum, the residual solids dissolved in 50 mL of 1% aqueous ammonia, and this solution was acidified as before with 2.5 N H2SO4. The precipitated solids were removed by filtration and washed with Et2O until free of color. After drying under vacuum to a constant weight, there was obtained 3.5 g of d-lysergic acid hydrate, which should be stored in a dark, sealed container.
A suspension of 3.15 g d-lysergic acid hydrate and 7.1 g of diethylamine in 150 mL CHCl3 was brought to reflux with stirring. With the external heating removed, there was added 3.4 g POCl3 over the course of 2 min, at a rate sufficient to maintain refluxing conditions. The mixture was held at reflux for an additional 5 min, at which point everything had gone into solution. After returning to room temperature, the solution was added to 200 mL of 1 N NH4OH. The phases were separated, the organic phase dried over anhydrous MgSO4, filtered, and the solvent removed under vacuum. The residue was chromatographed over alumina with elution employing a 3:1 C6H6/CHCl3 mixture, and the collected fraction stripped of solvent under hard vacuum to a constant weight. This free-base solid can be recrystallized from benzene to give white crystals with a melting point of 87-92 °C. IR (in cm-1): 750, 776, 850, 937 and 996, with the carbonyl at 1631. The mass spectrum of the free base has a strong parent peak at mass 323, with sizable fragments at masses of 181, 196, 207 and 221.
This base was dissolved in warm, dry MeOH, using 4 mL per g of product. There was then added dry d-tartaric acid (0.232 g per g of LSD base), and the clear warm solution treated with Et2O dropwise until the cloudiness did not dispel on continued stirring. This opaqueness set to a fine crystalline suspension (this is achieved more quickly with seeding) and the solution allowed to crystallize overnight in the refrigerator. Ambient light should be severely restricted during these procedures. The product was removed by filtration, washed sparingly with cold methanol, with a cold 1:1 MeOH/Et2O mixture, and then dried to constant weight. The white crystalline product was lysergic acid diethylamide tartrate with two molecules of methanol of crystallization, with a mp of about 200 °C with decomposition, and weighed 3.11 g (66%). Repeated recrystallizations from methanol produced a product that became progressively less soluble, and eventually virtually insoluble, as the purity increased. A totally pure salt, when dry and when shaken in the dark, will emit small flashes of white light.
http://www.shroomery.org/forums/showflat.php/Number/6607718
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them. Doc_T's Efficiency Challenge
|
Agent MadHatter
Mad as a Hatter


Registered: 07/23/09
Posts: 944
Last seen: 10 years, 5 months
|
Re: What would one need to grow Claviceps Paspali? [Re: Numinosum]
#11109346 - 09/22/09 09:39 PM (14 years, 5 months ago) |
|
|
Look guys. I didn't ask how to make LSD, I didn't say I was trying to make LSD, I know what erowid is, and I don't want to know how to grow Purpeura.
I want to know how to cultivate the paspali version.
...god people jump all over you for even mentioning LSD. No wonder no one has it, no one can get any info on it.
But for your information Silly....Making LSD isn't as hard as you think.
-------------------- "May the long-time sun shine upon you And all love surround you And the clear light within you Guide your way home." "Its never too late to start beefing up your obituary"- The Most Interesting Man in the World
|
SillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 2 months
|
Re: What would one need to grow Claviceps Paspali? [Re: Agent MadHatter]
#11109426 - 09/22/09 09:50 PM (14 years, 5 months ago) |
|
|
oh god im such a moron i didn't realize your thread was rhetorical  must have totally thrown you off when i said IF you were trying to make lsd hey but atleast you know so much about LSD that you don't need to know about ergot. cool man have fun! </sarcasm>
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
|
Agent MadHatter
Mad as a Hatter


Registered: 07/23/09
Posts: 944
Last seen: 10 years, 5 months
|
Re: What would one need to grow Claviceps Paspali? [Re: SillyBilly]
#11109450 - 09/22/09 09:52 PM (14 years, 5 months ago) |
|
|
LSD and cultivating ergot are two completely different art forms.
-------------------- "May the long-time sun shine upon you And all love surround you And the clear light within you Guide your way home." "Its never too late to start beefing up your obituary"- The Most Interesting Man in the World
|
|