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OfflineEN1GMA
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Questions about Liquid Culture
    #11088403 - 09/19/09 01:32 PM (11 years, 7 months ago)

1. Is it better to use 1/2 pint jars or Quart jars for LC?
2. How many cups of water and light corn syrup would you use for those size jars?
3. How much spore solution should you put in the jars?

I have five spore syringes(Burma, Ecuador, Treasure Coast, B+, PES Amazonian) to make some LC with, and I don't want to mess this up. Thanks in advance


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OfflineBrandNewbie
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Re: Questions about Liquid Culture [Re: EN1GMA]
    #11088446 - 09/19/09 01:38 PM (11 years, 7 months ago)



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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Edited by BrandNewbie (09/19/09 01:41 PM)


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Offlinecashus
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Re: Questions about Liquid Culture [Re: BrandNewbie]
    #11088457 - 09/19/09 01:39 PM (11 years, 7 months ago)

"Karo Water" (Corn Syrup Broth)
----------------------------------
1 Teaspoon Light Corn Syrup (Karo Syrup or store brand Light Corn Syrup)
100 ml Purified Water

Mix well until dissolved, sterilize for 20-30 minutes at 15 psi. [17]


found in this

please use the search bar


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Self healing lids
noobies lids


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Offlineoxalic32
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Re: Questions about Liquid Culture [Re: cashus]
    #11089164 - 09/19/09 03:30 PM (11 years, 7 months ago)

I do karo & water or honey & water. I use pint jars. Choose whatever jar you like, you shouldnt need more than a pint of LC.


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Invisiblebotanisthype
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Re: Questions about Liquid Culture [Re: oxalic32]
    #11089337 - 09/19/09 04:00 PM (11 years, 7 months ago)

If your doing a pint - 1-1/2 tblsp. of Karo to 100 ml.

If your doing a quart. 3-5 tablsp. of Karo to 600 ml.

It doesnt matter in size , its just a matter of preference..


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Offlinehazard
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Re: Questions about Liquid Culture [Re: botanisthype]
    #11089368 - 09/19/09 04:07 PM (11 years, 7 months ago)

i have aquestion regarding lc`s i made one the other day and am curious does the myc sink to the bottom or float around? and is it gana be white or off white


Edited by hazard (09/19/09 04:08 PM)


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OfflineEN1GMA
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Re: Questions about Liquid Culture [Re: hazard]
    #11089393 - 09/19/09 04:13 PM (11 years, 7 months ago)

I decided to use 1/2 pint jars, and i used 8-10 grams of light corn syrup to 190 grams of water(sound right?)... then pressure cooked for 15 minutes. They look good, still very clear. Gonna wait till tomorrow to inoculate them. How many cc's would you use, i was thinking bout doing 4cc's per jar because I have 50cc's of spore syringes.


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Offlinehazard
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Re: Questions about Liquid Culture [Re: EN1GMA]
    #11089415 - 09/19/09 04:18 PM (11 years, 7 months ago)

Quote:

EN1GMA said:
I decided to use 1/2 pint jars, and i used 8-10 grams of light corn syrup to 190 grams of water(sound right?)... then pressure cooked for 15 minutes. They look good, still very clear. Gonna wait till tomorrow to inoculate them. How many cc's would you use, i was thinking bout doing 4cc's per jar because I have 50cc's of spore syringes.




from what i read it doesnt take alot of cc`s i used 1cc for a half pint, you only need 2 spores to make it work and theres a ton in a syringe, but im new at this and only have done it twice and you may have more solution i did 100ish cc`s of water and a tsp of honey


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Invisibleexplosiveoxygen
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Re: Questions about Liquid Culture [Re: hazard]
    #11089495 - 09/19/09 04:34 PM (11 years, 7 months ago)

You can always go with honey+ water at about a tesp to a half quart.


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The Great Mycelium (TGMM) is more than you and me, we are all part of One.


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OfflineDiesel_Dawg
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Re: Questions about Liquid Culture [Re: explosiveoxygen]
    #11089599 - 09/19/09 04:59 PM (11 years, 7 months ago)

I would use 2cc max per jar, really you can get away with 1cc, that has worked for me anyway.. I couldn't tell you why but I do know some more informed people have said that the less solution you use the better off your lc would be.


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