Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11037836 - 09/10/09 11:48 PM (14 years, 6 months ago)

ok dont know what the hell i did there but that last post was quoteing doc, i was describing farina and its texture


--------------------

Extras: Filter Print Post Top
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11040081 - 09/11/09 11:57 AM (14 years, 6 months ago)

So given the ingredients that i listed describing farina, would possibly if i heated up a couple grains in some water in a shot glass in the micro( of course to rid of any nastys ) and mixed with the usuals, would it possibly be beneficial to the growth of the mycelium if i was doing a culture. I mean since it does like a diverse/variety of stuff to munch on would it do harm,  only asking cause u never know it might love the shit and thrive. Any opinions or thoughts on that?


--------------------

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11040138 - 09/11/09 12:07 PM (14 years, 6 months ago)

I'm sure it would work.
As you try different things, keep the total amount the same. If you add farina, use about as much as you would malt+dextrose or whatever.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11096962 - 09/20/09 08:27 PM (14 years, 6 months ago)

ok so im gonna go and whip up some good ole agar for culture. Im gonna use 9gs of agar ground up mixed with 2tbls of potato flakes but im unsure on how much dextrose(corn sugar) to mix in with it. I got 20 petri dishes so i wanna know how much to use to fill these up, been reading for a couple hours and I cant find a good recipe/mixture to do so.
 
  Any one got a good mixture for what I got to work with? Got no honey and rather not buy any, I wanna use what I got cuz i got alot of it.


--------------------

Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11097061 - 09/20/09 08:46 PM (14 years, 6 months ago)

Heat up your water, add the agar, then give it as much dextrose as what will dissolve easily. I think you'll find it'll only want to take so much before it gets harder to stir it into solution.

i'm gonna guess it'll take about 1-2 tablespoons per cup of hot water+half stick of agar agar before it starts clumping up in there and not wanting to dissolve.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

Edited by anonjon (09/20/09 08:48 PM)

Extras: Filter Print Post Top
InvisibleGetafixx
NotaRoman
Male User Gallery


Registered: 05/10/09
Posts: 524
Loc: 2nd Continent on the Left
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11097099 - 09/20/09 08:51 PM (14 years, 6 months ago)

500ml agar will be enough to pour 20 dishes.
I have had great success with the methods below

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water
Pc for 35-45 minutes

or

Potato Dextrose Agar
use the broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes strain(bring liquid to 500ml again)ie pour the cooked spuds thru a tea strainer and use the remaining liquid for the agar. once cooked bring the liquid back to 500ml by adding water.
then add
9 g agar agar
7 grams dextrose
and some yeast-extract (optional)
to the spud broth and PC for 35-45minutes.


--------------------
Procrastination is like masturbation, when you look at the job in hand you are actually screwing yourself.

Edited by Getafixx (09/20/09 08:59 PM)

Extras: Filter Print Post Top
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: Getafixx]
    #11098065 - 09/20/09 11:59 PM (14 years, 6 months ago)

ya but im using potato flakes instead so that ill try another time


--------------------

Extras: Filter Print Post Top
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: anonjon]
    #11098072 - 09/21/09 12:01 AM (14 years, 6 months ago)

Quote:

anonjon said:
Heat up your water, add the agar, then give it as much dextrose as what will dissolve easily. I think you'll find it'll only want to take so much before it gets harder to stir it into solution.

i'm gonna guess it'll take about 1-2 tablespoons per cup of hot water+half stick of agar agar before it starts clumping up in there and not wanting to dissolve.



  So what about potato flakes? should i use those too, if so how much should i use?


--------------------

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11098882 - 09/21/09 06:06 AM (14 years, 6 months ago)

1/2 teaspoon.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
OfflineNewpala21
1st time farmer
 User Gallery


Registered: 10/16/08
Posts: 371
Last seen: 14 years, 6 months
Re: agar+dextrose-more ingredients needed? [Re: Doc_T]
    #11099679 - 09/21/09 11:03 AM (14 years, 6 months ago)

thanks doc-t


--------------------

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: agar+dextrose-more ingredients needed? [Re: Newpala21]
    #11102552 - 09/21/09 07:51 PM (14 years, 6 months ago)

Did that work out? Is it good? Did you eat a bite?


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Casing Ingredients! Need ASAP help! CraCkmAsTeRstAn 1,386 7 07/03/02 03:26 AM
by BoB
* using agar? downforpot 2,221 6 12/02/01 07:00 PM
by Anno
* What Agar? Maddoc 1,343 7 07/21/01 11:39 AM
by Anonymous
* agar with malted barley syrup Hongosmeester 7,110 17 10/27/19 10:42 PM
by ABCMORPHS
* syringes vs agar Static 1,700 10 06/18/03 06:54 AM
by koraks
* Using Agar medium directly from spore print?? djveiox 7,210 17 12/07/23 03:30 PM
by Screwup
* agar storage. ellisdee625 1,109 4 11/05/02 05:04 AM
by Pinhead
* Spore Syringe on Agar plates Heruuka 2,971 2 09/16/02 06:31 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,406 topic views. 21 members, 114 guests and 158 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.008 seconds on 15 queries.