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Post deleted by Administrator
    #1102706 -


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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1102726 -

some people have used h2o2 solutions in there grains i've heard. chemically it is just different than water by the extra oxygen molecule :/ some people that have used it in growlogs are right here i know.
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Growlog&Number=609532&page=&view=&sb=&o=&fpart=1&vc=1


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.

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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1103129 -

Straight peroxide? That's rediculous. Was it 3% or 6%?
I would dilute it to at least 1:10, more likely 1:20
When I make peroxidated agar I use about 1ml of 6% peroxide in 250ml of agar


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Re: How bad is H2O2 for mycelial tissue [Re: zeta]
    #1103137 -

In reply to:
When I make peroxidated agar I use about 1ml of 6% peroxide in 250ml of agar



Well your ratio sounds like 1:16 and works well i guess so that must be a good range.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.

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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1103200 -

i innoculated a mildly peroxidated (1ml @3% / 500ml of purified water) MYA mix w/ flammulina velutipes i got from anno. about a week later i innoculated MYA w/ no peroxide. the the non peroxidated plates are growing quicker as i would expect from the info provided on these boards, w/ no contams. out of the 6 peroxidated plates i started, 4 show slow but steady growth, the other 2 sit idle, i'm just hanging onto them for now... maybe the growing mycelia have been damaged in some way. i don't know. i hope to get a microscope soon... not sure if it will make it here before christmas. i'll probably preserve samples of each and see if there is a physical difference when i have one... maybe someone else knows the answer already. my work was done w/ spores...

u are using tissue samples so there's a big difference already. but straight up h2o2 is pretty kick ass from what i understand. if the oxidation damaged the outer layer of ur sample it may be all over.. i'll put this thread in my favorite, please keep us posted.

peace!


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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1104112 -

Peroxide can be used in agar, grains, and tissue sampling. For general use in a substrate (agar, grain etc.) you want a total peroxide content of .15% by weight.

In this case you're simply rinsing the tissue. 3% peroxide which is the most common concentration from a drug store in the US is fine for brief rinsings. It will bubble like crazy, have you never poured peroxide on an open wound before? That's what the stuff does, and is why it's used as an antiseptic.

You should be perfectly fine, assuming everything else was done properly.

BTW, I have extensive knowledge of peroxide if anybody has specific questions.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1104575 -

well i learned something today... thank u mycofile...

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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1104986 -

Yeah hydrogen peroxide is pretty harmless to mycelium and fruit bodies. I have even heard of a tek that uses h202 for its sterilization method.


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: How bad is H2O2 for mycelial tissue [Re: SixCee]
    #1105231 -

mmmmm, elements, percentages..... its good to see more chemistry on the boards.


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"Its not not a dollar bill" All statements for entertainment porposes only.

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Re: How bad is H2O2 for mycelial tissue [Re: SixCee]
    #1106408 -

i've read the same thing... but i was under the impression even 3% solution direct expousure could damage mycelia. this is a good thing to know. the FV i'm culturing on peroxide is slow... but steady. another species spores just sit in the dish. no sign of contams, but no colinization either... i'm sitting tight for now. the teks that use peroxide seem pretty cool and the benefits from breakdown makes so much sense. i'm learning alot here...

one thing that i did do that wasn't mentioned... i don't want to derail this thread, but maybe someone can quickly comment on this fact and it's roll in my results so far...

i used 1.5 grams of yeast in a 500 ml batch.  :crazy: did i make bad?

thanks sixcee.  :cool:   

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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1106417 -

Recomended is 1g per 500ml but you can use more without problems.

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Post deleted by Administrator [Re: Anonymous]
    #1106660 -


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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1107023 -

hi mycofile, i love peroxide myself, and i would like to take advantage of your offer to share on the topic. my specific questions are:

1. peroxide & grains. could explain how you do it? do you mix h2o2 with the grains before or after sterilization? please give all details;

2. peroxide & pan cyans. please note if there are any differences with respect to h2o2 & pcubensis.

thank you, i look forward to your precious knowledge :laugh: 


--------------------
comario


"crusaders against emotional poverty"

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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1107296 -

amazonians stalled on peroxide on me... any idea's mycofile? thank u in advance..

