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OfflineRelorium
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Registered: 09/04/09
Posts: 13
Last seen: 11 years, 4 months
How much spore solution should I use?
    #11058648 - 09/14/09 06:00 PM (11 years, 7 months ago)

I have 12 one pint jars and only one syringe filled with spore solution. How much solution should I use to inoculate each jar? I'm using the PF tek.


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OfflineProject
Ride the Spiral


Registered: 06/02/09
Posts: 1,429
Last seen: 8 years, 4 months
Re: How much spore solution should I use? [Re: Relorium]
    #11058661 - 09/14/09 06:03 PM (11 years, 7 months ago)

Normally people use 1mL per 1/2 pint, so I'd assume you'd want to use about 2mL per pint.  You can try to use less, but it'll further increase colonization times.  You could do 10 pint jars and use 1mL each; they'd take a while, which would increase contamination risk, but they'd have a good chance of still working out.

That's my opinion.  Wait for others...


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OfflineCaliforniaLove
BlackIce
Male


Registered: 08/08/09
Posts: 244
Loc: San Francisco Bay Area
Last seen: 10 years, 6 months
Re: How much spore solution should I use? [Re: Relorium]
    #11058662 - 09/14/09 06:03 PM (11 years, 7 months ago)

how large is the syringe?


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Offlinechillywilly419
Arctic Plumber


Registered: 05/15/09
Posts: 193
Last seen: 11 years, 6 months
Re: How much spore solution should I use? [Re: Relorium]
    #11058685 - 09/14/09 06:06 PM (11 years, 7 months ago)

try a quarter of a cc per inoculation hole, 1 cc of solution per jar. hopefully, your 10 cc syringe is filled to the 12 cc capacity. when inoculating, you really only need a small drop per hole. Hope that helps!!


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Invisiblecurbstop
Regnarts


Registered: 06/02/09
Posts: 810
Re: How much spore solution should I use? [Re: Relorium]
    #11058689 - 09/14/09 06:06 PM (11 years, 7 months ago)

If you have a PC sterilize a pint of water, When you get about half way done with the syringe fill it back up with the sterilized water.
If you go this route make sure you are in sterile conditions:thumbup:.
If you dont, then simply use just enough to where you see the juice hit the sub then go to the next.
You never need very much to accomplish what we're doing:thumbup:


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is multispore a spore company


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OfflineRelorium
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Registered: 09/04/09
Posts: 13
Last seen: 11 years, 4 months
Re: How much spore solution should I use? [Re: Relorium]
    #11058705 - 09/14/09 06:10 PM (11 years, 7 months ago)

Ok, thanks guys. I'll probably end up posting pics of my first flush. Not that it's anything you haven't seen already. :P


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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