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OfflinePlutonian
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Registered: 09/20/09
Posts: 1,153
Last seen: 11 years, 4 months
Powedered latex glove?
    #11109283 - 09/22/09 09:31 PM (14 years, 6 months ago)

So I'll be using gloves during sterilization, inoculation, and etc steps. But these latex gloves are powdered, are they okay to use? Would the powder contaminate anything?

Thanks.

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InvisibleShea25
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Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
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Re: Powedered latex glove? [Re: Plutonian]
    #11109308 - 09/22/09 09:34 PM (14 years, 6 months ago)

I just wash my hand and use hand sanitizer that kills 99% of stuff kinda thing no gloves at all and haven't got a contam. but what ya could do is rub your gloves down with a towel et when there on your hands that have Iso on them

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OfflineSillyBilly
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Registered: 03/05/09
Posts: 3,634
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Re: Powedered latex glove? [Re: Plutonian]
    #11109315 - 09/22/09 09:35 PM (14 years, 6 months ago)

nope
no need to wear gloves during sterilizing
most of the time i don't even use gloves for anything
unless i dont want to get my hands dirty
you should probably wear gloves though as a precaution since it sounds like you're new to this(?)
once you get comfortable with the process of inoculation and you know you wont get contams you can take off the gloves


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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OfflinePlutonian
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Registered: 09/20/09
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Re: Powedered latex glove? [Re: Shea25]
    #11109332 - 09/22/09 09:37 PM (14 years, 6 months ago)

Ok thanks, I guess I'll just clean my hands real good and do it without the gloves.

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OfflineRingo
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Registered: 08/16/09
Posts: 182
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Re: Powedered latex glove? [Re: Plutonian]
    #11109461 - 09/22/09 09:54 PM (14 years, 6 months ago)

Powdered gloves are only usually powered on the inside so its easier to put them on correct?


--------------------
:1upmario:

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InvisibleShea25
Just some guy
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Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
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Re: Powedered latex glove? [Re: Ringo]
    #11109486 - 09/22/09 09:57 PM (14 years, 6 months ago)

they get powder on the out side may residual powder or on purpose or maybe to help them not stick together

Edited by Shea25 (09/22/09 09:58 PM)

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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
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Last seen: 1 year, 1 month
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OG Cultivator
Re: Powedered latex glove? [Re: Ringo]
    #11109504 - 09/22/09 10:00 PM (14 years, 6 months ago)

I would never consider sterile work without gloves.  Not only that, I wash the gloves themselves with alcohol after putting them on.  I always use powdered gloves, since my hands sweat so much.  The powder keeps my sweat from dripping out of the back of the gloves and splashing down on the table while I'm working.  Powder also makes the gloves easier to get on and off.

Below is a shot of one of my fingernails, taken right after showering, scrubbing my hands and nails with a brush, and washing with alcohol.  That bacteria slime which is still left isn't something I want exposed during sterile work.  It's important to note that we as cultivators are the number one vector of contamination.  Wear a mask to protect your work from the bacteria in your breath too.
RR



--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineSillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 8 years, 3 months
Re: Powedered latex glove? [Re: Ringo]
    #11109507 - 09/22/09 10:01 PM (14 years, 6 months ago)

Quote:

Ringo said:
Powdered gloves are only usually powered on the inside so its easier to put them on correct?



actually little known fact, rubber companies in the Philippines put very small amounts of anthrax in the powder in a conspiracy with the pharmaceutical lobbyists to keep the public's health low so they can sell more drugs after people get sick
thats why people get sick at doctors, not because sick people are already there, but because the doctors touch you with those gloves:laugh:

yeh but in all honesty you'll be fine
once you have gone through the motions enough time you learn when/where to cut corners


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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InvisibleShea25
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Registered: 01/27/09
Posts: 7,772
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Re: Powedered latex glove? [Re: Shea25]
    #11109526 - 09/22/09 10:04 PM (14 years, 6 months ago)

I only wear gloves when I make spore syringes or prints and agar work but since making my Self healing lids haven't need gloves for my innocs just wipe the ports with 99% Iso and my hand flame needle and always wear a surgical mask. but agreed its better to go overkill and don the gloves

Edited by Shea25 (09/22/09 10:05 PM)

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Offlineveda_sticks
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Re: Powedered latex glove? [Re: Shea25]
    #11109571 - 09/22/09 10:09 PM (14 years, 6 months ago)

its a very good idea to wear gloves and clean them with 70% IPA. your shedding dead skin cells all the time which are covered in bacteria.

99% alcohol isnt very effective at killing things. 70% is much better. the water content of the alcohol is important in fooling cells into allowing the alcohol/water mix inside. without enough water, at most the cell dries out becoming dormant.

it may sound a bit overkill, wearing gloves, tyvek sleaves, surgical mask, hairnet. But some of us just hate wasting materials. and its really not much effort.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
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Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineRogerRabbitM
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Re: Powedered latex glove? [Re: Shea25]
    #11109589 - 09/22/09 10:11 PM (14 years, 6 months ago)

Quote:

just wipe the ports with 99% Iso




70% iso has much more killing power than 99%.  That's why we recommend it.  Cells admit water through their walls via osmosis, and they can close up the cell wall to prevent entry of toxins.  The water mixed with the alcohol 'fools' the cell wall into allowing it to pass, and then the alcohol kills the cell from the inside.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
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Re: Powedered latex glove? [Re: veda_sticks]
    #11109602 - 09/22/09 10:13 PM (14 years, 6 months ago)

