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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite, Wild Bird Seed

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OfflineLegoulash
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What am i doing wrong.
    #1108401 - 12/03/02 11:45 PM (14 years, 7 days ago)

    Once again im unsuccesful.My first try i lost 12 pint jars, to nothing..( Nothing grew) This could have been attributed to many things from bunk spores i bought from a store in my city, too dry cakes.  My second attempt was 5  one pint jars that are all gone after a month or so of slowly loosing them to contam.(blue contam). :frown:  .  What are some common nubie problems/fuckups i could be doing?

PS: im doing brf and verm in an incubator at 80f at all times. I spray with lysol before i open the container EVERY TIME and try to be very paranoid about everything when involved in this hobby.
I also boiled the jars for 1 1/2 hours the second time. I now have a pc. 


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Offlinesocratesmind
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Re: What am i doing wrong. [Re: Legoulash]
    #1108518 - 12/04/02 12:35 AM (14 years, 7 days ago)

pc should save alot. flame sterilize needle before innoc. innoc over oven or in open air thats very still with little movement. clean working area with 10% bleach solution and lysol alot. pc should help.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineAngry Mycologist
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Registered: 11/24/02
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Re: What am i doing wrong. [Re: Legoulash]
    #1108527 - 12/04/02 12:37 AM (14 years, 7 days ago)

Give us a list of how you underwent the main procedures.


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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Invisiblefunkymonk
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Re: What am i doing wrong. [Re: socratesmind]
    #1108537 - 12/04/02 12:41 AM (14 years, 7 days ago)

you say pc should work...what is pc?


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OfflineAngry Mycologist
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Re: What am i doing wrong. [Re: funkymonk]
    #1108556 - 12/04/02 12:52 AM (14 years, 7 days ago)

In reply to:

you say pc should work...what is pc?




PC is Pressure Cooker.


--------------------
The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineSkikid16
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Re: What am i doing wrong. [Re: Legoulash]
    #1108654 - 12/04/02 01:24 AM (14 years, 7 days ago)

In reply to:

. What are some common nubie problems/fuckups i could be doing?


I don't know about you specifically, but here's some fuck ups : not waiting long enough to inoculate after sterilizing, not flaming the needle between jars, not sterilizing properly, not haveing a clean environment for inoculation.


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OfflineLegoulash
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Re: What am i doing wrong. [Re: Skikid16]
    #1110587 - 12/04/02 06:11 PM (14 years, 6 days ago)

I don't know about you specifically, but here's some fuck ups : not waiting long enough to inoculate after sterilizing, not flaming the needle between jars, not sterilizing properly, not haveing a clean environment for inoculation.

I waited about 24 hours after sterilizing, mabey longer. i flamed the needle before inoculating with a butane lighter my house isnt that clean (lots of dust and stuff but i inoculated in a small room and washed all the walls and counter tops with 1/10 bleach and water.


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Offlineshirley knott
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Re: What am i doing wrong. [Re: Legoulash]
    #1110615 - 12/04/02 06:21 PM (14 years, 6 days ago)

try half pint jars.
they're supposed to be easier, as they colonise quicker (from 14 days or so for mine) and so cross the finishing post 9eat all the flour0 before contams get a foothold.

Good luck on plan C, dude.


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Offlineviscid
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Re: What am i doing wrong. [Re: Legoulash]
    #1110627 - 12/04/02 06:26 PM (14 years, 6 days ago)

what store sold spores?
what blue contam?

the blue contam sounds the fishiest....are you sure your mycelia wasn't just bruised?

the pc will help, and grains are easier than cakes if you've got a pc.  although starting with cakes is definitely standard around here.

get some spores from a reputable supplier first.....then make sure you didn't just bruise your myc.  i dont know of blue contams.  i'm sure there are some, but i haven't seen any.    some myc just get a little spotty with the blueness.
$.02 :wink: 


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OfflineLegoulash
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Re: What am i doing wrong. [Re: viscid]
    #1110641 - 12/04/02 06:30 PM (14 years, 6 days ago)

naw i dotn think it was bad spores the second time.. Iv heard of many people having sucess with the same species. and i dotn think it was bruising it always started at the top and slowley grew downward.

PS: what did u mean by grain.. Birdseed cakes?


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OfflineBitchCakes
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Re: What am i doing wrong. [Re: Legoulash]
    #1110646 - 12/04/02 06:33 PM (14 years, 6 days ago)

Heres a wild guess...

You didn't use quite enough water, and the little that was there is gone sooner because of the pint jars (instead of 1/2 pint)...the blue contam is probably not a contam, but dry or bruised mycelium.


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Offlineviscid
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Re: What am i doing wrong. [Re: BitchCakes]
    #1126969 - 12/10/02 12:13 AM (14 years, 1 day ago)

zactly.

and birdseed is good.  grains, in general, are good.
:laugh: 


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OfflinelogicalMADNESS
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Registered: 11/10/02
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Re: What am i doing wrong. [Re: Legoulash]
    #1127185 - 12/10/02 01:08 AM (14 years, 1 day ago)

One reason that you might not have seen ANY growth what so ever on your 1/2 pint jars is. The verm layer was too thick and prevented the needle from penetrating the substrate later(that is if you had one) and when you inoc. most of your spore solution was adsorbed by the Verm, I have noticed that on a few of my 1/2 pints due to settling of substrate. i havent seen anyone post this reason for showing no signs of colonization so maybe i could be completely wrong about all of this...


--------------------
"If I were not a physicist, I would probably be a musician. I often think in music. I live my daydreams in music. I see my life in terms of music...I get most joy in life out of music."
-Albert Eistein


Edited by logicalMADNESS (12/10/02 01:09 AM)


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite, Wild Bird Seed

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