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OfflineSillyBilly
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    #11096650 - 09/20/09 07:22 PM (14 years, 5 months ago)

.


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By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


Edited by SillyBilly (01/24/10 06:57 AM)


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Invisiblefeelfunny
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Re: pan cyan lc-constant stirring question for blue helix [Re: SillyBilly]
    #11096666 - 09/20/09 07:25 PM (14 years, 5 months ago)

yes that is true they will compete and you will get the better genetics out of the bunch i read RR talking about that a few days ago


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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OfflineSillyBilly
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Re: pan cyan lc-constant stirring question for blue helix [Re: feelfunny]
    #11096715 - 09/20/09 07:35 PM (14 years, 5 months ago)

so maybe, just use agar to clone?

but anyways i feel like there still could be a huge difference between spores in constant motion and lc in constant motion
anyone have any reliable data confirming a favorable one?


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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Invisiblefeelfunny
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Re: pan cyan lc-constant stirring question for blue helix [Re: SillyBilly]
    #11096727 - 09/20/09 07:37 PM (14 years, 5 months ago)

i use to isolate and clone. i was saying that i found a thread saying to put a good bit of spores on the agar so they compete and that is a good way to get a strong isolate instead of a little bit of spores and weak ones can survive.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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Invisiblebadman
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Re: pan cyan lc-constant stirring question for blue helix [Re: feelfunny]
    #11096749 - 09/20/09 07:43 PM (14 years, 5 months ago)

Yeah that is true with the competition and all.

Goliath looks sexy don't it :crazy2:

Correct use of LC is to inoc with a wegde of agar from a monoculture plate. Shooting spores in LCs is a bit blag but we all do it. As for RPM i don't know but from that link it looks like you need to obtain a vortex. If you shoot MS then i would wait until you see myc before stirring via magnet.

Dextrose is active glucose and used in respiration, you could use raw sugar instead.


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OfflineSillyBilly
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Re: pan cyan lc-constant stirring question for blue helix [Re: badman]
    #11096777 - 09/20/09 07:51 PM (14 years, 5 months ago)

so what 2% light malt then 2% sugar(sugar in the raw? or is just regular sugar?)
should i just take a wedge and not isolate it i guess?
just from the first plate then to lc?
thanks guys


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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Offlinedeladude
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Re: pan cyan lc-constant stirring question for blue helix [Re: SillyBilly]
    #11096813 - 09/20/09 07:59 PM (14 years, 5 months ago)

sugar in the raw


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losers always talk about doing their best, winners go home and fuck the prom queen.


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Invisiblebadman
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Re: pan cyan lc-constant stirring question for blue helix [Re: badman]
    #11096827 - 09/20/09 08:01 PM (14 years, 5 months ago)

Not to sure about LME mate but use raw sugar, check this out its sick,

FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed!
http://www.shroomery.org/forums/showflat.php/Number/3077006


To be honest it's entirely up to you how you grow.
My method goes like this; MS to agar, transfer to a new plate, grow, take another wedge/s to make new plates, grow, use wegdes to inoc grain, fruit and the clone the badboys. Obtain a monoculture/s from the clones test the monos, done.


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OfflineSillyBilly
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Re: pan cyan lc-constant stirring question for blue helix [Re: badman]
    #11098776 - 09/21/09 05:10 AM (14 years, 5 months ago)

ok cool guys


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din


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