Hmm didnt know that about the 99% compared to the 70% al thoe I have never had a prob with a contam issue. But I do use the hand sanitizers thats kill 99% of stuff I only use the 99% Iso on my hands when I run out. I use the 99% for sterilizing other things besides my hands mostly but maybe now I will have to switch to 70 and its cheaper thanks for the info

Edited by Shea25 (09/22/09 10:14 PM)

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Offlineveda_sticks
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Re: Powedered latex glove? [Re: Shea25]
    #11109638 - 09/22/09 10:20 PM (14 years, 6 months ago)

use yoiur 99% iso for an alcohol lamp :-)

or you could probably use it in some form of extraction of something


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Powedered latex glove? [Re: RogerRabbit]
    #11109674 - 09/22/09 10:26 PM (14 years, 6 months ago)

A doctor told me a few months back that over exposure the powdered latex gloves can cause allergic reaction even if you aren't allergic to latex. Just a warning 

I personally can't stand latex gloves and the powder drives me mad hence I use nitrile gloves.

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InvisibleShea25
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Posts: 7,772
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Re: Powedered latex glove? [Re: RogerRabbit]
    #11109703 - 09/22/09 10:31 PM (14 years, 6 months ago)

Just a quick question wouldn't washing my hands and arms first and then using the 99%work just as well since I dont really give my arms and hands enough time to dry so they still get washed with the H2o and then the 99% Iso ?? Just a question because thats what i do when i dont used my hand sanitizers

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Invisiblebadman
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Registered: 06/14/06
Posts: 4,039
Re: Powedered latex glove? [Re: badman]
    #11109781 - 09/22/09 10:43 PM (14 years, 6 months ago)

It could but you dont know the what v/v % you will achieve.

dilute your 99% to get 70%

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InvisibleShea25
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Posts: 7,772
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Re: Powedered latex glove? [Re: badman]
    #11109787 - 09/22/09 10:44 PM (14 years, 6 months ago)

Hmm I think i would rather buy a new bottle of 70 and save my 99 for a lamp. Why waste a good bottle of 99

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Invisiblebadman
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Registered: 06/14/06
Posts: 4,039
Re: Powedered latex glove? [Re: Shea25]
    #11109813 - 09/22/09 10:47 PM (14 years, 6 months ago)

Either way i suppose, just a quick fix if you need it.

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InvisibleShea25
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Re: Powedered latex glove? [Re: badman]
    #11109836 - 09/22/09 10:51 PM (14 years, 6 months ago)

True but i have never had a contam with 99% and I have done alot of things, teks made spore syringes, LS's ect..  but it is good knowing this info

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OfflineSillyBilly
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Registered: 03/05/09
Posts: 3,634
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Re: Powedered latex glove? [Re: Shea25]
    #11110321 - 09/23/09 12:19 AM (14 years, 6 months ago)

AnTHRAX GLOVES


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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InvisibleFoxFire
Energized Matter
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Registered: 07/17/05
Posts: 1,281
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Re: Powedered latex glove? [Re: SillyBilly]
    #11110359 - 09/23/09 12:27 AM (14 years, 6 months ago)

Anthrax?!?!?! 
:ilold:

I guess... Maybe...

I am 99% sure the powder on the inside of gloves is corn starch.  I use them everyday in the kitchen. 

If its anthrax well then Im fucked.. :shrug:


--------------------
Unless someone like you cares a whole awful lot,
Nothing is going to get better.  It's not.

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Offlinenexus1946
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Re: Powedered latex glove? [Re: RogerRabbit]
    #11110784 - 09/23/09 03:10 AM (14 years, 6 months ago)

Quote:

RogerRabbit said:
I would never consider sterile work without gloves.  Not only that, I wash the gloves themselves with alcohol after putting them on. 




:thumbup:


--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee

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Offlinenexus1946
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Re: Powedered latex glove? [Re: SillyBilly]
    #11110793 - 09/23/09 03:14 AM (14 years, 6 months ago)

Quote:

SillyBilly said:
rubber companies in the Philippines put very small amounts of anthrax in the powder




Wait, what? :confused:


--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee

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OfflineSillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
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Re: Powedered latex glove? [Re: nexus1946]
    #11110975 - 09/23/09 05:16 AM (14 years, 6 months ago)

Quote:

nexus1946 said:
Quote:

SillyBilly said:
rubber companies in the Philippines put very small amounts of anthrax in the powder




Wait, what? :confused:



yeh dude big brother doesnt want you to know
its a huge conspiracy


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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Offlinecloudsaregathering
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Re: Powedered latex glove? [Re: RogerRabbit]
    #11112945 - 09/23/09 02:21 PM (14 years, 6 months ago)

Quote:

RogerRabbit said:
I would never consider sterile work without gloves.  Not only that, I wash the gloves themselves with alcohol after putting them on.  I always use powdered gloves, since my hands sweat so much.  The powder keeps my sweat from dripping out of the back of the gloves and splashing down on the table while I'm working.  Powder also makes the gloves easier to get on and off.

Below is a shot of one of my fingernails, taken right after showering, scrubbing my hands and nails with a brush, and washing with alcohol.  That bacteria slime which is still left isn't something I want exposed during sterile work.  It's important to note that we as cultivators are the number one vector of contamination.  Wear a mask to protect your work from the bacteria in your breath too.
RR





Ewwwww...


--------------------

"the root of the problem has been isolated"

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