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Post deleted by Administrator [Re: ]
    #1115378 -


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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1117172 -

Yeah, i did some jars recently....and by day 5 was getting pretty nervous, but then low and behold!! patience is a virtue!


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"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "

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Re: How bad is H2O2 for mycelial tissue [Re: Azmodeus]
    #1117505 -

h202 doesnt seem to hurt it to bad, ive mixed some with water before and used to to treat the cobweb mold, works most of the time if you catch it early

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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1118312 -

I use a 3% sol'n of peroxide in the same manner you did when I prepare cultures from wild specimens.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1119299 -

How strong peroxide solution would be needed for full desinfection?


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Nothing lasts, nothing is lost.

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Re: How bad is H2O2 for mycelial tissue [Re: scifi5001]
    #1119518 -

the recommended dilution is 20:1,
water : 3% peroxide from the bottle.


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Admin @ mycotopia.net
Mycotopia

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Re: How bad is H2O2 for mycelial tissue [Re: comario2]
    #1134460 -

Peroxide and grains:
h202 mixed in after sterilization of course (otherwise the heat breaks down the peroxide). Shoot for .15% peroxide concentration in the grain. Use 10-20 cc's 3% peroxide per quart jar if you don't want to do the math. Innoculate with some type of mycelium, grain to grain, agar etc. I've been getting a lot of ?s on this, so maybe I'll do a formal write up.

2. Peroxide and pans, yes it works. Pans don't seem to do well with quite as high concentrations as cubensis. Cubes seem to take a very high concentration very well, whereas the pans will stall longer and at lower concentrations.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1134479 -

>Use 10-20 cc's 3% peroxide per quart jar if you don't want to do the math.

Don?t forget though to prepare the jars rather on the dry side if you are going to add that much liquid....


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1134484 -

All strains stall on peroxide, perhaps they just needed a few more days.
More likely you just had the peroxide content a little high. Lower the concentration a little and try again. This is usually the case when a culture stalls (stalls as in quits growing altogethor, not just the lag period while it accustoms itself to the peroxide).


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1134492 -

5 days isn't that long when cloning directly from fruit. Remember that the fungus is geared towards one thing, spore production, when you take it and put it back into a substrate, it has to change gears back to vegetative growth and accustom itself to the peroxide at the same time.

Congratulations on the (near) successful clone.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: scifi5001]
    #1134499 -

IMO, peroxide is not effective at full sterilization. The word disinfection itself denotes that full sterilization is acheived. Disinfection is the elimination of a certain number or kind of organism. IMO so far as treating a substrate to render it clean enough to cultivate on, peroxide is not effective. Heat must be used to sterilize or pasteurize (aka disinfect). Then peroxide is used to keep the substrate contaminate free.

Wayne does describe a method of preparing a substrate with peroxide alone and no heat, but it's not readily applicable to our specific species and paramaters IMO.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: Anno]
    #1136111 -

true indeed. but even at that, it depends how anal you are. Also, ime at what temp you incubate. A little extra water as provided by these peroxide aditions even in a rather moist grain doesn't seem to matter as much at 75 deg F as much as it matters at 84 deg F. People seem to forget that cultivation is about favoritism. 84 deg favors bacteria as much as it does mycelium. Excess moisture favors bacteria more than mycelium. Cultivation is about balancing favoritism towards mycelium, the trade off's are each cultivator's decision.

If you want an overly wet substrate, fine. Just don't try to incubate it at high temps. This type of thinking applies all throughout the cultivation process. But then again, the person who isn't going to account for the added water in 12 cc's of peroxide, probbly isn't anal enough to incubate in the first place. Or was that just me?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Mushroom-Hut Mono Tub Substrate   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Advanced Mycology
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