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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: How bad is H2O2 for mycelial tissue [Re: comario2]
    #1134460 -

Peroxide and grains:
h202 mixed in after sterilization of course (otherwise the heat breaks down the peroxide). Shoot for .15% peroxide concentration in the grain. Use 10-20 cc's 3% peroxide per quart jar if you don't want to do the math. Innoculate with some type of mycelium, grain to grain, agar etc. I've been getting a lot of ?s on this, so maybe I'll do a formal write up.

2. Peroxide and pans, yes it works. Pans don't seem to do well with quite as high concentrations as cubensis. Cubes seem to take a very high concentration very well, whereas the pans will stall longer and at lower concentrations.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1134479 -

>Use 10-20 cc's 3% peroxide per quart jar if you don't want to do the math.

Don?t forget though to prepare the jars rather on the dry side if you are going to add that much liquid....


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1134484 -

All strains stall on peroxide, perhaps they just needed a few more days.
More likely you just had the peroxide content a little high. Lower the concentration a little and try again. This is usually the case when a culture stalls (stalls as in quits growing altogethor, not just the lag period while it accustoms itself to the peroxide).


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1134492 -

5 days isn't that long when cloning directly from fruit. Remember that the fungus is geared towards one thing, spore production, when you take it and put it back into a substrate, it has to change gears back to vegetative growth and accustom itself to the peroxide at the same time.

Congratulations on the (near) successful clone.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: scifi5001]
    #1134499 -

IMO, peroxide is not effective at full sterilization. The word disinfection itself denotes that full sterilization is acheived. Disinfection is the elimination of a certain number or kind of organism. IMO so far as treating a substrate to render it clean enough to cultivate on, peroxide is not effective. Heat must be used to sterilize or pasteurize (aka disinfect). Then peroxide is used to keep the substrate contaminate free.

Wayne does describe a method of preparing a substrate with peroxide alone and no heat, but it's not readily applicable to our specific species and paramaters IMO.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: How bad is H2O2 for mycelial tissue [Re: Anno]
    #1136111 -

true indeed. but even at that, it depends how anal you are. Also, ime at what temp you incubate. A little extra water as provided by these peroxide aditions even in a rather moist grain doesn't seem to matter as much at 75 deg F as much as it matters at 84 deg F. People seem to forget that cultivation is about favoritism. 84 deg favors bacteria as much as it does mycelium. Excess moisture favors bacteria more than mycelium. Cultivation is about balancing favoritism towards mycelium, the trade off's are each cultivator's decision.

If you want an overly wet substrate, fine. Just don't try to incubate it at high temps. This type of thinking applies all throughout the cultivation process. But then again, the person who isn't going to account for the added water in 12 cc's of peroxide, probbly isn't anal enough to incubate in the first place. Or was that just me?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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