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Offlineuser_exists
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Registered: 10/05/02
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syringe making fuck up, or not
    #1109584 - 12/04/02 12:03 PM (14 years, 2 days ago)

The first time I made a syringe was about 2 weeks ago the print was small and the spores dropped easily and I used the whole print in my syringe (small print).

This time around I got I bick eq print and the scraping was hard causing prolonged syringe making time also I accidentally sucked up air that I quikly shot out and then finished making the syringe (could this be a problem), I think I might have got tiny (trace) amounts of 70% rubbing alcohol in the syringe will this kill the spores?

please help me out and also post suggestions to getting the spores to come off the print more readily.


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Offlinemoorhsum
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Re: syringe making fuck up, or not [Re: user_exists]
    #1109586 - 12/04/02 12:04 PM (14 years, 2 days ago)

I could be wrong, but when making syringes, some people leave an air bubble in it, to stir the spores around, when the syringe has been laying on it's side for awhile....


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OfflineAzmodeus
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Re: syringe making fuck up, or not [Re: moorhsum]
    #1109722 - 12/04/02 01:21 PM (14 years, 2 days ago)

I did like the EXACT same thing last night.  The spores stuck to the tinfoil..........yes i used tinfoil  :frown:,  but i got enough to make a good syringe after a bit of scraping....i think it'll still be good!


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"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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Offlineuser_exists
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Re: syringe making fuck up, or not [Re: Azmodeus]
    #1109802 - 12/04/02 02:01 PM (14 years, 2 days ago)

thanks for keeping this post up top but I am still looking for an awnser so please does somebody know if the rubbing alcohol will kill my spores and/or will unsterile air cause a problem I don't want to innoculate a bunch of jars with duds.


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OfflineExtravagantDream
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Re: syringe making fuck up, or not [Re: user_exists]
    #1109817 - 12/04/02 02:12 PM (14 years, 2 days ago)

obviously you cant store spores in a pure alc. solution, but I dont think "trace amounts" will do much harm. about the air, I dont think that will matter, I always leave an air bubble. If you dont want to waste the time to make a bunch of jars, Just make one extra one if you are planning on innoculating jars from a diff. syringe. Next time make more than one. I always make like 4+ at a time. Then test each one with one jar.


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: user_exists]
    #1109846 - 12/04/02 02:25 PM (14 years, 2 days ago)



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OfflineExtravagantDream
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Re: syringe making fuck up, or not [Re: vatoloco]
    #1109852 - 12/04/02 02:27 PM (14 years, 2 days ago)

do you sterilize your paper at all?


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: ExtravagantDream]
    #1110083 - 12/04/02 03:48 PM (14 years, 2 days ago)



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OfflineExtravagantDream
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Re: syringe making fuck up, or not [Re: vatoloco]
    #1110095 - 12/04/02 03:54 PM (14 years, 2 days ago)

hehe Thanks for the elaborate story. Just wanted to know if you sterilized the paper or if it was good to use out of the box.


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: ExtravagantDream]
    #1110209 - 12/04/02 04:34 PM (14 years, 2 days ago)



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OfflineExtravagantDream
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Re: syringe making fuck up, or not [Re: vatoloco]
    #1110652 - 12/04/02 06:35 PM (14 years, 2 days ago)

Well, I really don't believe in that beginning, but that's a whole other story. the way I look at it is it's between what someone thought/interpreted/was told and what we have concluded from evidence. I'm a physics major so you decide.


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: ExtravagantDream]
    #1110698 - 12/04/02 06:52 PM (14 years, 2 days ago)



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OfflineBaby_Hitler
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Re: syringe making fuck up, or not [Re: ExtravagantDream]
    #1110725 - 12/04/02 06:59 PM (14 years, 2 days ago)

I'm thinking of majoring in Physics, but I'm not sure what you can do with a bachelors in physics.

Electro-mechanical engineering technology is an easier degree, and it usually pays better too.


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InvisibleBi0TeK
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Re: syringe making fuck up, or not [Re: user_exists]
    #1110882 - 12/04/02 07:50 PM (14 years, 1 day ago)

Theres a very small chance that contams would have been sucked into the syringe so I wouldn't be worried about that at the moment.

Dunno about the small amount of alchohol possibly pulled into the syringe but a month ago or so I went to make another syringe (I've made quite a few!) but due to being intoxicated on weed/booze, managed to bend the needle nearly in half AND pull the plunger out completely !!!

I just drained the syringe into a glass whilst useing the oven tek (although I've heard controversy about oven teks recently) and pulled the solution into a fresh syringe which worked great ! I'm just drying a batch from that lot now !  :grin: 


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineExtravagantDream
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Re: syringe making fuck up, or not [Re: vatoloco]
    #1111615 - 12/04/02 11:02 PM (14 years, 1 day ago)

Naw.. I knew you were just trying to give more information than was needed or desired... therefor trying to be "funny." :tongue: I was just saying I don't share your view of the beginning, that's all. 


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Anonymous

Post deleted by Administrator [Re: user_exists]
    #1111699 - 12/04/02 11:33 PM (14 years, 1 day ago)



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Offlinejoeshitragpicker
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Re: syringe making fuck up, or not [Re: ]
    #1111829 - 12/05/02 12:15 AM (14 years, 1 day ago)

trying to be "funny"
heh


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