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rmh26
Stranger

Registered: 07/29/09
Posts: 37
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Microscope Uses
#11040470 - 09/11/09 01:02 PM (14 years, 5 months ago) |
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So I found an old microscope 400X and was wondering how if it could help in mycology besides looking at spores. IE can I look at mycelium or take a sample from a lc and determine if I have cube mycelium or something else. Does anyone have a link to pictures of magnified mycelium.
Online links would be preferred to a book suggestion as I'm am low of cash right now.
thanks
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Microscope Uses [Re: rmh26]
#11040483 - 09/11/09 01:04 PM (14 years, 5 months ago) |
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When you get cash, buy a copy of TMC. Lots of good microscopy in there.
-------------------- You make it all possible. Doesn't it feel good?
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rmh26
Stranger

Registered: 07/29/09
Posts: 37
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Re: Microscope Uses [Re: Doc_T]
#11041773 - 09/11/09 05:04 PM (14 years, 5 months ago) |
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realizes he has a copy of TMC on his computer that he never got around to reading.
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RogerRabbit
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Registered: 03/26/03
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Re: Microscope Uses [Re: rmh26]
#11042160 - 09/11/09 06:25 PM (14 years, 5 months ago) |
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Here's mycelium(hyphae-note the branching already), about an hour after germinating from the spore.

Here's a few cells of mycelium with the focus adjusted to zero in on the nuclei. You can see two to three in each cell. This is what we call dikaryotic mycelium, meaning it's capable of forming fruit bodies. If there had only been one nucleus per cell, it would be like the mycelium in the first picture, that hadn't yet 'mated', or monokaryotic mycelium.

You'll find lots of pictures taken through microscopes. I know I've posted at least a hundred over the years. Use the 'search posts' feature with the search term microscope and the username of whomever you want to search. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Mykologist
Often too lazy to post...



Registered: 10/06/08
Posts: 1,586
Loc: I'm still here...
Last seen: 4 days, 16 hours
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Hmmm I have a good, solid microscope. RR, how did you germinate the spore on the slide? Is it just a luck thing? Do you add nutrients the water?
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  Where would you rather be?
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ratdog
wild capture trader



Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Microscope Uses [Re: rmh26]
#11043434 - 09/11/09 10:46 PM (14 years, 5 months ago) |
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hay good find i got one chepo needs work but with web cam i got this
wild oyster spores

hoping to get them to grow
neat stuff and a big help in identifying shrooms

sorry better pic
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
Edited by ratdog (09/11/09 11:00 PM)
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
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Quote:
Mykologist said: Hmmm I have a good, solid microscope. RR, how did you germinate the spore on the slide? Is it just a luck thing? Do you add nutrients the water?
I make slides with 4% karo mixed with 96% water. Put the spores down on one drop of karo/water solution, then lay a cover slip over that. Spores start to germinate within an hour or two. You can see about 30 pictures like the above in my gallery, taken every 20 minutes as the hyphae expands and grows.
Cheapie microscopes are really not worth the money. Invest in a pressure cooker and flowhood instead. When you get ready to move up to microscopy, get a good scope with large objectives. A decent scope will set you back $2K or more, but is worth it. I have two or three $500 scopes sitting out in the barn getting bird shit on them. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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kydelic
Stranger



Registered: 06/08/09
Posts: 371
Last seen: 12 years, 8 months
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This is why I love going to a university. When I get through with organizing and doing the work I need for my mycology class I'll be posting up my microscopy. I'm not sure how interested people will be in any other fungi besides the basidiomycetes, but I suppose we shall see. I love microscopy!
-------------------- http://flickr.com/photos/mycography - A collection of my fungal photography
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ratdog
wild capture trader



Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Microscope Uses [Re: kydelic]
#11047114 - 09/12/09 03:42 PM (14 years, 5 months ago) |
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RR please let me rescue that shitty scope from the birds i know they are not using it.
Quote:
Cheapie microscopes are really not worth the money. Invest in a pressure cooker and flowhood instead. When you get ready to move up to microscopy, get a good scope with large objectives. A decent scope will set you back $2K or more, but is worth it. I have two or three $500 scopes sitting out in the barn getting bird shit on them.
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Quote:
RogerRabbit said: I make slides with 4% karo mixed with 96% water.
Where do you get your 96% water and 4% karo? All I can find is 100%.
-FF
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ratdog
wild capture trader



Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Microscope Uses [Re: fastfred]
#11048288 - 09/12/09 07:50 PM (14 years, 5 months ago) |
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Quote:
fastfred said:
Quote:
RogerRabbit said: I make slides with 4% karo mixed with 96% water.
Where do you get your 96% water and 4% karo? All I can find is 100%.
-FF
LMFAO
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Quote:
RogerRabbit said: A decent scope [...] is worth it. RR
Yeah. I don't own a microscope, but the same thing applies to telescopes. The $500 one is just enough to make you want the $2000 one.
-------------------- You make it all possible. Doesn't it feel good?
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ratdog
wild capture trader



Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Microscope Uses [Re: Doc_T]
#11048360 - 09/12/09 08:02 PM (14 years, 5 months ago) |
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true
having been a lab tech for 10 years i know what i should use "Zeiss" but now were can i get a government loan to get one and shipping is another mater. man i want a better scope i bet a bird shit one is better than the one i got now.
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 5 days
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Re: Microscope Uses [Re: fastfred]
#11048509 - 09/12/09 08:30 PM (14 years, 5 months ago) |
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Quote:
fastfred said:
Quote:
RogerRabbit said: I make slides with 4% karo mixed with 96% water.
Where do you get your 96% water and 4% karo? All I can find is 100%.
-FF
Got me. I think everyone knows what I meant to say though...Brain farted. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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