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Amazon Shop: ½ Pint Jars, Agar

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OfflineErbaliza
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Registered: 08/31/02
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Loc: UK...London(ish)
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Agar....Re-using question
    #1103255 - 12/02/02 07:38 AM (14 years, 6 months ago)

I made up a batch of agar,poured my dishes and had a lot left in the glass container.I fridged it overnight to keep it fresh.The question is can i turn it to liquid again once it has set? Or is it easier to make up another batch...Peace


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OfflineDERRAYLD
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Registered: 05/13/02
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Re: Agar....Re-using question [Re: Erbaliza]
    #1103260 - 12/02/02 07:48 AM (14 years, 6 months ago)

Yes it is totally reusable.
Just pop it in the pc when you do more jars and pour it as usual.
Or you could reheat it in a pot with boiled water like your pf jars.
Only really needs a coule minutes to liquify, as long as you store it in sterile conditions.


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Derrayld and out of control


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: DERRAYLD]
    #1103265 - 12/02/02 07:58 AM (14 years, 6 months ago)



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OfflineDERRAYLD
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Re: Agar....Re-using question [Re: matts]
    #1103266 - 12/02/02 08:01 AM (14 years, 6 months ago)

Pretty much any form of heat will do it.
Just need to liquify the solid mass with heat.


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Derrayld and out of control


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OfflineErbaliza
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Re: Agar....Re-using question [Re: DERRAYLD]
    #1103273 - 12/02/02 08:09 AM (14 years, 6 months ago)

Thanks for the info :smile: Will pop it in the PC later when i make up some more honey water....Peace 


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Offlinerhizo
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Registered: 10/31/02
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Re: Agar....Re-using question [Re: Erbaliza]
    #1103277 - 12/02/02 08:13 AM (14 years, 6 months ago)

what are you doing with your agar? cloning? isolation from multispore?


--------------------
An optimist is never pleasantly surprised.


Edited by rhizo (12/02/02 08:16 AM)


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OfflineDERRAYLD
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Re: Agar....Re-using question [Re: rhizo]
    #1103283 - 12/02/02 08:19 AM (14 years, 6 months ago)

Does it make a difference Rhizo?
Unless ofcourse you are thinking that the peroxide will be broken down if re-heated??
In that case I`d guess just adding more Peroxide at the correct time will be fine.



--------------------
Derrayld and out of control


Edited by DERRAYLD (12/02/02 08:20 AM)


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OfflineErbaliza
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Re: Agar....Re-using question [Re: rhizo]
    #1103286 - 12/02/02 08:21 AM (14 years, 6 months ago)

Sectoring i guess....My 1st time with agar.Got some P.Sem myc growing in honey water and have been innoc'ing some agar dishes with it as the print was taken from the wild and not too sterile...Peace

PS...Havnt used any peroxide,just agar,potato broth and dextrose...


Edited by Erbaliza (12/02/02 08:23 AM)


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Offlinerhizo
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Re: Agar....Re-using question [Re: DERRAYLD]
    #1103290 - 12/02/02 08:26 AM (14 years, 6 months ago)

oh sorry, didn't mean to throw you, i was just curious. i'm just now getting into agar myself and was wondering what he's up to with it

Erbaliza, sounds cool dude. hope you get a clean culture


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An optimist is never pleasantly surprised.


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Amazon Shop: ½ Pint Jars, Agar

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