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Plutonian
Mushroom Man
Registered: 09/20/09
Posts: 1,153
Last seen: 11 years, 4 months
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Powedered latex glove?
#11109283 - 09/22/09 09:31 PM (14 years, 6 months ago) |
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So I'll be using gloves during sterilization, inoculation, and etc steps. But these latex gloves are powdered, are they okay to use? Would the powder contaminate anything?
Thanks.
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Powedered latex glove? [Re: Plutonian]
#11109308 - 09/22/09 09:34 PM (14 years, 6 months ago) |
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I just wash my hand and use hand sanitizer that kills 99% of stuff kinda thing no gloves at all and haven't got a contam. but what ya could do is rub your gloves down with a towel et when there on your hands that have Iso on them
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SillyBilly
Professional
Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 3 months
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Re: Powedered latex glove? [Re: Plutonian]
#11109315 - 09/22/09 09:35 PM (14 years, 6 months ago) |
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nope no need to wear gloves during sterilizing most of the time i don't even use gloves for anything unless i dont want to get my hands dirty you should probably wear gloves though as a precaution since it sounds like you're new to this(?) once you get comfortable with the process of inoculation and you know you wont get contams you can take off the gloves
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
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Plutonian
Mushroom Man
Registered: 09/20/09
Posts: 1,153
Last seen: 11 years, 4 months
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Re: Powedered latex glove? [Re: Shea25]
#11109332 - 09/22/09 09:37 PM (14 years, 6 months ago) |
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Ok thanks, I guess I'll just clean my hands real good and do it without the gloves.
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Ringo
Jupiter
Registered: 08/16/09
Posts: 182
Last seen: 3 years, 5 months
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Re: Powedered latex glove? [Re: Plutonian]
#11109461 - 09/22/09 09:54 PM (14 years, 6 months ago) |
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Powdered gloves are only usually powered on the inside so its easier to put them on correct?
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Powedered latex glove? [Re: Ringo]
#11109486 - 09/22/09 09:57 PM (14 years, 6 months ago) |
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they get powder on the out side may residual powder or on purpose or maybe to help them not stick together
Edited by Shea25 (09/22/09 09:58 PM)
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Powedered latex glove? [Re: Ringo]
#11109504 - 09/22/09 10:00 PM (14 years, 6 months ago) |
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I would never consider sterile work without gloves. Not only that, I wash the gloves themselves with alcohol after putting them on. I always use powdered gloves, since my hands sweat so much. The powder keeps my sweat from dripping out of the back of the gloves and splashing down on the table while I'm working. Powder also makes the gloves easier to get on and off.
Below is a shot of one of my fingernails, taken right after showering, scrubbing my hands and nails with a brush, and washing with alcohol. That bacteria slime which is still left isn't something I want exposed during sterile work. It's important to note that we as cultivators are the number one vector of contamination. Wear a mask to protect your work from the bacteria in your breath too. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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SillyBilly
Professional
Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 3 months
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Re: Powedered latex glove? [Re: Ringo]
#11109507 - 09/22/09 10:01 PM (14 years, 6 months ago) |
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Quote:
Ringo said: Powdered gloves are only usually powered on the inside so its easier to put them on correct?
actually little known fact, rubber companies in the Philippines put very small amounts of anthrax in the powder in a conspiracy with the pharmaceutical lobbyists to keep the public's health low so they can sell more drugs after people get sick thats why people get sick at doctors, not because sick people are already there, but because the doctors touch you with those gloves
yeh but in all honesty you'll be fine once you have gone through the motions enough time you learn when/where to cut corners
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Powedered latex glove? [Re: Shea25]
#11109526 - 09/22/09 10:04 PM (14 years, 6 months ago) |
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I only wear gloves when I make spore syringes or prints and agar work but since making my Self healing lids haven't need gloves for my innocs just wipe the ports with 99% Iso and my hand flame needle and always wear a surgical mask. but agreed its better to go overkill and don the gloves
Edited by Shea25 (09/22/09 10:05 PM)
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Powedered latex glove? [Re: Shea25]
#11109571 - 09/22/09 10:09 PM (14 years, 6 months ago) |
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its a very good idea to wear gloves and clean them with 70% IPA. your shedding dead skin cells all the time which are covered in bacteria.
99% alcohol isnt very effective at killing things. 70% is much better. the water content of the alcohol is important in fooling cells into allowing the alcohol/water mix inside. without enough water, at most the cell dries out becoming dormant.
it may sound a bit overkill, wearing gloves, tyvek sleaves, surgical mask, hairnet. But some of us just hate wasting materials. and its really not much effort.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Powedered latex glove? [Re: Shea25]
#11109589 - 09/22/09 10:11 PM (14 years, 6 months ago) |
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Quote:
just wipe the ports with 99% Iso
70% iso has much more killing power than 99%. That's why we recommend it. Cells admit water through their walls via osmosis, and they can close up the cell wall to prevent entry of toxins. The water mixed with the alcohol 'fools' the cell wall into allowing it to pass, and then the alcohol kills the cell from the inside. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Hmm didnt know that about the 99% compared to the 70% al thoe I have never had a prob with a contam issue. But I do use the hand sanitizers thats kill 99% of stuff I only use the 99% Iso on my hands when I run out. I use the 99% for sterilizing other things besides my hands mostly but maybe now I will have to switch to 70 and its cheaper thanks for the info
Edited by Shea25 (09/22/09 10:14 PM)
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Powedered latex glove? [Re: Shea25]
#11109638 - 09/22/09 10:20 PM (14 years, 6 months ago) |
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use yoiur 99% iso for an alcohol lamp :-)
or you could probably use it in some form of extraction of something
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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badman
Registered: 06/14/06
Posts: 4,039
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A doctor told me a few months back that over exposure the powdered latex gloves can cause allergic reaction even if you aren't allergic to latex. Just a warning
I personally can't stand latex gloves and the powder drives me mad hence I use nitrile gloves.
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Just a quick question wouldn't washing my hands and arms first and then using the 99%work just as well since I dont really give my arms and hands enough time to dry so they still get washed with the H2o and then the 99% Iso ?? Just a question because thats what i do when i dont used my hand sanitizers
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Powedered latex glove? [Re: badman]
#11109781 - 09/22/09 10:43 PM (14 years, 6 months ago) |
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It could but you dont know the what v/v % you will achieve.
dilute your 99% to get 70%
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Powedered latex glove? [Re: badman]
#11109787 - 09/22/09 10:44 PM (14 years, 6 months ago) |
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Hmm I think i would rather buy a new bottle of 70 and save my 99 for a lamp. Why waste a good bottle of 99
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Powedered latex glove? [Re: Shea25]
#11109813 - 09/22/09 10:47 PM (14 years, 6 months ago) |
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Either way i suppose, just a quick fix if you need it.
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Powedered latex glove? [Re: badman]
#11109836 - 09/22/09 10:51 PM (14 years, 6 months ago) |
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True but i have never had a contam with 99% and I have done alot of things, teks made spore syringes, LS's ect.. but it is good knowing this info
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SillyBilly
Professional
Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 3 months
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Re: Powedered latex glove? [Re: Shea25]
#11110321 - 09/23/09 12:19 AM (14 years, 6 months ago) |
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AnTHRAX GLOVES
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
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FoxFire
Energized Matter
Registered: 07/17/05
Posts: 1,281
Loc: PNW
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Re: Powedered latex glove? [Re: SillyBilly]
#11110359 - 09/23/09 12:27 AM (14 years, 6 months ago) |
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Anthrax?!?!?!
I guess... Maybe...
I am 99% sure the powder on the inside of gloves is corn starch. I use them everyday in the kitchen.
If its anthrax well then Im fucked..
-------------------- Unless someone like you cares a whole awful lot, Nothing is going to get better. It's not.
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Quote:
RogerRabbit said: I would never consider sterile work without gloves. Not only that, I wash the gloves themselves with alcohol after putting them on.
-------------------- Gypsum/Drywall Tek The Story Of Russell The Texas (Cube) Bear The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves. -Arnold J. Toynbee
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Powedered latex glove? [Re: SillyBilly]
#11110793 - 09/23/09 03:14 AM (14 years, 6 months ago) |
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Quote:
SillyBilly said: rubber companies in the Philippines put very small amounts of anthrax in the powder
Wait, what?
-------------------- Gypsum/Drywall Tek The Story Of Russell The Texas (Cube) Bear The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves. -Arnold J. Toynbee
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SillyBilly
Professional
Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 3 months
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Re: Powedered latex glove? [Re: nexus1946]
#11110975 - 09/23/09 05:16 AM (14 years, 6 months ago) |
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Quote:
nexus1946 said:
Quote:
SillyBilly said: rubber companies in the Philippines put very small amounts of anthrax in the powder
Wait, what?
yeh dude big brother doesnt want you to know its a huge conspiracy
-------------------- By the livin' Gawd that made you, You're a better man than I am, Gunga Din
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cloudsaregathering
pasturbater
Registered: 08/08/09
Posts: 1,283
Loc: ZZ9 Plural Z Alpha
Last seen: 1 month, 7 days
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Quote:
RogerRabbit said: I would never consider sterile work without gloves. Not only that, I wash the gloves themselves with alcohol after putting them on. I always use powdered gloves, since my hands sweat so much. The powder keeps my sweat from dripping out of the back of the gloves and splashing down on the table while I'm working. Powder also makes the gloves easier to get on and off.
Below is a shot of one of my fingernails, taken right after showering, scrubbing my hands and nails with a brush, and washing with alcohol. That bacteria slime which is still left isn't something I want exposed during sterile work. It's important to note that we as cultivators are the number one vector of contamination. Wear a mask to protect your work from the bacteria in your breath too. RR
Ewwwww...
-------------------- "the root of the problem has been isolated"
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